Ratiometric Single-Molecule FRET Measurements to Probe Conformational Subpopulations of Intrinsically Disordered Proteins [0.03%]
比率单分子FRET测量探究内在无序蛋白构象亚群性质
Irem Nasir,Emily P Bentley,Ashok A Deniz
Irem Nasir
Over the past few decades, numerous examples have demonstrated that intrinsic disorder in proteins lies at the heart of many vital processes, including transcriptional regulation, stress response, cellular signaling, and most recently prote...
Detection of Small-Molecule Aggregation with High-Throughput Microplate Biophysical Methods [0.03%]
采用高通量微孔板生物物理方法检测小分子聚集现象
Samantha J Allen,Corey M Dower,Annie X Liu et al.
Samantha J Allen et al.
Small-molecule drug discovery can be hindered by the formation of aggregates that act as non-selective inhibitors of drug targets. Such aggregates appear as false positives in high-throughput screening campaigns and can bedevil structure-ac...
N-Terminal Modification of Proteins with Subtiligase Specificity Variants [0.03%]
利用变异芽孢杆菌蛋白酶Subtiligase进行蛋白质N端定点修饰
Amy M Weeks,James A Wells
Amy M Weeks
Subtiligase is a powerful enzymatic tool for N-terminal modification of proteins and peptides. In a typical subtiligase-catalyzed N-terminal modification reaction, a peptide ester donor substrate is ligated onto the unblocked N terminus of ...
Synthesis and Application of an Activity-Based Peptide-Peptoid Hybrid Probe for the Immunoproteasome [0.03%]
一种用于免疫蛋白酶体的肽-类肽混合探针的合成与应用
Breanna L Zerfas,Darci J Trader
Breanna L Zerfas
The immunoproteasome (iCP), a specific isoform of the proteasome's catalytic particle, is becoming an important protein complex of interest in various diseases. However, there is still much left to be learned about its activity in cells and...
Metabolic Incorporation of N-Acetyl Muramic Acid Probes into Bacterial Peptidoglycan [0.03%]
N-乙酰胞壁酸探针的代谢掺入细菌肽聚糖中的研究
Kristen E DeMeester,Hai Liang,Junhui Zhou et al.
Kristen E DeMeester et al.
Bacterial cells utilize small carbohydrate building blocks to construct peptidoglycan (PG), a highly conserved mesh-like polymer that serves as a protective coat for the cell. PG production has long been a target for antibiotics, and its br...
Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale [0.03%]
基于光亲和标记的全蛋白组小分子互作图谱绘制技术(SIM-PAL)鉴定结合蛋白质的小分子位点
Hope A Flaxman,David K Miyamoto,Christina M Woo
Hope A Flaxman
Identification and characterization of small molecule-protein interactions is critical to understanding the mechanism of action of bioactive small molecules. Photo-affinity labeling (PAL) enables the capture of noncovalent interactions for ...
Proteomic Detection and Validation of Translated Small Open Reading Frames [0.03%]
小型开放阅读框转录本的蛋白质组学检测与验证
Alexandra Khitun,Sarah A Slavoff
Alexandra Khitun
Small open reading frames (smORFs) encode previously unannotated polypeptides or short proteins that regulate translation in cis (eukaryotes) and/or are independently functional (prokaryotes and eukaryotes). Ongoing efforts for complete ann...
Activity-Based Kinome Profiling Using Chemical Proteomics and ATP Acyl Phosphates [0.03%]
基于化学蛋白质组学和ATP酰基磷酰化合物的活性依赖性激酶谱分析
Caroline E Franks,Ku-Lung Hsu
Caroline E Franks
Human kinases are a large family of proteins (500+) that catalyze ATP-dependent phosphorylation of protein and metabolite substrates to regulate diverse facets of cell biology. Dysregulation and mutations of protein kinases are linked to hu...
Elucidating Compound Mechanism of Action and Predicting Cytotoxicity Using Machine Learning Approaches, Taking Prediction Confidence into Account [0.03%]
考虑预测信心的机器学习方法阐明复合物的作用机制和预测细胞毒性
Georgios Drakakis,Isidro Cortés-Ciriano,Ben Alexander-Dann et al.
Georgios Drakakis et al.
The modes of action (MoAs) of drugs frequently are unknown, because many are small molecules initially identified from phenotypic screens, giving rise to the need to elucidate their MoAs. In addition, the high attrition rate for candidate d...
Louis J Papa rd,Matthew D Shoulders
Louis J Papa rd
Recombineering inserts PCR products into DNA using homologous recombination. A pair of short homology arms (50 base pairs) on the ends of a PCR cassette target the cassette to its intended location. These homology arms can be easily introdu...