Isoform-resolved transcriptomics reveals stage-specific isoform switching and protein domain remodeling in Helicoverpa armigera [0.03%]
转录组揭示棉铃虫发育阶段特异性异构体转换及蛋白质结构域重塑
Himalshi K Wickramage,Jack W Royle,Pawel Sadowski et al.
Himalshi K Wickramage et al.
Insects exhibit pronounced developmental plasticity, driven by complex regulation of gene expression, including isoform-level modulation across developmental stages. However, the role of isoform-level regulation in the developmental transit...
Promoter contacts by TFB and TFEα during archaeal transcription initiation [0.03%]
TFB和TFEα在古菌转录起始过程中与启动子的相互作用
Michael Micorescu,Kimberly Sheffield,Vlad Geshka et al.
Michael Micorescu et al.
Transcription initiation by archaeal RNA polymerase (RNAP) is assisted by transcription factors, TBP, TFB, and TFEα, which show sequence, structural and functional homology to eukaryotic TBP, TFIIB, and TFIIEα. Previous low-resolution cro...
Rebecca L Skalsky
Rebecca L Skalsky
MicroRNAs (miRNAs) are small, noncoding RNAs that have emerged as powerful players in the post-transcriptional regulation of gene expression. In the context of viral infections, cellular miRNAs play significant roles in modulating the compl...
Endogenous eukaryotic CHO-K1 cell promoters as tools to improve the production of a difficult-to-express recombinant protein [0.03%]
内源性真核CHO-K1细胞启动子作为改善难以表达的重组蛋白生产的工具
María Belén Ardusso,Agustina Gugliotta,Victoria Gastaldi et al.
María Belén Ardusso et al.
Recombinant proteins with applications in human and veterinary medicine are mainly produced in mammalian expression systems, particularly Chinese hamster ovary (CHO) cells. Although these cells enable proper folding and post-translational m...
The Goldilocks state of transcriptional readthrough for chimeric splicing [0.03%]
转录读码框延伸的“金发姑娘状态”与嵌合剪接事件的关系
Guillaume F Chanfreau
Guillaume F Chanfreau
Proper transcription termination coupled to pre-mRNA cleavage and polyadenylation is an important step in gene expression as it generates the 3'UTR of mRNAs and defines transcription units. Defective termination (transcriptional readthrough...
XAB2: a link between RNA metabolism, DNA damage repair, and human health [0.03%]
XAB2：RNA代谢、DNA损伤修复和人类健康之间的联系
Lucia Borszekova Pulzova,Miroslav Sabo,Miroslav Chovanec
Lucia Borszekova Pulzova
Cells have evolved multiple mechanisms to preserve genome integrity, collectively known as DNA damage response (DDR). Rather than acting separately, the DDR often interacts with transcription and mRNA splicing; however, the underlying molec...
miRNA dynamics in development: orchestrators of gene expression and cell fate [0.03%]
微小核糖核酸在发育中的动态变化：基因表达和细胞命运的调控者
Vaishnav Vasudevan,Mathieu W Segonds,Martin J Simard
Vaishnav Vasudevan
MicroRNAs (miRNAs) are central regulators of gene expression, shaping cell fate, tissue identity, and organismal physiology. From the earliest stages of development through later processes of tissue differentiation, miRNAs operate within dy...
Genomic and structural insights into TATA-Binding protein from cestodes [0.03%]
cestode TATA结合蛋白的基因组和结构洞察
Abraham Landa,Elisa Heredia-Gómez,Cristina González-Kulikova et al.
Abraham Landa et al.
The TATA-binding protein (TBP) is an essential component of the eukaryotic transcriptional machinery, yet little is known about the structure, evolution, and functional divergence of TBP paralogs in parasitic flatworms. Here, we performed a...
ADAR-mediated tolerance and SOS splicing-mediated excision of transposable elements [0.03%]
由ADAR介导的容受性和SOS剪接介导的转座子切除反应
Qi Cao,Yuange Duan
Qi Cao
Transposable elements (TEs) threaten genomic integrity, yet their pervasive presence indicates the limitations of existing silencing mechanisms. A recent paper in Nature (Zhao et al. 2025) discovered the SOS splicing system, which provides ...
Structure-to-function relationships of RNA polymerases: recent advances in bacterial transcription termination [0.03%]
RNA聚合酶的结构与功能关系：细菌转录终止领域的最新进展
Shuang Wang
Shuang Wang
Transcription termination is a highly regulated step which sets boundaries between genes and maintains genome integrity. Defects in transcription termination will cause unexpected expression of downstream genes and traffic-jam of RNA polyme...