The mechanism of oocyte activation influences the cell cycle-related genes expression during bovine preimplantation development [0.03%]
卵子活化机制影响奶牛围植入前胚胎细胞周期相关基因的表达
Marcella Pecora Milazzotto,Weber Beringui Feitosa,Fabiola Feitosa Paula-Lopes et al.
Marcella Pecora Milazzotto et al.
The first cleavage divisions and preimplantation embryonic development are supported by mRNA and proteins synthesized and stored during oogenesis. Thus, mRNA molecules of maternal origin decrease and embryonic development becomes gradually ...
Expression of innate immune response genes in liver and three types of adipose tissue in cloned pigs [0.03%]
克隆猪肝脏及三种脂肪组织中固有免疫反应基因的表达
Tina Rødgaard,Kerstin Skovgaard,Jan Stagsted et al.
Tina Rødgaard et al.
The pig has been proposed as a relevant model for human obesity-induced inflammation, and cloning may improve the applicability of this model. We tested the assumptions that cloning would reduce interindividual variation in gene expression ...
Effects of combined treatment of MG132 and scriptaid on early and term development of porcine somatic cell nuclear transfer embryos [0.03%]
MG132和Scriptaid联合处理对体细胞克隆猪胚胎早期及胚期发育的影响
Jiude Mao,Kimberly Tessanne,Kristin M Whitworth et al.
Jiude Mao et al.
Although improving, the efficiency of producing offspring by somatic cell nuclear transfer (SCNT) is still low (
Generation of neuronal progenitor cells and neurons from mouse sleeping beauty transposon-generated induced pluripotent stem cells [0.03%]
由睡美人转座子生成的小鼠诱导多能干细胞向神经前体细胞和神经元的分化
Nuttha Klincumhom,Melinda K Pirity,Sara Berzsenyi et al.
Nuttha Klincumhom et al.
Mouse embryonic stem cells (ESCs) and induced pluripotent stem (iPS) cells can be used as models of neuronal differentiation for the investigation of mammalian neurogenesis, pharmacological testing, and development of cell-based therapies. ...
Chemically assisted enucleation results in higher G6PD expression in early bovine female embryos obtained by somatic cell nuclear transfer [0.03%]
化学去核能提高体细胞克隆胚胎早期雌性原肠前胚胎的G6PD表达水平
Naiara Zoccal Saraiva,Clara Slade Oliveira,Tatiane Almeida Drummond Tetzner et al.
Naiara Zoccal Saraiva et al.
Despite extensive efforts, low efficiency is still an issue in bovine somatic cell nuclear transfer (SCNT). The hypothesis of our study was that the use of cytoplasts produced by chemically assisted enucleation (EN) would improve nuclear re...
Stemness of human Wharton's jelly mesenchymal cells is maintained by floating cultivation [0.03%]
人Wharton胶间充质干细胞的干性在悬浮培养中得以保持
Osamu Higuchi,Motonori Okabe,Toshiko Yoshida et al.
Osamu Higuchi et al.
Abstract Recently, the search for stem cells has become focused on fetal appendages such as the amniotic membrane and umbilical cord. Previously, we have shown the existence of stem cells in the amniotic membrane that can differentiate into...
Effects of activation on functional aster formation, microtubule assembly, and blastocyst development of goat oocytes injected with round spermatids [0.03%]
激活对山羊圆精子细胞注射卵母细胞功能性中心体形成、微管装配及 blastocyst 发育的影响
Xin-Yong Liu,Yi-Long Miao,Jie Zhang et al.
Xin-Yong Liu et al.
A systematic study was conducted on round spermatids (ROS) injection (ROSI) using the goat model. After ROSI, the oocytes were treated or not with ionomycin (ROSI+I and ROSI-I, respectively) and compared with intracytoplasmic sperm injectio...
Improved bovine ICSI outcomes by sperm selected after combined heparin-glutathione treatment [0.03%]
硫酸肝素-谷胱甘肽处理后的精子用于卵胞浆内注射可提高受精率及胚胎发育率
Mohammad Hadi Sekhavati,Farshad Shadanloo,Morteza S Hosseini et al.
Mohammad Hadi Sekhavati et al.
Despite widespread application of intracytoplasmic sperm injection (ICSI) in human-assisted reproductive techniques (ART), the efficiency of this method is still far from satisfactory in livestock, particularly in the bovine species with it...
Human placenta-derived mesenchymal stem cells with silk fibroin biomaterial in the repair of articular cartilage defects [0.03%]
人胎盘间充质干细胞结合丝素蛋白生物材料修复关节软骨缺损
Fang Li,Yong-Zhen Chen,Zong-Ning Miao et al.
Fang Li et al.
Cartilage tissue engineering requires a porous biodegradable scaffold and nonimmunogenic cells with chondrogenic potential. In this study, the ability of the placenta-derived mesenchymal stem cells (PMSCs) to grow on silk fibroin (SF) bioma...
Improved cloning efficiency and developmental potential in bovine somatic cell nuclear transfer with the oosight imaging system [0.03%]
新型卵母细胞选择系统提高体细胞克隆胚胎的发育率
Eun Young Kim,Min Jee Park,Hyo Young Park et al.
Eun Young Kim et al.
In somatic cell nuclear transfer (SCNT) procedures, exquisite enucleation of the recipient oocyte is critical to cloning efficiency. The purpose of this study was to compare the effects of two enucleation systems, Hoechst staining and UV ir...
Comparative Study
Cellular reprogramming. 2012 Aug;14(4):305-11. DOI:10.1089/cell.2011.0103 2012