Methylated DNA immunoprecipitation and high-throughput sequencing (MeDIP-seq) using low amounts of genomic DNA [0.03%]
基于高通量测序的甲基化DNA免疫沉淀技术(MeDIP-seq)及其在微量基因组DNA样品中的应用研究
Ming-Tao Zhao,Jeffrey J Whyte,Garrett M Hopkins et al.
Ming-Tao Zhao et al.
DNA modifications, such as methylation and hydroxymethylation, are pivotal players in modulating gene expression, genomic imprinting, X-chromosome inactivation, and silencing repetitive sequences during embryonic development. Aberrant DNA m...
Cellular extract facilitates nuclear reprogramming by altering DNA methylation and pluripotency gene expression [0.03%]
细胞提取物通过改变DNA甲基化和多能性基因表达来促进核重编程
Xian-Rong Xiong,Dao-Liang Lan,Jian Li et al.
Xian-Rong Xiong et al.
The functional reprogramming of a differentiated cell to a pluripotent state presents potential beneficial applications in disease mechanisms and regenerative medicine. Epigenetic modifications enable differentiated cells to perpetuate mole...
Positive correlation between the efficiency of induced pluripotent stem cells and the development rate of nuclear transfer embryos when the same porcine embryonic fibroblast lines are used as donor cells [0.03%]
相同细胞系作为供体核移植重组胚发育与诱导多能干细胞生成效率的正相关性研究
Bingteng Xie,Jianyu Wang,Shichao Liu et al.
Bingteng Xie et al.
Induced pluripotent stem cells (iPSCs) and nuclear transfer (NT) are two of the primary routes to reprogram differentiated cells back to the pluripotent state. However, it is still unknown whether there is any correlation between the reprog...
Therapeutic potential of adipose stem cells in tissue repair of irradiated skeletal muscle in a rabbit model [0.03%]
脂肪干细胞在兔放射性骨骼肌损伤修复中治疗潜力的研究
Xinchu Ni,Wei Sun,Suping Sun et al.
Xinchu Ni et al.
The repairing function and differentiation potency of adipose stem cells (ASCs) transplantation following skeletal muscle injury induced by radiotherapy are still not well defined. In this study, one side of the buttocks of 64 New Zealand w...
Kiho Lee,Chunmin Wang,Lee Spate et al.
Kiho Lee et al.
The possibility of fertilization without male contribution to the embryonic genome was investigated in pig oocytes. Mature oocytes were co-incubated with sperm, and in an attempt to prevent the incorporation of the sperm head into the oopla...
Ultrastructural characteristics of three undifferentiated mouse embryonic stem cell lines and their differentiated three-dimensional derivatives: a comparative study [0.03%]
三种小鼠胚胎干细胞系及其分化三维衍生细胞的超微结构特征比较研究
Suzan Alharbi,Mona Elsafadi,Mohammed Mobarak et al.
Suzan Alharbi et al.
The fine structures of mouse embryonic stem cells (mESCs) grown as colonies and differentiated in three-dimensional (3D) culture as embryoid bodies (EBs) were analyzed by transmission electron microscopy. Undifferentiated mESCs expressed ma...
Comparative Study
Cellular reprogramming. 2014 Apr;16(2):151-65. DOI:10.1089/cell.2013.0073 2014
Virginia Picanço-Castro,Lilian F Moreira,Simone Kashima et al.
Virginia Picanço-Castro et al.
Pluripotent stem cells, both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), have the ability to differentiate into several cell types that can be used in drug testing and also in the study and treatment of diseases....
Sodium butyrate facilitates reprogramming by derepressing OCT4 transactivity at the promoter of embryonic stem cell-specific miR-302/367 cluster [0.03%]
丁酸钠通过去抑制OCT4在胚胎干细胞特异性miR-302/367聚簇启动子的转录活性来促进重新编程
Zhonghui Zhang,Di Xiang,Wen-Shu Wu
Zhonghui Zhang
Small-molecule inhibitors and microRNAs (miRNAs) are two newly emerging classes of tools for optimizing induced pluripotent stem cell (iPSC) generation. We report here that sodium butyrate (NaB), a small-molecule inhibitor of histone deacet...
Posttranslational modifications of defined embryonic reprogramming transcription factors [0.03%]
定义的胚胎重新编程转录因子的翻译后修饰
Suresh Ramakrishna,Kwang-Soo Kim,Kwang-Hyun Baek
Suresh Ramakrishna
The generation of induced pluripotent stem cells (iPSCs) from somatic cells by expressing ectopic reprogramming transcriptional factors such as Oct3/4, Sox2, Klf4, c-Myc, and Nanog is one of the cutting-edge discoveries in stem cell and can...
Improving viability and transfection efficiency with human umbilical cord wharton's jelly cells through use of a ROCK inhibitor [0.03%]
通过使用ROCK抑制剂改善人脐带沃顿凝胶细胞的活力和转染效率
Adam J Mellott,Megan E Godsey,Heather E Shinogle et al.
Adam J Mellott et al.
Differentiating stem cells using gene delivery is a key strategy in tissue engineering and regenerative medicine applications. Nonviral gene delivery bypasses several safety concerns associated with viral gene delivery; however, leading non...
Clinical Trial
Cellular reprogramming. 2014 Apr;16(2):91-7. DOI:10.1089/cell.2013.0069 2014