In vitro and in vivo characterization of putative porcine embryonic stem cells [0.03%]
猪胚胎干细胞的体外和体内特征表征
Ivan Vassiliev,Svetlana Vassilieva,Luke F S Beebe et al.
Ivan Vassiliev et al.
We have developed a new method for the isolation of porcine embryonic stem cells (ESCs) from in vivo-derived and in vitro-produced embryos. Here we describe the isolation and characterization of several ESC lines established using this meth...
Aberrant expression and methylation status of putatively imprinted genes in placenta of cloned piglets [0.03%]
克隆猪胎盘中疑似印记基因的异常表达及甲基化状态研究
Yanchang Wei,Jiang Zhu,Yanjun Huan et al.
Yanchang Wei et al.
Unlike embryos derived from fertilization, most cloned embryos die during postimplantation development, and those that survive to term are frequently defective. Many of the observed defects involve placenta. Abnormal placentation has been d...
Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes [0.03%]
高效地从冷冻卵子的核移植和孤雌胚胎中分离胚胎干细胞
Li-Ying Sung,Ching-Chien Chang,Tomokazu Amano et al.
Li-Ying Sung et al.
Deriving histocompatible embryonic stem (ES) cells by somatic cell nuclear transfer (SCNT) and parthenogenetic activation (PA) requires fresh oocytes, which prevents their applications in humans. Here, we evaluated the efficiency of derivin...
Isabelle Cassar-Malek,Brigitte Picard,Catherine Jurie et al.
Isabelle Cassar-Malek et al.
We have recently reported that maturation of the skeletal muscle is delayed in cloned calves during their first year postnatally. This delay could originate from perturbations in fetal myogenesis. The aim of this study was to evaluate the d...
Effect of human chorionic gonadotropin and progesterone administration on the developmental potential of mouse somatic cell nuclear-transferred oocytes [0.03%]
人绒毛膜促性腺激素和孕酮对小鼠体细胞核移植重构胚发育潜能的影响
Yuta Tsuji,Yoko Kato,Yukio Tsunoda
Yuta Tsuji
Somatic cell nuclear-transferred (SCNT) oocytes have a relatively high potential to develop into blastocysts in vitro, but a large proportion embryos die at various pre- and postimplantation stages after transfer to recipients. Although the...
Quantification of leukocyte genomic 5-methylcytosine levels reveals epigenetic plasticity in healthy adult cloned cattle [0.03%]
定量测定白细胞基因组5-胞嘧啶甲基化水平揭示健康成年克隆牛的表观遗传塑性
Béatrice de Montera,Dalia El Zeihery,Sigrid Müller et al.
Béatrice de Montera et al.
Successful somatic cell nuclear transfer (SCNT) requires epigenetic reprogramming of a differentiated donor cell nucleus. Incorrect reprogramming of epigenetic markings such as DNA methylation is associated with compromised prenatal develop...
Guochun Gong,Marsha L Roach,Le Jiang et al.
Guochun Gong et al.
The goals of the current study were to (1) improve culture conditions and (2) chemical passaging of bovine embryonic stem cell-like (bESC-like) cells. Specifically, the effects of human leukemia inhibitory factor (hLIF), two types of feeder...
Growth and hematologic characteristics of cloned dogs derived from adult somatic cell nuclear transfer [0.03%]
由体细胞核移植克隆的成年犬的生长及血液学特征研究
Jung Eun Park,Min Kyu Kim,Jung Taek Kang et al.
Jung Eun Park et al.
Three viable female dogs, which have the same genotype, have been successfully produced by somatic cell nuclear transfer (SCNT); however, data on the growth pattern of cloned dogs are lacking. Thus, the aim of this study was (1) to assess g...
The comparison between conditioned media and serum-free media in human embryonic stem cell culture and differentiation [0.03%]
人胚胎干细胞培养及分化的条件培养基与无血清培养基的比较
Zara Hannoun,Judy Fletcher,Sebastian Greenhough et al.
Zara Hannoun et al.
Human embryonic stem cells (hESCs) offer an inexhaustible supply of human somatic cell types through their ability to self-renew while retaining pluripotency. As such, hESC-derived cell types are important for applications ranging from in v...
Comparative Study
Cellular reprogramming. 2010 Apr;12(2):133-40. DOI:10.1089/cell.2009.0099 2010
Latrunculin A dramatically improves the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells [0.03%]
_latrunculin A显著改善了来自基因修饰克隆迷你猪细胞的核移植胚胎的发育能力_
Takehiro Himaki,Hironori Mori,Yamato Mizobe et al.
Takehiro Himaki et al.
This study was carried out to examine the effect of postactivation treatment with latrunculin A (LatA), an actin polymerisation inhibitor, on in vitro and in vivo development of somatic cell nuclear transfer (SCNT) embryos derived from gene...