Purification and Application of Genetically Encoded Potassium Ion Indicators for Quantification of Potassium Ion Concentrations within Biological Samples [0.03%]
用于生物样本中钾离子浓度定量的基因编码钾离子指示剂的纯化与应用
H Bischof,S Burgstaller,N Vujic et al.
H Bischof et al.
Vital cells maintain a steep potassium ion (K+ ) gradient across the plasma membrane. Intracellular potassium ion concentrations ([K+ ]) and especially the [K+ ] within the extracellular matrix are strictly regulated, the latter within a na...
Cell Culture and Coculture for Oncological Research in Appropriate Microenvironments [0.03%]
适宜微环境下的肿瘤研究的细胞培养与共培养技术进展
Apekshya Chhetri,Shirisha Chittiboyina,Farzaneh Atrian et al.
Apekshya Chhetri et al.
With the increase in knowledge on the importance of the tumor microenvironment, cell culture models of cancers can be adapted to better recapitulate physiologically relevant situations. Three main microenvironmental factors influence tumor ...
Novel Approaches to Access Arylfluorosulfates and Sulfamoyl Fluorides Based on Sulfur (VI) Fluoride Exchange [0.03%]
基于硫酰六氟氯交换的芳基氟磺酸酯和亚磺酰氟的新合成方法学研究
Zilei Liu,Genyi Meng,Taijie Guo et al.
Zilei Liu et al.
Sulfur (VI) fluoride exchange (SuFEx) is a new family of click chemistry reactions that relies on readily available sulfuryl fluoride (SO2 F2 ) and ethenesulfonyl fluoride to build diverse chemical structures bearing the SVI -F motif, such ...
Biochemical Stimulation of Immune Cells and Measurement of Mechanical Responses Using Atomic Force Microscopy [0.03%]
采用原子力显微镜测定免疫细胞对化学刺激的机械反应
Kenneth H Hu,Marc A Bruce,Jianwei Liu et al.
Kenneth H Hu et al.
This manuscript details methods to ligate cell-surface receptors on live cells with precise spatiotemporal control using an atomic force microscope (AFM) to deliver ligands. This approach can be used to image cellular responses upon activat...
Enzymatic Synthesis of Backbone-Modified Oligonucleotides Using T4 DNA Ligase [0.03%]
T4DNA连接酶催化合成主链修饰的寡核苷酸
Donaat Kestemont,Piet Herdewijn,Marleen Renders
Donaat Kestemont
T4 DNA ligase in high concentrations of certain crowding agents and cosolutes catalyzes the synthesis of a series of backbone-modified oligonucleotides that are difficult to obtain chemically. Backbone-modified nucleic acids are often enzym...
Philip A Cistrone,Michael J Bird,Dillon T Flood et al.
Philip A Cistrone et al.
For over 20 years, native chemical ligation (NCL) has played a pivotal role in enabling total synthesis and semisynthesis of increasingly complex peptide and protein targets. Classical NCL proceeds by chemoselective reaction of two unprotec...
In Vivo Identification of Protein Kinase Substrates by Kinase-Oriented Substrate Screening (KIOSS) [0.03%]
体内蛋白质激酶底物的在体识别：导向底物筛选(KIOSS)方法
Tomoki Nishioka,Mutsuki Amano,Yasuhiro Funahashi et al.
Tomoki Nishioka et al.
Protein phosphorylation plays a critical role in the regulation of cellular function. Information on protein phosphorylation and the responsible kinases is important for understanding intracellular signaling. A method for in vivo screening ...
Lars David Renner
Lars David Renner
This article describes the design and fabrication of microchambers that are used for the study of bacterial cells. The design allows for the confinement and precise manipulation of bacterial cell shape. The application of fluorescent dyes a...
Quantification of Cellular Proteostasis in Live Cells by Fluorogenic Assay Using the AgHalo Sensor [0.03%]
用于活细胞中蛋白质稳态定量的荧光测定法AgHalo传感器
Matthew Fares,Xin Zhang
Matthew Fares
Proper cellular proteostasis is essential to cellular fitness and viability. Exogenous stress conditions compromise proteostasis and cause aggregation of cellular proteins. We have developed a fluorogenic sensor (AgHalo) to quantify stress-...
A Sensitive High-Throughput Screening Method for Identifying Small Molecule Stimulators of the Core Particle of the Proteasome [0.03%]
一种用于识别蛋白酶体核心颗粒刺激物的高通量筛选方法
Rachel A Coleman,Darci J Trader
Rachel A Coleman
Fluorescence resonance energy transfer (FRET) technology is a useful tool to monitor protein interactions as well as protease activity. We have recently reported a biochemical assay utilizing a FRET reporter peptide to monitor the activity ...