Follicular oocytes better support development in rabbit cloning than oviductal oocytes [0.03%]
卵泡卵细胞比输卵管卵细胞更好支持兔克隆胚胎的发育
Li-Ying Sung,Chien-Hong Chen,Jie Xu et al.
Li-Ying Sung et al.
This study was conducted to determine the effect of rabbit oocytes collected from ovaries or oviducts on the developmental potential of nuclear transplant embryos. Donor nuclei were obtained from adult skin fibroblasts, cumulus cells, and e...
Optimization of culture conditions to support long-term self-renewal of buffalo (Bubalus bubalis) embryonic stem cell-like cells [0.03%]
优化水牛（Bubalus bubalis）胚胎干细胞样细胞长期自我更新的文化条件
Ruchi Sharma,Aman George,Nitin Manchindra Kamble et al.
Ruchi Sharma et al.
A culture system capable of sustaining self-renewal of buffalo embryonic stem (ES) cell-like cells in an undifferentiated state over a long period of time was developed. Inner cell masses were seeded on KO-DMEM+15% KO-serum replacer on buff...
Global transcriptional analysis of oocyte-based and factor-based nuclear reprogramming in the nonhuman primate [0.03%]
非人灵长类动物卵母细胞和转录因子介导的体细胞核重编程的全局转录组分析
James Byrne
James Byrne
The mechanisms of nuclear reprogramming following somatic cell nuclear transfer (SCNT) to enucleated oocytes or factor-based reprogramming are poorly understood. In this study global transcriptional analysis was performed on a number of dif...
Shishu Huang,Victor Leung,Songlin Peng et al.
Shishu Huang et al.
Mesenchymal stem cells (MSCs) are a rare heterogeneous population of multipotent cells that can be isolated from many different adult and fetal tissues. They exhibit the capacity to give rise to cells of multiple lineages and are defined by...
Somatic cell-induced hyperacetylation, but not hypomethylation, positively and reversibly affects the efficiency of in vitro cloned blastocyst production in cattle [0.03%]
体细胞乙酰化诱导而非去甲基化正向和可逆地影响牛体细胞克隆囊胚的形成效率
Farnoosh Jafarpour,Sayed Morteza Hosseini,Mehdi Hajian et al.
Farnoosh Jafarpour et al.
5-Aza-2'-deoxycytidine (AzC), trichostatin A (TSA), and its natural mimetic, sodium butyrate (NaB), are antineoplastic drugs that can modify the epigenetic status of donor cells prior to somatic cell nuclear transfer (SCNT). In this study, ...
In vitro manipulation of mammalian preimplantation embryos can alter transcript abundance of histone variants and associated factors [0.03%]
体外操作可改变哺乳动物植入前胚胎组蛋白变体和相关因子的转录量
Georgia R Kafer,Peter L Kaye,Marie Pantaleon et al.
Georgia R Kafer et al.
Manipulation of mammalian embryos and gametes in vitro reduces viability. Specific causes for these reductions are still largely undetermined. Accumulating evidence suggests that survival rates and developmental competency may be reduced fo...
Scriptaid improves in vitro development and nuclear reprogramming of somatic cell nuclear transfer bovine embryos [0.03%]
scriptaid能改善体外培养的牛体细胞克隆胚胎的发育及重编程能力
Li-Jun Wang,Hui Zhang,Yong-Sheng Wang et al.
Li-Jun Wang et al.
The present study evaluated the effect of Scriptaid, a novel histone deacetylase inhibitor (HDACi), on the in vitro development of somatic cell nuclear transfer (SCNT) bovine embryos. Average fluorescence intensity of two epigenetic markers...
Cells, niche, fate: meeting report on the 6th International Meeting of the Stem Cell Network North Rhine Westphalia [0.03%]
细胞、生态位和命运：干细胞网络北威州第六次国际会议报告会
Stefan Radtke,Peter A Horn
Stefan Radtke
Reduced amino acids in the bovine uterine lumen of cloned versus in vitro fertilized pregnancies prior to implantation [0.03%]
体外克隆胚胎与受精胚胎在着床前子宫腔内氨基酸含量的差异分析
Anna E Groebner,Valeri Zakhartchenko,Stefan Bauersachs et al.
Anna E Groebner et al.
Fetal overgrowth and placental abnormalities frequently occur in pregnancies following somatic cell nuclear transfer (SCNT). An optimal intrauterine supply of amino acids (AA) is of specific importance for the development of the bovine prei...
Comparative Study
Cellular reprogramming. 2011 Oct;13(5):403-10. DOI:10.1089/cell.2011.0006 2011
Enucleation of demecolcine-treated bovine oocytes in cytochalasin-free medium: mechanism investigation and practical improvement [0.03%]
去壁细胞松弛素B溶液培养的牛卵母细胞成熟释放极体及胞质提取的研究与改进
Qinggang Meng,Xia Wu,Thomas D Bunch et al.
Qinggang Meng et al.
Demecolcine-assisted/induced enucleation has been used in nuclear transfer cloning procedures for many species, yet its mechanism of action remains unclear. Primarily because oocytoplasm protrusion induced by demecolcine is inhibited by the...