Electrophysiological profiles of induced neurons converted directly from adult human fibroblasts indicate incomplete neuronal conversion [0.03%]
直接由成人人体成纤维细胞诱导产生的神经元的电生理学特征表明其不完全具备神经元功能
Peter Koppensteiner,Stefan Boehm,Ottavio Arancio
Peter Koppensteiner
The direct conversion of human fibroblasts to neuronal cells, termed human induced neuronal (hiN) cells, has great potential for future clinical advances. However, previous studies have not provided an in-depth analysis of electrophysiologi...
Development and gene expression of porcine cloned embryos derived from bone marrow stem cells with overexpressing Oct4 and Sox2 [0.03%]
携带过表达Oct4和Sox2的骨髓干细胞衍生猪克隆胚胎的发育及基因表达研究
Jeong-Hyeon Lee,Won-Jae Lee,Ryoung-Hoon Jeon et al.
Jeong-Hyeon Lee et al.
The present study compared the potential of porcine bone marrow mesenchymal stem cells (pBMSCs) at different passages as nuclear transfer (NT) donors and the developmental efficiency of NT embryos from donor cells transfected with/without O...
MicroRNA-34c expression in donor cells influences the early development of somatic cell nuclear transfer bovine embryos [0.03%]
供体细胞中微小核糖核酸-34c的表达影响体细胞克隆牛早期胚胎发育
Bo Wang,Yongsheng Wang,Man Zhang et al.
Bo Wang et al.
The essence of the reprogramming activity of somatic cell nuclear transfer (SCNT) embryos is to produce normal fertilized embryos. However, reprogramming of somatic cells is not as efficient as the reprogramming of sperm. In this report, we...
Effects of histone acetylation status on the early development of in vitro porcine transgenic cloned embryos [0.03%]
组蛋白乙酰化状态对体外转基因克隆猪胚胎早期发育的影响
Biping Luo,Shiqiang Ju,Caroline W Muneri et al.
Biping Luo et al.
The purpose of this study was to investigate the effects of the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) on transgene expression and development of porcine transgenic cloned embryos, specifically focusing on effects derived...
Yifei Chen,Dongmei Lai
Yifei Chen
Since human embryonic stem cells (hESCs) were first isolated and successfully cultured in vitro, the pluripotent potential of hESCs has been underestimated. The pluripotency of mouse embryonic stem cells (mESCs) can be categorized as naïve...
Impaired neural differentiation potency by retinoic acid receptor-α pathway defect in induced pluripotent stem cells [0.03%]
视黄酸受体α通路缺陷通过损害神经分化潜能影响诱导多能干细胞的功能
Pei-Shan Hou,Wen-Chin Huang,Wei Chiang et al.
Pei-Shan Hou et al.
Induced pluripotent stem cells (iPSCs) are reprogrammed from somatic cells via ectopic gene expression and, similarly to embryonic stem cells (ESCs), possess powerful abilities to self-renew and differentiate into cells of various lineages....
Generation of human β-thalassemia induced pluripotent cell lines by reprogramming of bone marrow-derived mesenchymal stromal cells using modified mRNA [0.03%]
用mRNA对骨髓间质细胞进行重编程以建立人类β-地中海贫血的多能干细胞系
Ioanna Varela,Angeliki Karagiannidou,Vasilis Oikonomakis et al.
Ioanna Varela et al.
Synthetic modified mRNA molecules encoding pluripotency transcription factors have been used successfully in reprogramming human fibroblasts to induced pluripotent stem cells (iPSCs). We have applied this method on bone marrow-derived mesen...
Henrik Callesen,Ying Liu,Hanne S Pedersen et al.
Henrik Callesen et al.
The low efficiency in obtaining piglets after production of cloned embryos was challenged in two steps-first by performing in vitro culture for 5-6 days after cloning to obtain later-stage embryos for more precise selection for transfer, an...
Identification of animals produced by somatic cell nuclear transfer using DNA methylation in the retrotransposon-like 1 promoter [0.03%]
利用反转座子样1启动子中的DNA甲基化鉴别克隆动物
Christine Couldrey,Paul Maclean,David N Wells
Christine Couldrey
Public perception of somatic cell nuclear transfer (SCNT) in the production of agricultural animals is surrounded by fear, which is exacerbated by the inability to differentiate animals generated by SCNT from those generated by natural mati...
Trisomy 21 mid-trimester amniotic fluid induced pluripotent stem cells maintain genetic signatures during reprogramming: implications for disease modeling and cryobanking [0.03%]
唐氏综合症中期羊水诱导多能干细胞在重新编程过程中保持基因特征：对疾病建模和冷冻库的启示
Caterina Pipino,Sayandip Mukherjee,Anna L David et al.
Caterina Pipino et al.
Trisomy 21 is the most common chromosomal abnormality and is associated primarily with cardiovascular, hematological, and neurological complications. A robust patient-derived cellular model is necessary to investigate the pathophysiology of...