Development of a model of sacrocaudal spinal cord injury in cloned Yucatan minipigs for cellular transplantation research [0.03%]
用于细胞移植研究的克隆约克郡小猪尾椎脊髓损伤模型的建立
Ji-Hey Lim,Jorge A Piedrahita,Lauren Jackson et al.
Ji-Hey Lim et al.
Research into transplantation strategies to treat spinal cord injury (SCI) is frequently performed in rodents, but translation of results to clinical patients can be poor and a large mammalian model of severe SCI is needed. The pig has been...
NKX6.1 promotes PDX-1-induced liver to pancreatic β-cells reprogramming [0.03%]
NKX6.1促进PDX-1诱导的肝向胰岛β细胞重编程
Shiraz Gefen-Halevi,Itzhak H Rachmut,Kfir Molakandov et al.
Shiraz Gefen-Halevi et al.
Reprogramming adult mammalian cells is an attractive approach for generating cell-based therapies for degenerative diseases, such as diabetes. Adult human liver cells exhibit a high level of developmental plasticity and have been suggested ...
Ezh2 expression in astrocytes induces their dedifferentiation toward neural stem cells [0.03%]
巢氏星形胶质细胞中EZH2的表达诱导其去分化为神经干细胞
Falak Sher,Erik Boddeke,Sjef Copray
Falak Sher
Recently, we have demonstrated the expression of the polycomb group protein Ezh2 in embryonic and adult neural stem cells. Although Ezh2 remained highly expressed when neural stem cells differentiate into oligodendrocyte precursor cells, it...
Effect of different culture conditions on establishment of embryonic stem cells from BALB/cAJ and NZB/BINJ mice [0.03%]
不同培养条件对BALB/cAJ和NZB/BINJ小鼠胚胎干细胞建立的影响
Saori Iijima,Yoko Tanimoto,Seiya Mizuno et al.
Saori Iijima et al.
As the phenotype of a given single-gene mutation in mice is modulated by the genetic background of the inbred strain, embryonic stem (ES) cells derived from various inbred mouse strains are required to produce gene-targeted mice without the...
Housekeeping gene transcript abundance in bovine fertilized and cloned embryos [0.03%]
牛受精和克隆胚胎管家基因转录丰度的测定
Pablo J Ross,Kai Wang,Arif Kocabas et al.
Pablo J Ross et al.
The objective of this study was to compare housekeeping gene expression levels, relative to total mRNA, across different stages of bovine preimplantation development in embryos generated by IVF and somatic cell nuclear transfer (SCNT). We f...
Nuclear transfer-specific defects are not apparent during the second week of embryogenesis in cattle [0.03%]
核移植的特异性缺陷在牛胚胎发育第二周时未显现
Craig S Smith,Debra K Berg,Martin Berg et al.
Craig S Smith et al.
Somatic cell nuclear transfer (NT)-specific effects on postblastocyst early cattle embryogenesis were investigated by comparison to in vitro-produced (IVP) embryos grown under identical conditions to embryonic days (E) 14 and 15. Recipient ...
Effect of microinjection of a single IVF-derived blastomere on the development of cloned embryos at the eight-cell stage in bovine [0.03%]
单个体外受精细胞微注射对牛八细胞阶段克隆胚胎发育的影响
Xiuping Chen,Jianwu Wang,Rong Li et al.
Xiuping Chen et al.
This study was conducted to determine the effect of microinjection of a single blastomere from in vitro fertilization (IVF)-derived eight-cell embryo into eight-cell cloned embryos harboring the gene encoding recombinant human lactoferrin (...
Enhanced reprogramming and cardiac differentiation of human keratinocytes derived from plucked hair follicles, using a single excisable lentivirus [0.03%]
利用单个可切除型慢病毒对拔取毛囊衍生的人角质细胞进行增强重编程和心肌分化
Atara Novak,Ronit Shtrichman,Igal Germanguz et al.
Atara Novak et al.
Induced pluripotent stem cells (iPSCs) represent an ideal cell source for future cell therapy and regenerative medicine. However, most iPSC lines described to date have been isolated from skin fibroblasts or other cell types that require ha...
Shuji Hirata,Hiroko Fukasawa,Sayaka Wakayama et al.
Shuji Hirata et al.
The successful generation of cloned animals and the establishment of embryonic stem (ES) cell lines from somatic cells suggest that these techniques may be used in human regenerative medicine. However, the fact that oocytes must be donated ...
Differences between cellular and molecular profiles of induced pluripotent stem cells generated from mouse embryonic fibroblasts [0.03%]
由小鼠胚胎成纤维细胞诱导产生的多能干细胞的细胞和分子谱系差异
Eun Young Kim,Kilsoo Jeon,Hyo Young Park et al.
Eun Young Kim et al.
Induced pluripotent stem (iPS) cells are a new alternative for the development of patient-specific stem cells, and the aim of this study was to determine whether differences exist between the cellular and molecular profiles of iPS cells, ge...