Intranasal vaccine combining adenovirus and trimeric subunit protein provides superior immunity against SARS-CoV-2 Omicron variant [0.03%]
针对新冠病毒奥密克戎变异株的鼻喷新冠疫苗（结合腺病毒载体与三聚体蛋白亚单位）可提供更佳免疫防护作用
Weiqi Hong,Ping Cheng,Jingyun Yang et al.
Weiqi Hong et al.
Mucosal immunity provides efficient protection against upper-airway infections, limiting viral shedding and transmission. However, currently, no nasal spray COVID-19 vaccines are approved by WHO for global use. Here we develop a two-compone...
Bead-based approaches for increased sensitivity and multiplexing of CRISPR diagnostics [0.03%]
基于珠子的方法，提高CRISPR诊断的灵敏度和多重检测能力
Sameed M Siddiqui,Nicole L Welch,Tien G Nguyen et al.
Sameed M Siddiqui et al.
CRISPR-based diagnostics have emerged as a promising tool for fast, accurate and portable pathogen detection. There has been rapid progress in pre-amplification processes and CRISPR-related enzymes used in these approaches, but the developm...
Spatial profiling of gene editing by in situ sequencing in mice and macaques [0.03%]
通过原位测序在小鼠和恒河猴中对基因编辑的空间定位分析
Sharan Janjuha,Tatjana Haenggi,Thomas C Chamberlain et al.
Sharan Janjuha et al.
Base and prime editing technologies precisely install defined nucleotide edits in both dividing and non-dividing cells, offering potential for correcting pathogenic mutations directly in organisms. However, to fully leverage their therapeut...
Xuan Cheng,Panzhu Yao,Chongyu Jin et al.
Xuan Cheng et al.
Immunoassays using affinity binders such as antibodies and aptamers are crucial for molecular biology. However, the advancement of analytical methods based on these affinity probes is often hampered by complex operational steps that can int...
A selection pipeline for switchable aptamer beacons in broad-spectrum protein detection [0.03%]
用于广谱蛋白质检测的可切换适配子信标的筛选方法
Maxim V Berezovski
Maxim V Berezovski
Leveraging large language and vision models for knowledge extraction from large-scale image-text colonoscopy records [0.03%]
利用大规模图像文本结肠镜记录进行知识提取的语言和视觉模型的应用
Shuo Wang,Yan Zhu,Zhiwei Yang et al.
Shuo Wang et al.
The development of artificial intelligence systems for colonoscopy analysis often necessitates expert-annotated image datasets. However, limitations in dataset size and diversity impede model performance and generalization. Image-text colon...
Label-free metabolic imaging monitors the fitness of chimeric antigen receptor T cells [0.03%]
无标签代谢成像监测嵌合抗原受体T细胞的适应度
Dan L Pham,Dan Cappabianca,Matthew H Forsberg et al.
Dan L Pham et al.
Chimeric antigen receptor (CAR) T cell therapy for solid tumours is challenging because of the immunosuppressive tumour microenvironment and a complex manufacturing process. Cellular manufacturing protocols directly impact CAR T cell yield,...
Treatment of a severe vascular disease using a bespoke CRISPR-Cas9 base editor in mice [0.03%]
利用定制的CRISPR-Cas9碱基编辑器治疗小鼠重度血管疾病的研究
Christiano R R Alves,Sabyasachi Das,Vijai Krishnan et al.
Christiano R R Alves et al.
Pathogenic missense mutations in the alpha actin isotype 2 (ACTA2) gene cause multisystemic smooth muscle dysfunction syndrome (MSMDS), a genetic vasculopathy that is associated with stroke, aortic dissection and death in childhood. Here we...