J Sebastian Temme,Isaac J Krauss
J Sebastian Temme
In vitro selection of nucleic acid aptamers, coined SELEX, has led to the discovery of novel therapeutics and aided in the structural and mechanistic understanding of many ligand-biomolecule interactions. A related method, selection with mo...
Kritika Mohan,Reginald M Penner,Gregory A Weiss
Kritika Mohan
Virus electrodes address two major challenges associated with biosensing. First, the surface of the viruses can be readily tailored for specific, high affinity binding to targeted biomarkers. Second, the viruses are entrapped in a conductin...
Digestion, Purification, and Enrichment of Protein Samples for Mass Spectrometry [0.03%]
蛋白质样品的消化、纯化与富集用于质谱分析
Victoria E Hedrick,Mercedes N LaLand,Ernesto S Nakayasu et al.
Victoria E Hedrick et al.
Proteomic studies rely heavily on the use of liquid chromatography (LC)-mass spectrometry (MS and MS/MS) analyses to provide information about protein composition and function. Profiling the proteome can be the first step to understanding b...
Kalyn A Brown,Alexander Deiters
Kalyn A Brown
The expansion of the genetic code of mammalian cells enables the incorporation of unnatural amino acids into proteins. This is achieved by adding components to the protein biosynthetic machinery, specifically an engineered aminoacyl-tRNA sy...
Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs) to Monitor Compound Effects on Cardiac Myocyte Signaling Pathways [0.03%]
应用人诱导多能干细胞源心脏细胞监测化合物对心肌细胞信号通路的影响
Liang Guo,Sandy Eldridge,Mike Furniss et al.
Liang Guo et al.
There is a need to develop mechanism-based assays to better inform risk of cardiotoxicity. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are rapidly gaining acceptance as a biologically relevant in vitro model for u...
Geyou Ao,Ming Zheng
Geyou Ao
Purification of single-chirality single-wall carbon nanotubes (SWCNTs) from their synthetic mixture is a prerequisite for many applications. DNA-controlled carbon nanotube (CNT) purification has evolved over a decade along with other separa...
Diffraction-unlimited fluorescence microscopy of living biological samples using pcSOFI [0.03%]
利用pcSOFI进行活体生物样品的衍射无关荧光显微scopy
Sam Duwé,Benjamien Moeyaert,Peter Dedecker
Sam Duwé
The complex microscopic nature of many live biological processes is often obscured by the diffraction limit of light, requiring diffraction-unlimited fluorescence microscopy to resolve them. Because of the vast range of different processes ...
Universal non-antibody detection of protein phosphorylation using pIMAGO [0.03%]
利用pIMAGO实现无需抗体的蛋白质磷酸化通用检测技术
Anton B Iliuk,W Andy Tao
Anton B Iliuk
This article describes methods for a new, non-antibody phosphorylation detection reagent, termed pIMAGO (phospho-imaging). This novel reagent takes advantage not only of the unique properties of the soluble nanoparticles, but also of the mu...
Xuelai Fan,Yu Tian Wang
Xuelai Fan
Rapid and reversible methods for altering the function of endogenous proteins are not only indispensable tools for probing complex biological systems, but may potentially drive the development of new therapeutics for the treatment of human ...
Miniaturized High-Throughput Fluorescent Assay for Conversion of NAD(P)H to NAD(P) [0.03%]
一种用于检测NAD(P)H转化为NAD(P)的微型高通量荧光测定法
Andrew D Napper,Sharmila Sivendran
Andrew D Napper
A miniaturized fluorescent assay is described that monitors the conversion of NADPH to NADP+. The same assay format may also be used to measure NADH to NAD+ conversion. Examples of assay development and validation results are presented to i...