Dongmei Lai,Yifei Chen,Fangyuan Wang et al.
Dongmei Lai et al.
Human embryonic stem cells maintained on human amniotic epithelial cells (hESCs(hAEC)) are better preserved in an undifferentiated state and express pluripotency genes Oct4, Nanog, and Sox2 at higher levels compared with growth on mitotical...
Roscovitine treatment improves synchronization of donor cell cycle in G0/G1 stage and in vitro development of handmade cloned buffalo (Bubalus bubalis) embryos [0.03%]
罗库溴铵处理可改善手工克隆水牛（Bubalus bubalis）胚胎供体细胞周期的G0/G1同步化及体外发育效果
Naresh L Selokar,Monika Saini,Mushariffa Muzaffer et al.
Naresh L Selokar et al.
This study investigated the effects of serum-starvation, total confluence, and roscovitine treatment on cell-cycle synchronization of buffalo ear skin fibroblasts to the G0/G1 stage and on the developmental competence of cloned embryos. Ser...
Direct conversion of porcine embryonic fibroblasts into adipocytes by chemical molecules [0.03%]
化学小分子诱导猪胚胎成纤维细胞直接重编程为脂肪细胞
Jianguo Zhu,Daxin Pang,Yang Zhou et al.
Jianguo Zhu et al.
Direct reprogramming of terminally differentiated cells to specify different cell types may allow somatic cells to be reprogrammed to an alternative, differentiated fate without intervening stem or progenitor cells. Recent studies have show...
Valproic acid improves the in vitro development competence of bovine somatic cell nuclear transfer embryos [0.03%]
丙戊酸钠能改善牛体细胞克隆胚胎的体外发育潜能
Wenbing Xu,Yongsheng Wang,Yanyan Li et al.
Wenbing Xu et al.
The present study was carried out to examine the effect of valproic acid (VPA), an important histone deacetylase inhibitor, on the in vitro development and expression of the epigenetic marker histone H3 lysine 9 (H3K9ac) in bovine somatic c...
Reprogramming pig fetal fibroblasts reveals a functional LIF signaling pathway [0.03%]
猪胎儿成纤维细胞的重编程揭示了一个功能性的LIF信号通路
Alison J Thomson,Hadrien Pierart,Stephen Meek et al.
Alison J Thomson et al.
Distinct signaling pathways are reported to maintain pluripotency in embryo-derived stem cells. Mouse embryonic stem cells (ESCs) respond to leukemia inhibitory factor (LIF) and bone morphogenetic protein (BMP)-mediated activity, whereas hu...
Plasmid DNA size does affect nonviral gene delivery efficiency in stem cells [0.03%]
质粒DNA大小影响非病毒基因在干细胞中的递送效率
Sofia Ribeiro,Juergen Mairhofer,Catarina Madeira et al.
Sofia Ribeiro et al.
Genetic modification of stem cells, prior to transplantation, can enhance their survival and can improve their function in cell therapy settings. Mesenchymal stem cells (MSC) are considered one of the most promising tools for cell-based gen...
Human fetal liver stromal cells expressing erythropoietin promote hematopoietic development from human embryonic stem cells [0.03%]
人胚胎干细胞向造血细胞分化过程中体外添加EPO细胞促进造血发育
Chao Yang,Lei Ji,Wen Yue et al.
Chao Yang et al.
Blood cells transfusion and hematopoietic stem cells (HSCs) transplantation are important methods for cell therapy. They are widely used in the treatment of incurable hematological disorder, infectious diseases, genetic diseases, and immuno...
Gene expression profile normalization in cloned mice by trichostatin A treatment [0.03%]
曲古素酮处理克隆小鼠的基因表达规范化研究
Takashi Kohda,Satoshi Kishigami,Tomoko Kaneko-Ishino et al.
Takashi Kohda et al.
Cloning mammals by somatic cell nuclear transfer (SCNT) has become an established procedure, but the success rate remains low and gene expression abnormalities are also observed. In addition, SCNT pups exhibited an abnormal gene expression ...
Comparative Study
Cellular reprogramming. 2012 Feb;14(1):45-55. DOI:10.1089/cell.2011.0062 2012
Established preblastocyst- and blastocyst-derived ES cell lines have highly similar gene expression profiles, despite their differing requirements for derivation culture conditions [0.03%]
既定的囊前和囊胚期小鼠胚胎干细胞系的基因表达谱相似性较高，尽管它们对建系培养条件的需求不同
Chul Kim,Joonghoon Park,Tomokazu Amano et al.
Chul Kim et al.
The efficiency of embryonic stem (ES) cell derivation relies on an optimized culture medium and techniques for treating preimplantation stage embryos. Recently, ES cell derivation from the preblastocyst developmental stage was reported by r...
Comparative Study
Cellular reprogramming. 2012 Feb;14(1):1-7. DOI:10.1089/cell.2011.0048 2012
Chiara Sartori,Alex I DiDomenico,Alison J Thomson et al.
Chiara Sartori et al.
Pluripotential stem cells from livestock offer an exciting prospect for the biotechnology industry. Applying strategies established for the derivation of murine induced pluripotential stem cells (iPSCs), we have isolated ovine iPSCs that ca...