Forced expression of Nanog in human bone marrow-derived endothelial cells activates other six pluripotent genes [0.03%]
强制表达NANOG诱导人骨髓内皮细胞激活其他6个全能基因
Virginia Picanço-Castro,Elisa Russo-Carbolante,Dimas Tadeu Covas
Virginia Picanço-Castro
Human endothelial cells (ECs) have the ability to make up the lining of blood vessels. These cells are also capable of neovascularization and revascularization and have been applied in various clinical situations. With the aim of understand...
Equivalency of buffalo (Bubalus bubalis) embryonic stem cells derived from fertilized, parthenogenetic, and hand-made cloned embryos [0.03%]
由受精卵、孤雌激活卵和体细胞克隆胚胎建立的水牛胚胎干细胞系具有发育全能性且等效性相当
Musharifa Muzaffar,Naresh L Selokar,Karn P Singh et al.
Musharifa Muzaffar et al.
This study was aimed at establishing buffalo embryonic stem cells (ESCs) from in vitro fertilized (IVF), parthenogenetic, and hand-made cloned (HMC) embryos and to check their equivalency in terms of stem cell marker expression, longevity, ...
Comparative Study
Cellular reprogramming. 2012 Jun;14(3):267-79. DOI:10.1089/cell.2011.0090 2012
Temporal repression of endogenous pluripotency genes during reprogramming of porcine induced pluripotent stem cells [0.03%]
猪诱导多能干细胞重新编程过程中内源多能基因的时序抑制作用研究
Vanessa J Hall,Marianne Kristensen,Mikkel A Rasmussen et al.
Vanessa J Hall et al.
Porcine induced pluripotent stem cells (piPSCs) have the capacity to differentiate in vitro and in vivo and form chimeras. However, the lack of transgene silencing of exogenous DNA integrated into the genome and the inability of cells to pr...
Full-term development of gaur-bovine interspecies somatic cell nuclear transfer embryos: effect of trichostatin A treatment [0.03%]
曲古茋素A处理对杂交渐进式体细胞核移植胚胎全程发育的影响
Kanokwan Srirattana,Sumeth Imsoonthornruksa,Chuti Laowtammathron et al.
Kanokwan Srirattana et al.
Trichostatin A (TSA) has previously been used in somatic cell nuclear transfer (SCNT) to improve the cloning efficiency in several species, which led our team to investigate the effects of TSA on the full-term development of bovine SCNT and...
Clinical Trial
Cellular reprogramming. 2012 Jun;14(3):248-57. DOI:10.1089/cell.2011.0099 2012
Transplanted bone marrow cells do not provide new oocytes but rescue fertility in female mice following treatment with chemotherapeutic agents [0.03%]
骨髓移植细胞不能提供新的卵泡但能救治化疗后的小鼠的生育能力
Nicolas Santiquet,Luc Vallières,François Pothier et al.
Nicolas Santiquet et al.
It is generally accepted that mammalian females are born with a finite pool of oocytes and that this is the sole source of ovules throughout the reproductive life of the adult. This dogma was shaken in 2003 when researchers showed that the ...
Treatment of nuclear-donor cells or cloned zygotes with chromatin-modifying agents increases histone acetylation but does not improve full-term development of cloned cattle [0.03%]
细胞核供体或克隆卵裂球处理去乙酰化酶抑制剂可提高组蛋白乙酰化水平但不能改善牛克隆胚胎的发育性能
Juliano Rodrigues Sangalli,Tiago Henrique Camara De Bem,Felipe Perecin et al.
Juliano Rodrigues Sangalli et al.
Although somatic cell nuclear transfer (SCNT) is a promising tool, its potential use is hampered by the high mortality rates during the development to term of cloned offspring. Abnormal epigenetic reprogramming of donor nuclei after SCNT is...
Clinical Trial
Cellular reprogramming. 2012 Jun;14(3):235-47. DOI:10.1089/cell.2011.0079 2012
Early aberrations in chromatin dynamics in embryos produced under in vitro conditions [0.03%]
体外受精胚胎早期染色质动态异常
Rahul S Deshmukh,Olga Østrup,Frantisek Strejcek et al.
Rahul S Deshmukh et al.
In vitro production of porcine embryos by means of in vitro fertilization (IVF) or somatic cell nuclear transfer (SCNT) is limited by great inefficienciy. The present study investigated chromatin and nucleolar dynamics in porcine embryos de...
Parallel differentiation of embryonic stem cells into different cell types by a single gene-based differentiation system [0.03%]
基于单一基因的分化系统平行诱导胚胎干细胞向多种细胞类型分化
Eva C Thoma,Katja Maurus,Toni U Wagner et al.
Eva C Thoma et al.
The generation of defined somatic cell types from pluripotent stem cells represents a promising system for many applications for regenerative therapy or developmental studies. Certain key developmental genes have been shown to be able to in...
Enucleated ovine oocyte supports human somatic cells reprogramming back to the embryonic stage [0.03%]
去核绵羊卵母细胞支持人体细胞编程回复到胚胎阶段
S Morteza Hosseini,Mehdi Hajian,Mohsen Forouzanfar et al.
S Morteza Hosseini et al.
Increased possibility of universality of ooplasmic reprogramming factors resulted in a parallel increased interest to use interspecies somatic cell nuclear transfer (iSCNT) to address basic questions of developmental biology and to improve ...
Feeder-free and serum-free production of hepatocytes, cholangiocytes, and their proliferating progenitors from human pluripotent stem cells: application to liver-specific functional and cytotoxic assays [0.03%]
不含 feeder 层和血清的人类多能干细胞向肝细胞、胆管细胞及其增殖前体分化的 feeder-free 和无血清系统：在肝脏特异性功能和细胞毒性分析中的应用
Naoko Nakamura,Kumiko Saeki,Masami Mitsumoto et al.
Naoko Nakamura et al.
We have established a serum- and feeder-free culture system for the efficient differentiation of multifunctional hepatocytes from human embryonic stem (ES) cells and three entirely different induced pluripotent stem (iPS) cells (including v...