Vitamin C supplementation enhances compact morulae formation but reduces the hatching blastocyst rate of bovine somatic cell nuclear transfer embryos [0.03%]
维生素C补充促进牛体细胞克隆胚胎发育至致密 morula 阶段，但降低孵化囊胚率
Qian Li,Yong-Sheng Wang,Li-Jun Wang et al.
Qian Li et al.
Vitamin C, an antioxidant that reduces reactive oxygen species (ROS) in cells, is capable of significantly improving the developmental competence of porcine and mouse somatic cell nuclear transfer (SCNT) embryos, both in vitro and in vivo. ...
Effects of histone deacetylase inhibitor oxamflatin on in vitro porcine somatic cell nuclear transfer embryos [0.03%]
组蛋白脱乙酰基酶抑制剂oxamflatin对体外猪核移植胚胎的影响
Liming Hou,Fanhua Ma,Jinzeng Yang et al.
Liming Hou et al.
Low cloning efficiency is considered to be caused by the incomplete or aberrant epigenetic reprogramming of differentiated donor cells in somatic cell nuclear transfer (SCNT) embryos. Oxamflatin, a novel class of histone deacetylase inhibit...
Three-day-old human unfertilized oocytes after in vitro fertilization/intracytoplasmic sperm injection can be activated by calcium ionophore a23187 or strontium chloride and develop to blastocysts [0.03%]
体外受精/卵胞浆内单精子注射后三天的未受精人类卵子可用钙离子载体a23187或氯化锶激活并发育成囊胚期胚胎
Ying Liu,Xiao-Jie Han,Ming-Hui Liu et al.
Ying Liu et al.
Our objective was to observe the effectiveness of the calcium ionophore A23187 or strontium chloride on the activation and subsequent embryonic development of 3-day-old human unfertilized oocytes after in vitro fertilization (IVF) or intrac...
The small molecule inhibitors PD0325091 and CHIR99021 reduce expression of pluripotency-related genes in putative porcine induced pluripotent stem cells [0.03%]
小分子抑制剂PD0325901和CHIR99021能降低假定的猪诱导多能干细胞中与多能性相关的基因的表达
Stoyan Petkov,Poul Hyttel,Heiner Niemann
Stoyan Petkov
Small molecule inhibitors of the mitogen-activated protein kinase kinase (MEK) and glycogen synthesis kinase 3 (Gsk3) have been essential in the establishment and maintenance of embryonic stem cells (ESCs) from rats and from nonpermissive m...
Histone deacetylase inhibitor induces the expression of select epithelial genes in mouse utricle sensory epithelia-derived progenitor cells [0.03%]
组蛋白去乙酰化酶抑制剂诱导小鼠utricle感觉上皮源性干细胞中选择性上皮基因的表达
Zhengqing Hu,Jue Wang
Zhengqing Hu
Mouse utricle sensory epithelial cell-derived progenitor cells (MUCs), which have hair cell progenitor and mesenchymal features via epithelial-to-mesenchymal transition (EMT) as previously described, provide a potential approach for hair ce...
Recombinant human growth differentiation factor-9 improves oocyte reprogramming competence and subsequent development of bovine cloned embryos [0.03%]
重组人源生长分化因子-9能提高卵母细胞重编程能力及克隆胚胎的发育潜力
Jianmin Su,Guangdong Hu,Yongsheng Wang et al.
Jianmin Su et al.
Previously, we found that oocyte-secreted factors (OSFs) secreted by denuded oocytes during in vitro maturation (IVM) enhance subsequent development of bovine somatic cell nuclear transfer (SCNT) embryos. This treatment requires many oocyte...
Gene expression profiles of similarly derived human embryonic stem cell lines correlate with their distinct propensity to exit stemness and their different differentiation behavior in culture [0.03%]
具有不同分化行为的人胚胎干细胞系退出干性的基因表达谱特征分析
Oliver Sterthaus,Anne-Catherine Feutz,Hong Zhang et al.
Oliver Sterthaus et al.
Four normal-karyotype human embryonic stem cell (hESC) lines were generated using the same protocol and maintained under identical conditions. Despite these precautions, gene expression patterns were found to be dissimilar among the four li...
Hongyan Sun,Guomin Zhang,Fulu Dong et al.
Hongyan Sun et al.
Induced pluripotent stem cells (iPSCs) have potential applications in the restoration of fertility, regenerative medicine, and animal biotechnology. In this study, we present the induction of iPSCs from mouse Sertoli cells (SCs) by introduc...
Huseyin Sumer,Kitai Kim,Jun Liu et al.
Huseyin Sumer et al.
Embryonic stem cells (ESCs) have previously been reported to reprogram somatic cells following fusion. The resulting ES-somatic cell hybrids have been shown to adopt the transcriptional profile of ESCs, suggesting that the pluripotent progr...
Improvement of cloned embryos development by co-culturing with parthenotes: a possible role of exosomes/microvesicles for embryos paracrine communication [0.03%]
孤雌激活卵子旁植对改善克隆胚胎发育及可能通过外泌体/微囊泡介导的胚胎旁分泌通讯机制的作用
Islam M Saadeldin,Su Jin Kim,Yoo Bin Choi et al.
Islam M Saadeldin et al.
It is well known that embryos cultured in a group can create a microenvironment through secretion of autocrine and paracrine factors that can support and improve the embryos' development when compared to the embryos cultured individually. I...