Hand-made cloned goat (Capra hircus) embryos—a comparison of different donor cells and culture systems [0.03%]
不同供体细胞和培养体系对山羊（Capra hircus）胚胎发育的影响
Yogesh S Akshey,Dhruba Malakar,Arun K De et al.
Yogesh S Akshey et al.
Nuclear transfer is a very effective method for propagation of valuable, extinct, and endangered animals. Hand-made cloning (HMC) is an efficient alternative to the conventional micromanipulator-based technique in some domestic species. The...
Comparative Study
Cellular reprogramming. 2010 Oct;12(5):581-8. DOI:10.1089/cell.2009.0120 2010
Epigenetic differences between embryonic stem cells generated from blastocysts developed in vitro and in vivo [0.03%]
体内和体外培养的囊胚衍生的胚胎干细胞之间的表观遗传学差异
Takuro Horii,Eikichi Yanagisawa,Mika Kimura et al.
Takuro Horii et al.
Embryonic stem (ES) cells constitute a very important tool for regenerative medicine today. These ES cells, and human ES cells in particular, are almost all derived from embryos obtained by in vitro fertilization (IVF) and from in vitro cul...
Comparative Study
Cellular reprogramming. 2010 Oct;12(5):551-63. DOI:10.1089/cell.2009.0104 2010
Confirmation of germ-line transmission in the red fluorescence protein (RFP) transgenic cloned male cat [0.03%]
红色荧光蛋白(RFP)转基因克隆猫系的生殖细胞传递确认
Su-Jin Cho,Young S Lee,Jae-Ik Lee et al.
Su-Jin Cho et al.
The production of transgenic animals is highly desirable for biotechnology and basic research. We investigated the reproductive ability of a red fluorescence protein (RFP) transgenic cloned male cat (RFP TG cat) by natural mating with a dom...
Adenovirus vector-mediated efficient transduction into human embryonic and induced pluripotent stem cells [0.03%]
腺病毒载体介导的人胚胎和诱导多能干细胞的有效转导
Katsuhisa Tashiro,Kenji Kawabata,Mitsuru Inamura et al.
Katsuhisa Tashiro et al.
We examined the transduction efficiency in human embryonic stem (ES) and induced pluripotent stem (iPS) cells using an adenovirus (Ad) vector. RT-PCR analysis revealed the expression of the coxsackievirus and adenovirus receptor, a receptor...
Aberrant expression patterns of genes involved in segregation of inner cell mass and trophectoderm lineages in bovine embryos derived from somatic cell nuclear transfer [0.03%]
细胞核移植胚胎中与内细胞团和滋养层分化相关的基因异常表达模式分析
Takashi Fujii,Satoru Moriyasu,Hiroki Hirayama et al.
Takashi Fujii et al.
High rates of embryonic, fetal, or placental abnormalities have consistently been observed in bovine cloning. Segregation of inner cell mass (ICM) and trophectoderm (TE) lineages in early embryos is an important process for fetal and placen...
Comparative Study
Cellular reprogramming. 2010 Oct;12(5):617-25. DOI:10.1089/cell.2010.0017 2010
Altered gene expression profiles in the brain, kidney, and lung of deceased neonatal cloned pigs [0.03%]
克隆仔猪供体脑、肾脏和肺的基因表达谱改变
Joonghoon Park,Sadie L Marjani,Liangxue Lai et al.
Joonghoon Park et al.
Limited studies have been published analyzing the gene expression patterns of cloned pigs. We compared the expression profiles of brain, kidney, and lung tissues, representing each of the three germ layers, of deceased neonatal cloned pigs ...
Activation with ionomycin followed by dehydroleucodine and cytochalasin B for the production of parthenogenetic and cloned bovine embryos [0.03%]
伊维菌素、dehydroleucodine和细胞松弛素B激活结合生产牛孤雌和克隆胚胎
Natalia Canel,Romina Bevacqua,Rafael Fernández-Martín et al.
Natalia Canel et al.
In this work, Dehydroleucodine (DhL) was evaluated as a chemical activator of bovine oocytes and somatic cell nuclear transfer (SCNT) reconstituted embryos. Oocytes were activated with 5 microM Ionomycin (Io) and exposed for 3 h to 1 or 5 m...
In vitro development and chromosomal configuration of bovine somatic cloned embryos with nonenucleated metaphase II oocytes [0.03%]
用元核MII卵母细胞克隆胚胎的体外发育及其染色体配置研究
Qinggang Meng,Chunling Bai,Ying Liu et al.
Qinggang Meng et al.
This study was designed to examine the effects of the presence of oocyte nuclei on the donor cell nuclear remodeling, including premature chromosome condensation (PCC) and DNA configuration, and subsequent embryo development. The results sh...
High hydrostatic pressure treatment of porcine oocytes induces parthenogenetic activation [0.03%]
高压处理诱导猪卵母细胞孤雌激活
Lin Lin,Csaba Pribenszky,Miklós Molnár et al.
Lin Lin et al.
An innovative technique called high hydrostatic pressure (HHP) treatment has recently been reported to improve the cryosurvival of gametes and embryos in certain mammalian species, including the mouse, pig, and cattle. In the present study ...
Anomalous oxygen consumption in porcine somatic cell nuclear transfer embryos [0.03%]
猪体细胞克隆胚胎的氧消耗异常现象
Satoshi Sugimura,Masaki Yokoo,Ken-ichi Yamanaka et al.
Satoshi Sugimura et al.
Oxygen consumption reflects overall metabolic activity of mammalian embryos. We measured oxygen consumption in individual porcine somatic cell nuclear transfer (SCNT) and in vitro-fertilized (IVF) embryos by modified scanning electrochemica...