Histone deacetylase inhibitor improves the development and acetylation levels of cat-cow interspecies cloned embryos [0.03%]
组蛋白去乙酰化酶抑制剂改善猫山羊interspecies克隆胚胎的发育及乙酰化水平
Manita Wittayarat,Yoko Sato,Lanh Thi Kim Do et al.
Manita Wittayarat et al.
Abnormal epigenetic reprogramming, such as histone acetylation, might cause low efficiency of interspecies somatic cell nuclear transfer (iSCNT). This study was conducted to evaluate the effects of trichostatin A (TSA) on the developmental ...
Zebularine and scriptaid significantly improve epigenetic reprogramming of yak fibroblasts and cloning efficiency [0.03%]
新型表观遗传重编程化合物显著提升牦牛体细胞核移植效率
Xianrong Xiong,Daoliang Lan,Jian Li et al.
Xianrong Xiong et al.
Abnormal epigenetic reprogramming of the donor nucleus after somatic cell nuclear transfer (SCNT) is thought to be the main cause of low cloning efficiency. Following SCNT, the donor nucleus often fails to express early embryonic genes and ...
SCF/KIT inhibition has a cumulative but reversible effect on the self-renewal of embryonic stem cells and on the survival of differentiating cells [0.03%]
SCF/KIT抑制对胚胎干细胞的自我更新和分化细胞的存活具有累积但可逆的影响
Lindsay Fraser,A Helen Taylor,Lesley M Forrester
Lindsay Fraser
The receptor tyrosine kinase c-KIT is expressed in embryonic stem cells (ESCs) and adult stem cells, and many functional studies have demonstrated the importance of the SCF/KIT signaling pathway in adult stem cell maintenance. In this study...
Proteomic analysis of early reprogramming events in murine somatic cells incubated with Xenopus laevis oocyte extracts demonstrates network associations with induced pluripotency markers [0.03%]
蛋白质组分析小鼠体细胞与非洲爪蟾卵母细胞抽提物共孵育早期重新编程事件表明与诱导多能性标志物的网络关联
Alex J Rathbone,Susan Liddell,Keith H S Campbell
Alex J Rathbone
The reprogramming of somatic cells into a pluripotent/embryonic-like state holds great potential for regenerative medicine, bypassing ethical issues associated with embryonic stem cells (ESCs). Numerous methods, including somatic cell nucle...
Specific activation requirements of zona-free sheep oocytes before and after somatic cell nuclear transfer [0.03%]
体细胞核移植前后去透明带绵羊卵母细胞的激活要求
M Hajian,M Kiani,M S Hosseini et al.
M Hajian et al.
In this study, the effect of the steps involved in zona-free somatic cell nuclear transfer (SCNT) on oocyte transcripts was investigated in sheep. To establish the reliable combined electrical-chemical activation for zona-free oocytes, oocy...
Comparative Study
Cellular reprogramming. 2013 Jun;15(3):247-57. DOI:10.1089/cell.2012.0089 2013
Effects of epidermal growth factor and basic fibroblast growth factor on the proliferation and osteogenic and neural differentiation of adipose-derived stem cells [0.03%]
表皮生长因子和碱性成纤维细胞生长因子对脂肪来源干细胞增殖及向骨系和神经系分化影响的实验研究
Feihu Hu,Xiu Wang,Gaofeng Liang et al.
Feihu Hu et al.
Stem cells used for clinical tissue regeneration therapy should have the capacity of self-renewal, high proliferation, and differentiation and be able to be transplanted in large numbers. Although high concentrations of epidermal growth fac...
Human bone marrow mesenchymal stem cells support the derivation and propagation of human induced pluripotent stem cells in culture [0.03%]
人骨髓间充质干细胞支持诱导多能干细胞的体外衍生和增殖
Lifei Zhang,Weiyan Zheng,Yebo Wang et al.
Lifei Zhang et al.
Human induced pluripotent stem cells (hiPSCs) need to be generated and expanded under clinically applicable culture conditions before they can be used for clinical application. In this study, we demonstrate that inactivated human mesenchyma...
Comparative Study
Cellular reprogramming. 2013 Jun;15(3):216-23. DOI:10.1089/cell.2012.0064 2013
Stromal cell-derived factor-1 receptor CXCR4-overexpressing bone marrow mesenchymal stem cells accelerate wound healing by migrating into skin injury areas [0.03%]
CXCR4过表达的骨髓间充质干细胞通过迁移到皮肤损伤区域来加速伤口愈合
Dazhi Yang,Shijin Sun,Zhengguo Wang et al.
Dazhi Yang et al.
Stromal cell-derived factor-1 (SDF-1) and its membrane receptor C-X-C chemokine receptor type 4 (CXCR4) are involved in the homing and migration of multiple stem cell types, neovascularization, and cell proliferation. This study investigate...
Comparative Study
Cellular reprogramming. 2013 Jun;15(3):206-15. DOI:10.1089/cell.2012.0046 2013
Germ-line-competent embryonic stem cells of the Chinese Kunming mouse strain with long-term self-renewal ability [0.03%]
具有长期自我更新能力的昆明小鼠囊胚期地胚胎干细胞系及其生殖系传递
Xinrong Peng,Tao Liu,Baotian Yang et al.
Xinrong Peng et al.
Kunming (KM) mice are the most widely used strain in China. However, authentic embryonic stem cells (ESCs) from KM mice have never been available, and this hampers the genetic manipulation of this valuable mice strain. In this study, we sho...
Cloning changes the response to obesity of innate immune factors in blood, liver, and adipose tissues in domestic pigs [0.03%]
克隆改变家猪血液、肝脏和脂肪组织中固有免疫因子对肥胖的反应
Tina Rødgaard,Kerstin Skovgaard,Jan Stagsted et al.
Tina Rødgaard et al.
The objective of this study was to evaluate the usefulness of cloned pigs as porcine obesity models reflecting obesity-associated changes in innate immune factor gene expression profiles. Liver and adipose tissue expression of 43 innate imm...
Comparative Study
Cellular reprogramming. 2013 Jun;15(3):185-94. DOI:10.1089/cell.2012.0091 2013