Production of a Cloned Buffalo (Bubalus bubalis) Calf from Somatic Cells Isolated from Urine [0.03%]
从尿液中分离的体细胞生产克隆水牛(Bubalus bubalis)幼崽
Pankaj K Madheshiya,Amol A Sahare,Basanti Jyotsana et al.
Pankaj K Madheshiya et al.
This study was aimed at isolation of cells from urine and skin on the ventral part of the tails of healthy adult female buffaloes (Bubalus bubalis), an area rarely exposed to solar radiation, establishment of the cells in culture, and their...
Zhiliang Zhao,Mengyao Xu,Meng Wu et al.
Zhiliang Zhao et al.
Cellular differentiation is usually considered to be an irreversible process during development due to robust lineage commitment. Feedback and feed-forward loops play a significant role in maintaining lineage-specific gene expression proces...
Effects of Scriptaid on the Histone Acetylation, DNA Methylation and Development of Buffalo Somatic Cell Nuclear Transfer Embryos [0.03%]
Scriptaid对水牛体细胞克隆胚胎组蛋白乙酰化、DNA甲基化和发育的影响
Hongliang Sun,Fenghua Lu,Peng Zhu et al.
Hongliang Sun et al.
The present study was undertaken to examine the effect of Scriptaid treatment on histone acetylation, DNA methylation, expression of genes related to histone acetylation, and development of buffalo somatic cell nuclear transfer (SCNT) embry...
Effective Oocyte Vitrification and Survival Techniques for Bovine Somatic Cell Nuclear Transfer [0.03%]
有效的牛体细胞核移植卵母细胞玻璃化和存活技术
Min Jee Park,Seung Eun Lee,Eun Young Kim et al.
Min Jee Park et al.
Bovine somatic cell nuclear transfer (SCNT) using vitrified-thawed (VT) oocytes has been studied; however, the cloning efficiency of these oocytes is not comparable with that of nonvitrified (non-V) fresh oocytes. This study sought to optim...
Buffalo embryos produced by handmade cloning from oocytes selected using brilliant cresyl blue staining have better developmental competence and quality and are closer to embryos produced by in vitro fertilization in terms of their epigenetic status and gene expression pattern [0.03%]
采用优良结晶紫染色筛选卵母细胞手工克隆的水牛胚胎发育潜能和质量更好，其表观遗传状态和基因表达模式更接近于体外受精胚胎
Sushil K Mohapatra,Anjit Sandhu,Venkata S Neerukattu et al.
Sushil K Mohapatra et al.
We compared handmade cloned (HMC) buffalo blastocysts produced from oocytes stained with Brilliant Cresyl Blue (BCB) and classified into those with blue (BCB+) or colorless cytoplasm (BCB-). The blastocyst rate was higher (p
Derivation and characterization of bovine induced pluripotent stem cells by transposon-mediated reprogramming [0.03%]
利用转座子重新编程获得并表征牛诱导性多能干细胞
Thirumala R Talluri,Dharmendra Kumar,Silke Glage et al.
Thirumala R Talluri et al.
Induced pluripotent stem cells (iPSCs) are a seminal breakthrough in stem cell research and are promising tools for advanced regenerative therapies in humans and reproductive biotechnology in farm animals. iPSCs are particularly valuable in...
Timing factors affecting blastocyst development in equine somatic cell nuclear transfer [0.03%]
影响体细胞核移植胚胎囊胚发育的时序因素
Young-Ho Choi,Isabel C Velez,Beatriz Macías-García et al.
Young-Ho Choi et al.
In nuclear transfer (NT), exposure of donor cell chromatin to the ooplast cytoplasm may aid reprogramming; however, the length of exposure feasible is limited by the developmental life span of the oocyte. We examined the effect of duration ...
Deficiency of genomic reprogramming in trophoblast stem cells following nuclear transfer [0.03%]
核移植后滋养干细胞的基因组重编程缺陷
Hidehiko Ogawa,Hiroyuki Watanabe,Atsushi Fukuda et al.
Hidehiko Ogawa et al.
To examine the genomic reprogrammability of trophoblast stem (TS) cells using a nuclear transfer technique, we produced TS cloned embryos using five TS cell lines from three strains of mice (ICR, B6D2F1, and B6CBF1) as donors and observed d...
Early development of cloned bovine embryos produced from oocytes enucleated by fluorescence metaphase II imaging using a conventional halogen-lamp microscope [0.03%]
采用传统卤素灯显微镜荧光中期II成像法去卵母细胞克隆牛早期胚胎的发育情况
Daisaku Iwamoto,Kazuo Yamagata,Masao Kishi et al.
Daisaku Iwamoto et al.
Enucleation of a recipient oocyte is one of the key processes in the procedure of somatic cell nuclear transfer (SCNT). However, especially in bovine species, lipid droplets spreading in the ooplasm hamper identification and enucleation of ...
Prolonged proteasome inhibition cyclically upregulates Oct3/4 and Nanog gene expression, but reduces induced pluripotent stem cell colony formation [0.03%]
蛋白酶体长期抑制可周期性上调OCT3/4和NANOG基因表达，但会减少诱导多能干细胞集落形成数量
Z Elizabeth Floyd,Jaroslaw Staszkiewicz,Rachel A Power et al.
Z Elizabeth Floyd et al.
There is ample evidence that the ubiquitin-proteasome system is an important regulator of transcription and its activity is necessary for maintaining pluripotency and promoting cellular reprogramming. Moreover, proteasome activity contribut...