Chutima Petchprayoon,Gerard Marriott
Chutima Petchprayoon
Optical switches represent a new class of molecular probe with applications in high contrast imaging and optical manipulation of protein interactions. Small molecule, organic optical switches based on nitrospirobenzopyran (NitroBIPS) and th...
Derivatization of free natural glycans for incorporation onto glycan arrays: derivatizing glycans on the microscale for microarray and other applications (ms# CP-10-0194) [0.03%]
自由糖缀合物的衍生化以结合到糖芯片上：微升规模下寡糖的衍生化及其在芯片和其它应用中的利用（摘要号：CP-10-0194）
Xuezheng Song,Jamie Heimburg-Molinaro,David F Smith et al.
Xuezheng Song et al.
Nature possesses an unlimited number and source of biologically-relevant natural glycans, many of which are too complicated to synthesize in the laboratory. To capitalize on the naturally-occurring plethora of glycans, we have developed a m...
High-throughput assessment of bacterial growth inhibition by optical density measurements [0.03%]
基于光密度测量的高效抗生素抑菌筛选方法研究细菌生长抑制作用
Jennifer Campbell
Jennifer Campbell
The increasing incidence of antibiotic-resistant bacterial infections both in hospitals and in the community intensifies the need for new antibacterial strategies and targets. Although high-throughput screening against live bacteria allows ...
Robert D Kuchta
Robert D Kuchta
Nucleotide analogues represent a major class of anti-cancer and anti-viral drugs, and provide an extremely powerful tool for dissecting the mechanisms of DNA and RNA polymerases. While the basic assays themselves are relatively straight-for...
Spatiotemporal Dynamics of Kinase Signaling Visualized by Targeted Reporters [0.03%]
基于靶向报告基因的激酶信号时空动态研究
Maya T Kunkel,Alexandra C Newton
Maya T Kunkel
The advent of genetically encoded FRET-based kinase activity reporters has ushered in a new era of signal transduction research. Such reporters allow the direct monitoring of kinase activity in live cells at specific locations, providing un...
Mass spectrometry-based identification of protein kinase substrates utilizing engineered kinases and thiophosphate labeling [0.03%]
基于质谱的蛋白质激酶底物鉴定利用工程化激酶和硫基磷酸化标记技术
Yong Chi,Bruce E Clurman
Yong Chi
Protein kinases constitute a large enzyme family with key roles in cellular signal transduction. One way to elucidate the functions of protein kinases is to systematically identify their downstream targets. We present here a simple and effe...
Activity-Based Protein Profiling (ABPP) and Click Chemistry (CC)-ABPP by MudPIT Mass Spectrometry [0.03%]
基于质谱的MudPIT分析法进行蛋白质活化谱分析(ABPP)及“点击化学”(Click Chemistry, CC)-ABPP
Anna E Speers,Benjamin F Cravatt
Anna E Speers
Activity-based protein profiling (ABPP) is a chemical proteomic method for functional interrogation of enzymes within complex proteomes. This Unit presents a protocol for in vitro and in vivo labeling using ABPP and Click Chemistry (CC)-ABP...