Phosphirenium Ions as Masked Phosphenium Catalysts: Mechanistic Evaluation and Application in Synthesis [0.03%]
作为掩蔽的磷叶啉催化剂的膦𬭩离子：机理评估及在合成中的应用
Danila Gasperini,Samuel E Neale,Mary F Mahon et al.
Danila Gasperini et al.
The utilization of phosphirenium ions is presented; optimized and broadened three-membered ring construction is described together with the use of these ions as efficient pre-catalysts for metal-free carbonyl reduction with silanes. Full ch...
Accessing Chemo- and Regioselective Benzylic and Aromatic Oxidations by Protein Engineering of an Unspecific Peroxygenase [0.03%]
通过过氧化酶的蛋白质工程实现化学选择性和区域选择性的苄位和芳香氧化反应
Anja Knorrscheidt,Jordi Soler,Nicole Hünecke et al.
Anja Knorrscheidt et al.
Unspecific peroxygenases (UPOs) enable oxyfunctionalizations of a broad substrate range with unparalleled activities. Tailoring these enzymes for chemo- and regioselective transformations represents a grand challenge due to the difficulties...
Enantioselective Inverse-Electron Demand Aza-Diels-Alder Reaction: ipso,α-Selectivity of Silyl Dienol Ethers [0.03%]
反电子需求氮杂Diels-Alder反应的对映选择性及硅基二烯醇醚的ipso，α-选择性
Víctor Laina-Martín,Jorge Humbrías-Martín,Rubén Mas-Ballesté et al.
Víctor Laina-Martín et al.
A highly efficient enantioselective inverse-electron-demand aza-Diels-Alder reaction between aza-sulfonyl-1-aza-1,3-butadienes and silyl (di)enol ethers has been developed. The presented methodology allows the synthesis of benzofuran-fused ...
The Peptide Ligase Activity of Human Legumain Depends on Fold Stabilization and Balanced Substrate Affinities [0.03%]
人源Legumain肽类连接酶活性依赖于构象稳定及平衡底物亲合力
Elfriede Dall,Vesna Stanojlovic,Fatih Demir et al.
Elfriede Dall et al.
Protein modification by enzymatic breaking and forming of peptide bonds significantly expands the repertoire of genetically encoded protein sequences. The dual protease-ligase legumain exerts the two opposing activities within a single prot...
Synthesis of γ-Hydroxy-α-amino Acid Derivatives by Enzymatic Tandem Aldol Addition-Transamination Reactions [0.03%]
通过酶催化连续的醛醇加成-转氨反应合成γ-羟基-α-氨基酸衍生物
Carlos J Moreno,Karel Hernández,Simon J Charnok et al.
Carlos J Moreno et al.
Three enzymatic routes toward γ-hydroxy-α-amino acids by tandem aldol addition-transamination one-pot two-step reactions are reported. The approaches feature an enantioselective aldol addition of pyruvate to various nonaromatic aldehydes ...
Kinetic Characterization of a Putatively Chitin-Active LPMO Reveals a Preference for Soluble Substrates and Absence of Monooxygenase Activity [0.03%]
具有假定的几丁质活性的LPMO的动力学特征表明其对可溶底物的偏好和单加氧酶活性的缺失
Lukas Rieder,Dejan Petrović,Priit Väljamäe et al.
Lukas Rieder et al.
Enzymes known as lytic polysaccharide monooxygenases (LPMOs) are recognized as important contributors to aerobic enzymatic degradation of recalcitrant polysaccharides such as chitin and cellulose. LPMOs are remarkably abundant in nature, wi...
What Does the Brønsted Slope Measure in the Phosphoryl Transfer Transition State? [0.03%]
Brønsted 斜率在磷酸转移过渡态中测量什么？
Rui Lai,Qiang Cui
Rui Lai
The structural and energetic features of phosphate and phosphonate hydrolysis in Protein Phosphatase-1 (PP1) and water are studied using quantum mechanical (QM) cluster models. The calculations are able to reproduce observed kinetic isotope...
Recent Advances in Homogeneous Catalysts for the Asymmetric Hydrogenation of Heteroarenes [0.03%]
杂芳烃不对称氢化的均相催化剂的研究进展
Alexia N Kim,Brian M Stoltz
Alexia N Kim
The asymmetric hydrogenation of heteroarenes has recently emerged as an effective strategy for the direct access to enantioenriched, saturated heterocycles. Although several homogeneous catalyst systems have been extensively developed for t...
A Tailor-Made Deazaflavin-Mediated Recycling System for Artificial Nicotinamide Cofactor Biomimetics [0.03%]
用于烟酰胺辅因子生物模拟物的定制化脱氮黄素介导循环系统
Jeroen Drenth,Guang Yang,Caroline E Paul et al.
Jeroen Drenth et al.
Nicotinamide adenine dinucleotide (NAD) and its 2'-phosphorylated form NADP are crucial cofactors for a large array of biocatalytically important redox enzymes. Their high cost and relatively poor stability, however, make them less attracti...
Rigidifying a De Novo Enzyme Increases Activity and Induces a Negative Activation Heat Capacity [0.03%]
增强从头酶的刚性可以提高活性并引发负活化热容量
Sarah A Hindson,H Adrian Bunzel,Bettina Frank et al.
Sarah A Hindson et al.
Conformational sampling profoundly impacts the overall activity and temperature dependence of enzymes. Peroxidases have emerged as versatile platforms for high-value biocatalysis owing to their broad palette of potential biotransformations....