α-1,3-Galactosyltransferase knockout pig induced pluripotent stem cells: a cell source for the production of xenotransplant pigs [0.03%]
α-1,3-半乳糖基转移酶敲除猪诱导多能干细胞：异种移植猪的细胞来源
Yubing Liu,Jeong Yeh Yang,Yangqing Lu et al.
Yubing Liu et al.
The shortage of human organs and tissues for transplant has led to significant interest in xenotransplantation of pig tissues for human patients. However, transplantation of pig organs results in an acute immune rejection, leading to death ...
Higher methylation in genomic DNA indicates incomplete reprogramming in induced pluripotent stem cells [0.03%]
基因组DNA的高甲基化表明诱导多能干细胞再编程不完全
Wenping Zhou,Kun Wang,Weimin Ruan et al.
Wenping Zhou et al.
Pluripotent stem cells can be created successfully through the inner cell mass (ICM), nuclear transfer, and defined-factor induction. Unfortunately, the epigenetic characteristics of the cells produced are poorly understood. In this article...
Adenovector-mediated gene delivery to human umbilical cord mesenchymal stromal cells induces inner ear cell phenotype [0.03%]
人源脐带间充质干细胞的腺病毒介导基因传递诱导耳蜗细胞表型
Keerthana Devarajan,M Laird Forrest,Michael S Detamore et al.
Keerthana Devarajan et al.
Hearing is one of our main sensory systems and having a hearing disorder can have a significant impact in an individual's quality of life. Sensory neural hearing loss (SNHL) is the most common form of hearing loss; it results from the degen...
Clinical Trial
Cellular reprogramming. 2013 Feb;15(1):43-54. DOI:10.1089/cell.2011.0097 2013
Cloned sheep blastocysts derived from oocytes enucleated manually using a pulled pasteur pipette [0.03%]
手动使用巴斯德吸管抽核卵母细胞克隆羊的胚囊
S M Hosseini,M Hajian,F Moulavi et al.
S M Hosseini et al.
The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes ...
Development of an embryoid body-based screening strategy for assessing the hepatocyte differentiation potential of human embryonic stem cells following single-cell dissociation [0.03%]
基于胚胎体的筛选策略的发展，用于评估人胚胎干细胞在单细胞解离后向肝细胞分化的潜能
Sebastian Greenhough,Helen Bradburn,John Gardner et al.
Sebastian Greenhough et al.
We have devised an embryoid body-based screening method for the selection of human embryonic stem cell (hESC) lines capable of forming functional hepatocyte-like cells (HLCs) after single-cell dissociation. The screening method highlighted ...
The choice of expression vector promoter is an important factor in the reprogramming of porcine fibroblasts into induced pluripotent cells [0.03%]
猪成纤维细胞诱导建系过程中供体基因表达载体启动子的选择是一个重要因素
Stoyan Petkov,Poul Hyttel,Heiner Niemann
Stoyan Petkov
Porcine induced pluripotent stem cells (iPSCs) are an important animal model for development of regenerative therapies in human medicine. To date, the majority of the porcine cell lines with iPSC characteristics have been generated with the...
Selective isolation of nanog-positive human amniotic mesenchymal cells and differentiation into cardiomyocytes [0.03%]
人羊膜基质中分离NANOG阳性细胞并诱导分化为心肌细胞
Shingo Otaka,Saori Nagura,Chika Koike et al.
Shingo Otaka et al.
Adult cardiomyocytes have little ability to regenerate, thus cardiac regeneration therapy represents a potential method for treating severe heart failure. Human amniotic mesenchymal cells (hAMCs) have the potential to be a useful cell sourc...
Establishment and characterization of immortalized human amniotic epithelial cells [0.03%]
人绒毛膜促性腺激素永生化的人羊膜上皮细胞的建立和特征描述
Kaixuan Zhou,Chika Koike,Toshiko Yoshida et al.
Kaixuan Zhou et al.
Human amniotic epithelial cells (HAEs) have a low immunogenic profile and possess potent immunosuppressive properties. HAEs also have several characteristics similar to stem cells, and they are discarded after parturition. Thus, they could ...
Effects of donor fibroblasts expressing OCT4 on bovine embryos generated by somatic cell nuclear transfer [0.03%]
表达OCT4的供体成纤维细胞对体细胞核移植犏牛胚胎的影响
Marcelo D Goissis,Steven T Suhr,Jose B Cibelli
Marcelo D Goissis
The production of healthy, live, cloned animals by somatic cell nuclear transfer (SCNT) has been hampered by low efficiencies. Significant epigenetic changes must take place to ensure proper chromatin remodeling in SCNT. We hypothesized tha...
Hypoxia supports reprogramming of mesenchymal stromal cells via induction of embryonic stem cell-specific microRNA-302 cluster and pluripotency-associated genes [0.03%]
缺氧通过诱导胚胎干细胞特异性微小核糖核酸-302簇和多能性相关基因支持间充质基质细胞重编程
Sabine Foja,Matthias Jung,Bernadette Harwardt et al.
Sabine Foja et al.
Pluripotency is characterized by specific transcription factors such as OCT4, NANOG, and SOX2, but also by pluripotency-associated microRNAs (miRs). Somatic cells can be reprogrammed by forced expression of these factors leading to induced ...