The efficacy of human placenta as a source of the universal feeder in human and mouse pluripotent stem cell culture [0.03%]
胎盘作为人类和小鼠多能干细胞培养的通用饲养层细胞源的有效性研究
Yong Park,Seung Jin Lee,In Young Choi et al.
Yong Park et al.
The use of a mouse embryonic fibroblast (MEF) feeder for culture of embryonic stem cells (ESCs) is a widely accepted method, regardless of the ESCs' origin and type. In this study, we performed the undifferentiated propagation of human ES c...
Optimization of culture conditions to support undifferentiated growth of human embryonic stem cells [0.03%]
优化培养条件以促进人胚胎干细胞的未分化增殖
Dongmei Lai,Weiwei Cheng,Te Liu et al.
Dongmei Lai et al.
Human embryonic stem cells (hESCs) are usually maintained in an undifferentiated state by coculture with mitomycin C-treated mouse embryonic fibroblasts (MEFs) as feeder cells in the presence of animal sera such as fetal bovine serum (FBS)....
Human fetal trophonema matrix and uterine endometrium support better human embryonic stem cell growth and neural differentiation than mouse embryonic fibroblasts [0.03%]
人胎盘基质和子宫内膜细胞比小鼠胚胎成纤维细胞更好地支持人体干细胞的生长及神经分化
Xuan Gao,Junhao Yan,Yun Shen et al.
Xuan Gao et al.
The concerns over xenogeneic pathogens and immunogenic molecules derived from mouse embryonic fibroblasts (MEFs) trigger the development of human-derived feeder layers for human embryonic stem cell (hESC) maintenance. It is essential to eva...
Acclimatized induction reveals the multipotency of adult human undifferentiated keratinocytes [0.03%]
适应性诱导揭示成人人类未分化角质形成细胞的多能性
Weidong Han,Meixia Chen,Meirong Li et al.
Weidong Han et al.
It has been demonstrated that several types of somatic stem cells have the remarkable capacity to differentiate into other types of tissues. However, the promise of keratinocyte stem cells seems slim for generating nonepidermal tissues. Usi...
Optimization of genetic engineering and homologous recombination of collagen type I genes in rat bone marrow mesenchymal stem cells (MSC) [0.03%]
优化胶原蛋白基因I在 rats 骨髓间充质干细胞中的同源重组及基因治疗研究
Maciej Tarnowski,Anna Szydło,Jacek Anioł et al.
Maciej Tarnowski et al.
Mutations in COL1A1 or COL1A2 genes lead to osteogenesis Imperfecta (OI) in humans. There are three possiblities to successfully treat OI including (1) gene therapy, (2) mesenchymal stem cell (MSC) therapy, or (3) a combination of both. The...
mTOR-mediated activation of p70 S6K induces differentiation of pluripotent human embryonic stem cells [0.03%]
哺乳动物雷帕霉素靶蛋白介导的p70S6激酶活化诱导多能性人类胚胎干细胞分化
Charles A Easley th,Ahmi Ben-Yehudah,Carrie J Redinger et al.
Charles A Easley th et al.
Deciding to exit pluripotency and undergo differentiation is of singular importance for pluripotent cells, including embryonic stem cells (ESCs). The molecular mechanisms for these decisions to differentiate, as well as reversing those deci...
Induction of mesenchymal progenitor cells with chondrogenic property from mouse-induced pluripotent stem cells [0.03%]
源自诱导多能干细胞的具有软骨分化潜能的间充质祖细胞的诱导
Takeshi Teramura,Yuta Onodera,Toshihiro Mihara et al.
Takeshi Teramura et al.
Despite recent cell-engineering advances, treatment and repair of cartilage remains challenging. Although stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a prominent strategy, the major problem of limited...
Generation of human-induced pluripotent stem cells from gut mesentery-derived cells by ectopic expression of OCT4/SOX2/NANOG [0.03%]
通过异位表达OCT4/SOX2/NANOG由肠系膜细胞诱导生成人类多能干细胞
Yang Li,Hongxi Zhao,Feng Lan et al.
Yang Li et al.
Induced pluripotent stem (iPS) cells have been generated from human somatic cells by ectopic expression of defined transcription factors. Application of this approach in human cells may have enormous potential to generate patient-specific p...
Xenooplasmic transfer between buffalo and bovine enables development of homoplasmic offspring [0.03%]
水 buffalo 和牛之间的异种细胞质移植可产生均一氧化型子代
Marcos Roberto Chiaratti,Christina Ramires Ferreira,Flávio Vieira Meirelles et al.
Marcos Roberto Chiaratti et al.
Nuclear-mitochondrial incompatibilities may be responsible for the development failure reported in embryos and fetuses produced by interspecies somatic cell nuclear transfer (iSCNT). Herein we performed xenooplasmic transfer (XOT) by introd...
Porcine skin-derived progenitor (SKP) spheres and neurospheres: Distinct "stemness" identified by microarray analysis [0.03%]
微阵列分析鉴定的猪皮肤源性前体(SKP)小球和神经小球具有不同的“干细胞”特性
Ming-Tao Zhao,Kristin M Whitworth,Hui Lin et al.
Ming-Tao Zhao et al.
Skin-derived progenitors (SKP) are neural crest derived and can generate neural and mesodermal progeny in vitro, corresponding to the multipotency of neural crest stem cells. Likewise, neural stem/progenitor cells (displaying as neurosphere...