JungEun Park,HyunJu Oh,MinJung Kim et al.
JungEun Park et al.
Because cloned dogs are genetically identical, variations among these animals can be a useful tool to elucidate mechanisms underlying phenotypic differences. To estimate the influence of genetic factors on phenotypic variation, changes in c...
Viable calves produced by somatic cell nuclear transfer using meiotic-blocked oocytes [0.03%]
用减数分裂阻断卵母细胞核移植产生活牛犊
Tiago H C De Bem,Marcos R Chiaratti,Raquel Rochetti et al.
Tiago H C De Bem et al.
Somatic cell nuclear transfer (SCNT) has had an enormous impact on our understanding of biology and remains a unique tool for multiplying valuable laboratory and domestic animals. However, the complexity of the procedure and its poor effici...
3-Hydroxyflavone improves the in vitro development of cloned porcine embryos by inhibiting ROS production [0.03%]
3-羟基黄酮通过抑制ROS的产生改善克隆猪胚胎的体外发育
Sang Jun Uhm,Mukesh Kumar Gupta,Ziban Chandra Das et al.
Sang Jun Uhm et al.
UV-irradiation of oocytes during enucleation and serum starvation of donor cells during cell cycle synchronization may compromise the development competence of cloned embryos through excessive generation of reactive oxygen species (ROS). He...
The efficiency of cell fusion-based reprogramming is affected by the somatic cell type and the in vitro age of somatic cells [0.03%]
体细胞类型和体外培养时间影响基于细胞融合的重编程效率
Pollyanna Agnes Tat,Huseyin Sumer,Daniele Pralong et al.
Pollyanna Agnes Tat et al.
Cell fusion is one approach that has been used to demonstrate nuclear reprogramming of somatic cells to a pluripotent-like state and is a useful tool for screening factors involved in reprogramming. Recent cell fusion studies reported that ...
Mechanical phenotyping of mouse embryonic stem cells: increase in stiffness with differentiation [0.03%]
小鼠胚胎干细胞机械表型研究：分化过程中细胞刚性的增加
Anand Pillarisetti,Jaydev P Desai,Hamid Ladjal et al.
Anand Pillarisetti et al.
Atomic force microscopy (AFM) has emerged as a promising tool to characterize the mechanical properties of biological materials and cells. In our studies, undifferentiated and early differentiating mouse embryonic stem cells (mESCs) were as...
Ovine oocytes vitrified at germinal vesicle stage as cytoplast recipients for somatic cell nuclear transfer (SCNT) [0.03%]
玻璃化的卵母细胞作为细胞质受体进行体细胞核移植（SCNT）中的应用
Adel R Moawad,Inchul Choi,Jie Zhu et al.
Adel R Moawad et al.
The development of embryos produced by somatic cell nuclear transfer (SCNT) using vitrified oocytes as cytoplast recipients has been reported in cattle but not in sheep. This study investigated the parthenogenetic development of ovine oocyt...
Dynamic changes in epigenetic marks and gene expression during porcine epiblast specification [0.03%]
猪上胚层细胞分化过程中表观遗传标记和基因表达的动态变化
Yu Gao,Poul Hyttel,Vanessa Jane Hall
Yu Gao
Given the difficulties in establishing bona fide porcine embryonic stem cells, we considered it would be interesting to investigate histone modifications, X chromosome inactivation (XCI), deacetylation, DNA methylation, and gene expression ...
Utilization of human amniotic mesenchymal cells as feeder layers to sustain propagation of human embryonic stem cells in the undifferentiated state [0.03%]
人羊膜间充质细胞作为饲养层维持人类胚胎干细胞未分化状态的增殖能力研究
Kehua Zhang,Zhe Cai,Yang Li et al.
Kehua Zhang et al.
Human embryonic stem (ES) cells are usually maintained in the undifferentiated state by culturing on feeder cells layers of mouse embryonic fibroblasts (MEFs). However, MEFs are not suitable to support human ES cells used for clinical purpo...
Early senescence is not an inevitable fate of human-induced pluripotent stem-derived cells [0.03%]
人诱导多能干细胞分化细胞早衰并非不可避免
Maiko Gokoh,Miwako Nishio,Naoko Nakamura et al.
Maiko Gokoh et al.
Human-induced pluripotent stem cells (hiPSCs) are expected to become a powerful tool for regenerative medicine. Their efficacy in the use of clinical purposes is currently under intensive verification. It was reported that hiPSC-derived hem...
Trichostatin A modified histone covalent pattern and enhanced expression of pluripotent genes in interspecies black-footed cat cloned embryos but did not improve in vitro and in vivo viability [0.03%]
特拉霍斯汀A改变组蛋白共价模式并增强跨物种黑足猫克隆胚胎多能性基因表达但并未改善体外和体内存活率
Martha C Gómez,C Earle Pope,Monica N Biancardi et al.
Martha C Gómez et al.
Abstract The black-footed cat (BFC; Felis nigripes), one of the smallest wild cats, is listed as threatened. Interspecies somatic cell nuclear transfer (Is-SCNT) offers the possibility of preserving endangered species. Development to term o...