A microphysiological system for screening lipid nanoparticle-mRNA complexes predicts in vivo heart transfection efficacy [0.03%]
一种用于筛选脂质纳米颗粒-mRNA复合物的微生理系统可预测体内心脏转染效率
Gabriel Neiman,Mauro W Costa,Hesong Han et al.
Gabriel Neiman et al.
Gene transfection via lipid nanoparticle (LNP)-mRNA complexes have tremendous potential for treating cardiac diseases. However, the transfection efficiency is poor and there is a lack of in vitro screening systems that predict transfection ...
Engineered human induced pluripotent stem cell models reveal altered podocytogenesis in congenital heart disease-associated SMAD2 mutations [0.03%]
利用遗传病相关SMAD2突变的诱导多能干细胞模型揭示足细胞生成异常
Rohan Bhattacharya,Tarsha Ward,Titilola D Kalejaiye et al.
Rohan Bhattacharya et al.
Clinical observations of patients with congenital heart disease carrying SMAD2 genetic variants revealed correlations with multi-organ impairments at the developmental and functional levels. Many patients with congenital heart disease prese...
Nonexpansive biodegradable matrix promotes blood vessel organoid development for neurovascular repair and functional recovery in ischaemic stroke [0.03%]
非扩张型可降解支架促进血血管器官发生及其在缺血性脑卒中后神经血管修复和功能恢复中的作用
Dongling Xiao,Yue Sun,Guanyuan Yang et al.
Dongling Xiao et al.
Tissue engineering-based vascular reconstruction represents a promising therapeutic strategy for ischaemic stroke. However, in the confined stroke cavity, conventional implants are unable to simultaneously provide swelling-resistant support...
Systematic production of human kidney organoids for transplantation in porcine kidneys during ex vivo machine perfusion [0.03%]
人肾脏类器官的系统生产及其在体外机器灌注猪肾移植中的应用
Elena Garreta,Daniel Moya-Rull,Alberto Centeno et al.
Elena Garreta et al.
Organoids derived from human pluripotent stem (hPS) cells hold promise for therapeutic purposes. However, technological advances to overcome their massive production while ensuring differentiation fidelity are still lacking. Here we report ...
Steric stabilization-independent stealth cloak enables nanoreactors-mediated starvation therapy against refractory cancer [0.03%]
基于非立体稳定隐身外罩的纳米反应器介导的抗难治性肿瘤饥饿疗法
Junjie Li,Kazuko Toh,Panyue Wen et al.
Junjie Li et al.
The high interfacial energy of nanomaterials limits their certain biomedical applications that require stealthiness to minimize non-specific interaction with biological components. While steric repulsion-based entropic stabilization-such as...
NotifAI-OS: an AI framework for automated CT-based opportunistic screening in post-acute value-based care [0.03%]
基于CT的机遇性筛查人工智能框架用于急性后期以价值为基础护理中的自动预警系统（NotifAI-OS）
Rohit Reddy,Perry J Pickhardt,Arjun Manrai et al.
Rohit Reddy et al.
High-throughput evaluation of in vitro CRISPR activities enables optimized large-scale multiplex enrichment of rare variants [0.03%]
高效的体外CRISPR筛选方法实现稀有变异体的大规模多重富集
Joo Hye Yeo,Seungho Lee,Seungmin Kim et al.
Joo Hye Yeo et al.
Previous high-throughput evaluations of CRISPR activities for a large number of target and guide RNA sequences were based on measuring insertion-deletion frequencies rather than cleavage efficiencies. Here we develop two high-throughput in ...