Effects of donor fibroblast cell type and transferred cloned embryo number on the efficiency of pig cloning [0.03%]
供体成纤维细胞类型及移植克隆胚胎数量对猪克隆效率的影响
Zicong Li,Junsong Shi,Dewu Liu et al.
Zicong Li et al.
Currently, cloning efficiency in pigs is very low. Donor cell type and number of cloned embryos transferred to an individual surrogate are two major factors that affect the successful rate of somatic cell nuclear transfer (SCNT) in pigs. Th...
Expression pattern of pluripotent markers in different embryonic developmental stages of buffalo (Bubalus bubalis) embryos and putative embryonic stem cells generated by parthenogenetic activation [0.03%]
水牛胚胎不同发育阶段多能性标记物表达模式及孤雌激活诱导的干细胞系的建立与鉴定
Karn P Singh,Ramakant Kaushik,Veena Garg et al.
Karn P Singh et al.
In this study, we describe the production of buffalo parthenogenetic blastocysts and subsequent isolation of parthenogenetic embryonic stem cell (PGESC)-like cells. PGESC colonies exhibited dome-shaped morphology and were clearly distinguis...
Establishment of bovine embryonic stem cell lines using a minimized feeder cell drop [0.03%]
利用最小化饲养细胞滴培养建立牛胚胎干细胞系
Eun Young Kim,Eun Ji Noh,Hyo Young Park et al.
Eun Young Kim et al.
Bovine embryonic stem cells (ESCs) are a powerful tool for agricultural and biomedical applications. The purpose of this study was to introduce a new method for generating bovine ESCs. Mechanically isolated bovine inner cell masses (ICMs) f...
Telomere elongation during morula-to-blastocyst transition in cloned porcine embryos [0.03%]
克隆猪胚胎有丝分裂期至囊胚期的端粒延长能力受损
Thanh Quang Dang-Nguyen,Seiki Haraguchi,Satoshi Akagi et al.
Thanh Quang Dang-Nguyen et al.
Although telomeres are elongated during morula-to-blastocyst transition in cloned embryos, it is still unknown whether donor cell types have any effect on this elongation. In the present study, we examined the changes of telomere length dur...
Intermediate filaments promote nuclear mechanical constraints during somatic cell nuclear transfer in the mouse [0.03%]
中间丝在小鼠体细胞核移植过程中的作用
Laurence Gall,Vincent Brochard,Sylvie Ruffini et al.
Laurence Gall et al.
The somatic cell nuclear transfer (SCNT) procedure requires nuclear remodeling to return differentiated somatic nuclei to the totipotent undifferentiated stage. We hypothesize that mechanical constraints might occur upon SCNT and thereby af...
Induced pluripotent stem cells generated from adult bone marrow-derived cells of the nonhuman primate (Callithrix jacchus) using a novel quad-cistronic and excisable lentiviral vector [0.03%]
采用新型四顺反子和可消除型慢病毒载体从非人灵长类动物（绒毛猴）成体骨髓来源细胞中诱导生成多能干细胞
Anastasia Wiedemann,Kathrin Hemmer,Inga Bernemann et al.
Anastasia Wiedemann et al.
Regenerative medicine is in need of solid, large animal models as a link between rodents and humans to evaluate the functionality, immunogenicity, and clinical safety of stem cell-derived cell types. The common marmoset (Callithrix jacchus)...
Induction of pluripotent stem cells from a cynomolgus monkey using a polycistronic simian immunodeficiency virus-based vector, differentiation toward functional cardiomyocytes, and generation of stably expressing reporter lines [0.03%]
基于多顺反子的猴免疫缺陷病毒载体诱导食蟹猴多能干细胞、向功能型心肌细胞分化及稳定表达报告基因系的建立
Stephanie Wunderlich,Alexandra Haase,Sylvia Merkert et al.
Stephanie Wunderlich et al.
Induced pluripotent stem cells (iPSCs) represent a novel cell source for regenerative therapies. Many emerging iPSC-based therapeutic concepts will require preclinical evaluation in suitable large animal models. Among the large animal speci...
Induced pluripotent stem cells: progress and future perspectives in the stem cell world [0.03%]
诱导多能干细胞：于干细胞世界中的进展与未来前景
Habib Rezanejad,Maryam M Matin
Habib Rezanejad
Pluripotent stem cells (PSCs) have the potential to differentiate into many cell types and therefore can be a valuable source for cell therapy. Embryonic stem cells (ESCs), which are derived from the inner cell mass (ICM) of the blastocyst,...
Generation of porcine-induced pluripotent stem cells by using OCT4 and KLF4 porcine factors [0.03%]
利用OCT4和KLF4猪诱导因子生成猪多能干细胞
Kai Liu,Guangzhen Ji,Jian Mao et al.
Kai Liu et al.
Induced pluripotent stem cells (iPSCs) can be artificially reprogrammed from somatic cells by overexpression of exogenous transcription factors. The pig has increasingly become an important large animal model for preclinical tests and studi...
Oxamflatin improves developmental competence of porcine somatic cell nuclear transfer embryos [0.03%]
奥沙美坦改善猪体细胞克隆胚胎的发育潜能
Sol-Ji Park,Hee-Jung Park,Ok-Jae Koo et al.
Sol-Ji Park et al.
Abstract Aberrant epigenetic nuclear reprogramming of somatic nuclei is a major cause of low success in cloning. It has been demonstrated that treatment of histone deacetylase inhibitors (HDACi) enhances developmental potential of somatic c...