Downregulation of DNA methyltransferase 1 in zona-free cloned buffalo (Bubalus bubalis) embryos by small interefering RNA improves in vitro development but does not alter DNA methylation level [0.03%]
用siRNA下调游离卵周隙水牛克隆胚胎DNA甲基转移酶1基因的表达对其体外发育的影响及对DNA甲基化水平无影响
Naresh L Selokar,Monika Saini,Himanshu Agrawal et al.
Naresh L Selokar et al.
Aberrant epigenetic reprogramming, especially genomic hypermethylation, is implicated as the primary reason behind the failure of the cloning process during somatic cell nuclear transfer (SCNT). We transfected one-cell-stage zona-free buffa...
In vitro induction of human adipose-derived stem cells into lymphatic endothelial-like cells [0.03%]
体外诱导人脂肪来源干细胞向淋巴内皮样细胞分化
Yi Yang,Xiao-hu Chen,Fu-gui Li et al.
Yi Yang et al.
Human adipose-derived stem cells (hADSCs) may provide a suitable number of progenitors for the treatment of lymphatic edema; however, to date the protocols for inducing hADSCs into this tissue type have not been standardized. We wished to i...
Expression and function of the ID1 gene during transforming growth factor-β1-induced differentiation of human embryonic stem cells to endothelial cells [0.03%]
ID1基因在转化生长因子-β1诱导的人胚胎干细胞向内皮细胞分化过程中的表达及其功能研究
Qing Huan,Yan Wang,Lixia Yang et al.
Qing Huan et al.
ID1 can mediate transforming growth factor-β (TGF-β)/activin receptor-like kinase-1 (ALK1)-induced (and Smad-dependent) migration in endothelial cells (ECs). However, the role that ID1 plays during differentiation of human embryonic stem ...
A highly optimized protocol for reprogramming cancer cells to pluripotency using nonviral plasmid vectors [0.03%]
一种使用非病毒质粒载体将癌细胞重编为多能干细胞的高度优化的方案
Hongzhi Zhao,Timothy J Davies,Jiaolin Ning et al.
Hongzhi Zhao et al.
In spite of considerable interest in the field, reprogramming induced pluripotent stem cells (iPSCs) directly from cancer cells has encountered considerable challenges, including the extremely low reprogramming efficiency and instability of...
Assessment of difference in gene expression profile between embryos of different derivations [0.03%]
不同来源胚胎基因表达差异的分析
Sujin Kwon,Sangkyun Jeong,Young Sun Jeong et al.
Sujin Kwon et al.
Researchers have exerted sustained efforts to improve the viability of somatic cell nuclear transfer (SCNT) embryos, testing their experimental designs and probing the resultant embryos. However, the lack of a reliable method to estimate th...
Comparative Study
Cellular reprogramming. 2015 Feb;17(1):49-58. DOI:10.1089/cell.2014.0057 2015
Oxamflatin treatment enhances cloned porcine embryo development and nuclear reprogramming [0.03%]
奥沙酚酯处理可促进克隆猪胚胎发育和核重编程
Jiude Mao,Ming-Tao Zhao,Kristin M Whitworth et al.
Jiude Mao et al.
Faulty epigenetic reprogramming of somatic nuclei is thought to be the main reason for low cloning efficiency by somatic cell nuclear transfer (SCNT). Histone deacetylase inhibitors (HDACi), such as Scriptaid, improve developmental competen...
The effects of ROCK inhibitor Y-27632 on injectable spheroids of bovine corneal endothelial cells [0.03%]
ROCK抑制剂Y-27632对牛角膜内皮细胞可注射类球体的影响
Yonglong Guo,Qing Liu,Yan Yang et al.
Yonglong Guo et al.
The spheroids of 3-dimensional culture and Rho-associated kinase (ROCK) inhibitor Y-27632 have shown many advantages for the promotion of cellular viability and proliferation. The objective of this study was to investigate the effect of Y-2...
Ovine induced pluripotent stem cells are resistant to reprogramming after nuclear transfer [0.03%]
卵形多能干细胞核移植后仍具有诱导重编程的特性
Sergio D German,Keith H S Campbell,Elisabeth Thornton et al.
Sergio D German et al.
Induced pluripotent stem cells (iPSCs) share similar characteristics of indefinite in vitro growth with embryonic stem cells (ESCs) and may therefore serve as a useful tool for the targeted genetic modification of farm animals via nuclear t...
β-Cell differentiation of human pancreatic duct-derived cells after in vitro expansion [0.03%]
体外培养的人胰管来源细胞分化为β细胞
Elisa Corritore,Erica Dugnani,Valentina Pasquale et al.
Elisa Corritore et al.
β-Cell replacement therapy is a promising field of research that is currently evaluating new sources of cells for clinical use. Pancreatic epithelial cells are potent candidates for β-cell engineering, but their large-scale expansion has ...