Slight improvement in full-term development of mouse somatic cell nuclear-transferred embryos by cotransfer of fertilized embryos [0.03%]
受精卵共移植改善体细胞克隆胚胎的发育效率
Yuta Tsuji,Yoko Kato,Yukio Tsunoda
Yuta Tsuji
In our previous study (Tsuji et al., 2010 ), administration of hCG to recipients around the timing of implantation significantly increased the in vivo development of mouse embryos after somatic cell nuclear transfer (SCNT) until day 10.5, b...
Comparative Study
Cellular reprogramming. 2012 Feb;14(1):38-44. DOI:10.1089/cell.2011.0047 2012
Development of intergeneric and intrageneric somatic cell nuclear transfer (SCNT) cat embryos and the determination of telomere length in cloned offspring [0.03%]
异属和同属的猫体细胞克隆胚胎的构建及其克隆子代的端粒长度分析
Sumeth Imsoonthornruksa,Anawat Sangmalee,Kanokwan Srirattana et al.
Sumeth Imsoonthornruksa et al.
Somatic cell nuclear transfer (SCNT) holds potential as a useful tool for agricultural and biomedical applications. In vitro development of marbled cat intergeneric SCNT reconstructed into domestic cat cytoplast revealed that cloned, marble...
Follicular growth-stimulated cows provide favorable oocytes for producing cloned embryos [0.03%]
促进卵泡生长的奶牛产生有利于克隆胚胎的卵母细胞
Satoshi Sugimura,Shuji Kobayashi,Yutaka Hashiyada et al.
Satoshi Sugimura et al.
We examined the influence of recipient oocytes on in vitro development, oxygen consumption, and gene expression in the resulting cloned bovine embryos. Oocytes derived from slaughterhouse ovaries and ovum pickup (OPU)-derived oocytes were u...
Comparative analysis of nuclear transfer embryo-derived mouse embryonic stem cells. Part II: gene regulation [0.03%]
核移植胚胎源小鼠胚胎干细胞的比较分析（第二部分）：基因调控
Julianna Kobolak,Marion Horsch,Sandra Geissler et al.
Julianna Kobolak et al.
In a mouse model nuclear transfer embryo-derived embryonic stem cell lines (ntESCs) of various genetic backgrounds and donor cell types were compared with reference ESCs and analyzed comprehensively at molecular level as a second part of a ...
Comparative Study
Cellular reprogramming. 2012 Feb;14(1):68-78. DOI:10.1089/cell.2011.0057 2012
Comparative analysis of nuclear transfer embryo-derived mouse embryonic stem cells. Part I: cellular characterization [0.03%]
核移植胚胎源小鼠胚胎干细胞的比较分析（第一部分）：细胞特性分析
Julianna Kobolak,Solomon Mamo,Ruttachuk Rungsiwiwut et al.
Julianna Kobolak et al.
Embryonic stem cells derived from nuclear transfer embryos (ntESCs) are particularly valuable for regenerative medicine, as they are a patient-specific and histocompatible cell source for the treatment of varying diseases. However, currentl...
Comparative Study
Cellular reprogramming. 2012 Feb;14(1):56-67. DOI:10.1089/cell.2011.0056 2012
Donor cells at the G1 phase enhance homogeneous gene expression among blastomeres in bovine somatic cell nuclear transfer embryos [0.03%]
供体细胞G1期可提高牛体细胞克隆囊胚均一表达比例
Daisaku Iwamoto,Aya Kasamatsu,Atsushi Ideta et al.
Daisaku Iwamoto et al.
The success rate of bovine somatic cell nuclear transfer (SCNT) embryos to full term has been reported to be higher with G1 cells than with G0 cells. To better understand the reason for this, we analyzed the kinetics of luminescence activit...
Cell colony formation induced by Xenopus egg extract as a marker for improvement of cloned blastocyst formation in the pig [0.03%]
非洲爪蟾卵提取物诱导的细胞集落形成作为改善猪克隆囊胚形成的标记
Ying Liu,Olga Østrup,Juan Li et al.
Ying Liu et al.
Treatment with cytoplasmic extracts from Xenopus laevis eggs represents a potential tool for universal cellular reprogramming. However, the biochemical activity and quality of the extract vary from batch to batch. This study aimed to evalua...
Efficient production and cellular characterization of sheep androgenetic embryos [0.03%]
高效生产及绵羊雄性胚胎的细胞学鉴定
Federica Zacchini,Marta Czernik,Domenico Iuso et al.
Federica Zacchini et al.
The production of androgenetic embryos in large animals is a complex procedure. Androgenetic embryos have been produced so far only in cattle and sheep using pronuclear transfer (PT) between zygotes derived from in vitro fertilization (IVF)...
Cobalt chloride pretreatment promotes cardiac differentiation of human embryonic stem cells under atmospheric oxygen level [0.03%]
氯化钴预处理促进人胚胎干细胞在常氧条件下向心肌细胞分化
Kwong-Man Ng,Yau-Chi Chan,Yee-Ki Lee et al.
Kwong-Man Ng et al.
Our previous study demonstrated the direct involvement of the HIF-1α subunit in the promotion of cardiac differentiation of murine embryonic stem cells (ESCs). We report the use of cobalt chloride to induce HIF-1α stabilization in human E...
Histone deacetylase inhibitor significantly improved the cloning efficiency of porcine somatic cell nuclear transfer embryos [0.03%]
组蛋白去乙酰化酶抑制剂可显著提高克隆猪胚胎的重编程效率
Yongye Huang,Xiaochun Tang,Wanhua Xie et al.
Yongye Huang et al.
Valproic acid (VPA), a histone deacetylase inbibitor, has been shown to generate inducible pluripotent stem (iPS) cells from mouse and human fibroblasts with a significant higher efficiency. Because successful cloning by somatic cell nuclea...