In Silico Identification and Experimental Validation of Distal Activity-Enhancing Mutations in Tryptophan Synthase [0.03%]
色氨酸合成酶中远端活性增强突变的计算机识别与实验验证
Miguel A Maria-Solano,Thomas Kinateder,Javier Iglesias-Fernández et al.
Miguel A Maria-Solano et al.
Allostery is a central mechanism for the regulation of multi-enzyme complexes. The mechanistic basis that drives allosteric regulation is poorly understood but harbors key information for enzyme engineering. In the present study, we focus o...
Does the Configuration at the Metal Matter in Noyori-Ikariya Type Asymmetric Transfer Hydrogenation Catalysts? [0.03%]
金属处的构型在Noyori-Ikariya类型不对称转移氢化催化剂中是否有影响？
Andrew M R Hall,Daniel B G Berry,Jaime N Crossley et al.
Andrew M R Hall et al.
Noyori-Ikariya type [(arene)RuCl(TsDPEN)] (TsDPEN, sulfonated diphenyl ethylenediamine) complexes are widely used C=O and C=N reduction catalysts that produce chiral alcohols and amines via a key ruthenium-hydride intermediate that determin...
Processive Enzymes Kept on a Leash: How Cellulase Activity in Multienzyme Complexes Directs Nanoscale Deconstruction of Cellulose [0.03%]
拴在 leash 上的连续酶：多酶复合物中的纤维素酶活性如何指导纳米级的纤维素拆解
Krisztina Zajki-Zechmeister,Gaurav Singh Kaira,Manuel Eibinger et al.
Krisztina Zajki-Zechmeister et al.
Biological deconstruction of polymer materials gains efficiency from the spatiotemporally coordinated action of enzymes with synergetic function in polymer chain depolymerization. To perpetuate enzyme synergy on a solid substrate undergoing...
GaPt Supported Catalytically Active Liquid Metal Solution Catalysis for Propane Dehydrogenation-Support Influence and Coking Studies [0.03%]
用于丙烷脱氢的负载液态金属催化溶液催化-载体影响和积炭研究
Narayanan Raman,Moritz Wolf,Martina Heller et al.
Narayanan Raman et al.
Supported catalytically active liquid metal solutions (SCALMS) of Pt in Ga (2 at.-% Pt) were studied in the temperature range of 500 to 600 °C for propane dehydrogenation. A facile synthesis procedure using ultrasonication was implemented ...
Flexibility Enhances Reactivity: Redox Isomerism and Jahn-Teller Effects in a Bioinspired Mn4O4 Cubane Water Oxidation Catalyst [0.03%]
柔性增强反应性：受生物启发的Mn4O4立方烷水氧化催化剂中的氧化还原异构化和詹-泰勒效应
Ludwig Schwiedrzik,Vera Brieskorn,Leticia González
Ludwig Schwiedrzik
Understanding how water oxidation to molecular oxygen proceeds in molecular metal-oxo catalysts is a challenging endeavor due to their structural complexity. In this report, we unravel the water oxidation mechanism of the highly active wate...
Srinivas Kolluru,Manvendra Singh,Bryce Gaskins et al.
Srinivas Kolluru et al.
We report the discovery, development, and mechanism of a nickel-catalyzed annulation reaction between o-haloarylimines and electron-poor olefins. The reaction produces two adjacent anti stereocenters and a free secondary amine. Spirocycles ...
GH47 and Other Glycoside Hydrolases Catalyze Glycosidic Bond Cleavage with the Assistance of Substrate Super-arming at the Transition State [0.03%]
GH47和其他糖苷水解酶在底物过渡态超级激活的帮助下催化糖苷键的断裂
Jonathan C K Quirke,David Crich
Jonathan C K Quirke
Super-armed glycosyl donors, whose substituents are predominantly held in pseudoaxial positions, exhibit strongly increased reactivity in glycosylation through significant stabilization of oxocarbenium-like transition states. Examination of...
A High-Throughput Method for Directed Evolution of NAD(P)+-Dependent Dehydrogenases for the Reduction of Biomimetic Nicotinamide Analogues [0.03%]
用于生物模拟烟酰胺类似物还原的NAD（P）+依赖性脱氢酶定向进化的高通量方法
Rui Huang,Hui Chen,David M Upp et al.
Rui Huang et al.
Engineering flavin-free NAD(P)+-dependent dehydrogenases to reduce biomimetic nicotinamide analogues (mNAD+s) is of importance for eliminating the need for costly NAD(P)+ in coenzyme regeneration systems. Current redox dye-based screening m...
Modular Enantioselective Synthesis of cis-Cyclopropanes through Self-Sensitized Stereoselective Photodecarboxylation with Benzothiazolines [0.03%]
通过苯并噻唑啉的自敏立体选择性光脱羧反应模块化对映选择性合成cis-环丙烷
Matteo Costantini,Abraham Mendoza
Matteo Costantini
Chiral cis-cyclopropanes are strained rigid analogues of alkyl chains, whose study and application are limited by their difficult synthesis. A modular approach from olefin materials is enabled by the discovery of the electron donor-acceptor...
Unlocking Asymmetric Michael Additions in an Archetypical Class I Aldolase by Directed Evolution [0.03%]
通过定向进化在一类典型的I型醛缩酶中实现不对称迈克尔加成反应
Andreas Kunzendorf,Guangcai Xu,Jesse J H van der Velde et al.
Andreas Kunzendorf et al.
Class I aldolases catalyze asymmetric aldol addition reactions and have found extensive application in the biocatalytic synthesis of chiral β-hydroxy-carbonyl compounds. However, the usefulness of these powerful enzymes for application in ...