Imprinted genes and satellite loci are differentially methylated in bovine somatic cell nuclear transfer clones [0.03%]
牛体细胞核转移克隆中印记基因和卫星序列的甲基化状态不同
Chih-Jie Shen,Chiao-Chieh Lin,Perng-Chih Shen et al.
Chih-Jie Shen et al.
In mammals, genome-wide epigenetic reprogramming systems exist in primordial germ cells and zygotes. These reprogramming systems play crucial roles in regulating genome functions during critical stages of embryonic development, and they con...
Alternating current electric fields of varying frequencies: effects on proliferation and differentiation of porcine neural progenitor cells [0.03%]
不同频率的交流电场对猪神经干细胞增殖和分化的影响
Ji-Hey Lim,Seth D McCullen,Jorge A Piedrahita et al.
Ji-Hey Lim et al.
Application of sinusoidal electric fields (EFs) has been observed to affect cellular processes, including alignment, proliferation, and differentiation. In the present study, we applied low-frequency alternating current (AC) EFs to porcine ...
He was my mentor [0.03%]
他曾经是我的导师
Teruhiko Wakayama
Teruhiko Wakayama
DNA aneuploidy in porcine bone marrow-derived mesenchymal stem cells undergoing osteogenic and adipogenic in vitro differentiation [0.03%]
体外向骨细胞和脂肪细胞分化过程中猪骨髓间充质干细胞的DNA倍体异常变化
Jolanta Opiela,Marcin Samiec,Michał Bochenek et al.
Jolanta Opiela et al.
In this study, we estimated the distribution of DNA diploidy and aneuploidy in porcine mesenchymal stem cells (pMSCs) that were subjected to osteoblast/osteocyte and adipocyte differentiation to determine the impact of long-term in vitro cu...
Alena Langerova,Helena Fulka,Josef Fulka Jr
Alena Langerova
Embryonic stem cells are mostly derived from mature oocytes that were either fertilized or activated parthenogenetically and then reached the blastocyst stage. From the cell cycle perspective, fertilization or activation induces the exit fr...
Prof. Keith Campbell: a personal perspective of a friend and colleague [0.03%]
凯斯·坎贝尔教授：一位朋友和同事的个人视角
William Ritchie
William Ritchie
Chromatin-modifying agents reactivate embryonic renal stem/progenitor genes in human adult kidney epithelial cells but abrogate dedifferentiation and stemness [0.03%]
染色质修饰剂可活化人类成人肾上皮细胞中的胚胎肾干细胞/前体基因，但会消除去分化和干性特征
Dorit Omer,Orit Harari-Steinberg,Ella Buzhor et al.
Dorit Omer et al.
Recent studies have suggested that epigenetic modulation with chromatin-modifying agents can induce stemness and dedifferentiation and increase developmental plasticity. For instance, valproic acid (VPA), a histone deacetylase (HDAC) inhibi...
Transgenic chicken, mice, cattle, and pig embryos by somatic cell nuclear transfer into pig oocytes [0.03%]
转入猪卵母细胞的转基因鸡、小鼠、牛和猪体细胞核移植胚胎
Mukesh Kumar Gupta,Ziban Chandra Das,Young Tae Heo et al.
Mukesh Kumar Gupta et al.
This study explored the possibility of producing transgenic cloned embryos by interspecies somatic cell nuclear transfer (iSCNT) of cattle, mice, and chicken donor cells into enucleated pig oocytes. Enhanced green florescent protein (EGFP)-...
Gene expression of Dnmt1 isoforms in porcine oocytes, embryos, and somatic cells [0.03%]
猪卵母细胞、胚胎和体细胞中Dnmt1异构体的基因表达
Angelica M Giraldo,Kristi DeCourcy,Suyapa F Ball et al.
Angelica M Giraldo et al.
In the mouse, the dynamics of genomic methylation and the initial events of gametic imprinting are controlled by the activity of an oocyte isoform of the DNA methyltransferase-1 (Dnmt1o) enzyme. The objectives of this study were to identify...
An improved protocol for isolation and culturing of mouse spermatogonial stem cells [0.03%]
小鼠 spermatogonial 干细胞分离培养的改进方法
Maryam Baazm,Farid Abolhassani,Mahdi Abbasi et al.
Maryam Baazm et al.
Spermatogonial stem cells (SSCs) represent a unique testicular cell type that has the capacity for proliferating, differentiating, and transmitting genetic information. This particular cell type is a strong focus of stem cell research, with...