In vivo volumetric visualization and quantification of cumulus expansion in mice with intravital optical coherence tomography [0.03%]
活体光学相干层析在小鼠体内可视化和量化卵子颗粒扩展状况
Kohei Umezu,Frank J Brooks,Irina Larina
Kohei Umezu
Ovulation is preceded by a critical physiological process known as cumulus expansion, during which the cumulus cell layer surrounding the oocyte undergoes structural remodeling. Despite the recognized importance of this process for reproduc...
Dynamic optical coherence tomography algorithm for label-free assessment of swiftness and occupancy of intratissue moving scatterers [0.03%]
用于评估组织内移动散射物速度和占用率的动态光学相干层析成像算法
Rion Morishita,Pradipta Mukherjee,Ibrahim Abd El-Sadek et al.
Rion Morishita et al.
Dynamic optical coherence tomography (DOCT) statistically analyzes fluctuations in time-sequential OCT signals, enabling label-free and three-dimensional visualization of intratissue and intracellular activities. Current DOCT methods, such ...
Cardiac pulse transfer along the cerebral microvascular network captured by laser speckle contrast imaging [0.03%]
基于激光散斑对比度成像的脑微血管网络中心脏脉冲传输的动力学特征研究
Rasmus Juul Jensen,Mia Viuf Skøtt,Dmitry D Postnov
Rasmus Juul Jensen
Detecting subtle alterations in cardiac pulse transmission through the cerebral microvascular network could provide a new avenue for characterising cerebral microvascular health and aid in the research and diagnosis of related neurological ...
Maximising imaging volumes of expanded tissues for inverted fluorescence microscopy [0.03%]
用于倒置荧光显微镜的展开组织的最大化成像体积
Miguel Cardoso Mestre,Jacob R Lamb,Madeline A Lancaster et al.
Miguel Cardoso Mestre et al.
Expansion microscopy (ExM) has enabled nanoscale imaging of tissues by physically enlarging biological samples in a swellable hydrogel. However, the increased sample size and water-based environment pose challenges for deep imaging using co...
Development of a laser speckle contrast imaging analysis pipeline reveals a triphasic retinal blood flow waveform [0.03%]
激光散斑对比成像分析管道的开发揭示了视网膜血流三相波形
Alexander J Tate,Gary C Mouradian Jr,Daniel M Lipinski
Alexander J Tate
Laser speckle contrast imaging (LSCI) is an under-utilized retinal imaging modality capable of non-invasively generating contrast-free, widefield maps of retinal blood flow from which multiple metrics of retinal hemodynamic function can be ...
Miniaturized widefield microscope for high speed in vivo voltage imaging [0.03%]
一种用于在体高速电压成像的微型宽场显微镜
Catherine A Saladrigas,Forest Speed,Alec Teel et al.
Catherine A Saladrigas et al.
Functional imaging in freely moving animals with genetically encoded voltage indicators (GEVIs) will open new capabilities for neuroscientists to study the behavioral relevance of neural activity with high spatial and temporal precision. Ho...
Neha Goswami,Mark A Anastasio
Neha Goswami
The space-bandwidth product (SBP) imposes a fundamental limitation in achieving high-resolution and large field-of-view image acquisitions simultaneously. High-NA objectives provide fine structural detail at the cost of reduced spatial cove...
Xinyu Wang,Silvestre Manzanera,Juan Tabernero et al.
Xinyu Wang et al.
Pulsed IR light can be perceived as visible through a non-linear two-photon (2P) absorption process. Similar to visible light, the Stiles-Crawford effect of the first kind (SCE-I) predicts that the perceived brightness in 2P vision depends ...
Lung cancer screening based on plasma-derived exosomes via droplet coating deposition Raman spectroscopy and machine learning [0.03%]
基于droplet coating deposition拉曼光谱和机器学习的血浆衍生外泌体肺癌筛查技术
Yuyi Li,Shuting Xiao,Jing Huang et al.
Yuyi Li et al.
Lung cancer is the leading cause of cancer-related mortality worldwide, making early screening crucial for improving patient survival. In recent years, exosomes have garnered significant attention as promising biomarkers for the detection o...
Label-free viability assessment of ex vivo mouse kidneys for transplant applications using dynamic optical coherence tomography [0.03%]
基于动态光学相干层析的无标记活体小鼠肾脏移植适配性评估方法
Ke Zhang,Qinghao Zhang,Trisha Valerio et al.
Ke Zhang et al.
Kidney transplantation remains the preferred treatment for patients with end-stage kidney disease. However, the ongoing shortage of donor organs continues to limit the availability of transplant treatments. Existing evaluation methods, such...