Generation of Human Induced Pluripotent Stem Cells from Extraembryonic Tissues of Fetuses Affected by Monogenic Diseases [0.03%]
从单基因疾病胎儿的额外胚胎组织中生成人类诱导多能干细胞
Paola Spitalieri,Rosa V Talarico,Annalisa Botta et al.
Paola Spitalieri et al.
The generation of human induced pluripotent stem cells (hiPSCs) derived from an autologous extraembryonic fetal source is an innovative personalized regenerative technology that can transform own-self cells into embryonic stem-like ones. Th...
Identification of Valid Housekeeping Genes for Real-Time Quantitative PCR Analysis of Collapsed Lung Tissues of Neonatal Somatic Cell Nuclear Transfer-Derived Cattle [0.03%]
新生体细胞核移植黄牛肺泡塌陷组织实时荧光定量PCR实验中管家基因的鉴定
Yan Liu,Yunhai Zhang,Qiuling Jiang et al.
Yan Liu et al.
Cloned calves produced by somatic cell nuclear transfer frequently suffer alveolar collapse as newborns. To study the underlying pathophysiological mechanisms responsible for this phenomenon, the expression profiles of numerous genes involv...
Transcriptome Profiling Reveals Degree of Variability in Induced Pluripotent Stem Cell Lines: Impact for Human Disease Modeling [0.03%]
转录组分析揭示诱导多能干细胞系的差异性：对人类疾病建模的影响
Jens Schuster,Jonatan Halvardson,Laureanne Pilar Lorenzo et al.
Jens Schuster et al.
Induced pluripotent stem cell (iPSC) technology has become an important tool for disease modeling. Insufficient data on the variability among iPSC lines derived from a single somatic parental cell line have in practice led to generation and...
Thymic Epithelial Cells Induced from Pluripotent Stem Cells by a Three-Dimensional Spheroid Culture System Regenerates Functional T Cells in Nude Mice [0.03%]
三维类球体培养体系诱导多功能干细胞成为胸腺上皮细胞并支持裸鼠功能性T淋巴细胞的重建
Motohito Okabe,Sachiko Ito,Naomi Nishio et al.
Motohito Okabe et al.
The thymus is mainly composed of two types of epithelial cells, medullary thymic epithelial cells and cortex thymic epithelial cells (mTECs and cTECs). The tissue structure and mechanism for T cell development are complicated, with generati...
Goo Jang,ByeongChun Lee
Goo Jang
As man's best friend, dogs have an important position in human society. Ten years ago, we reported the first cloned dog, and his birth has raised various scientific issues, such as those related to health, reproduction, and life span. He ha...
Handmade Cloned Buffalo (Bubalus bubalis) Embryos Produced from Somatic Cells Isolated from Milk and Ear Skin Differ in Their Developmental Competence, Epigenetic Status, and Gene Expression [0.03%]
由牛奶和耳皮肤分离的体细胞制作的水 buffalo 人工克隆胚胎发育潜能、表观遗传状态及基因表达不同
Basanti Jyotsana,Amol A Sahare,Anuj K Raja et al.
Basanti Jyotsana et al.
We compared the cloning efficiency of buffalo embryos produced by handmade cloning (HMC) using ear skin- and milk-derived donor cells. The blastocyst rate was lower (p < 0.05) for milk-derived than that for skin-derived embryos, whereas the...
Characterization and Multilineage Differentiation of Domestic and Black-Footed Cat Mesenchymal Stromal/Stem Cells from Abdominal and Subcutaneous Adipose Tissue [0.03%]
家猫和黑足猫腹部和皮下脂肪组织间充质基质/干细胞的特征及多向分化能力研究
Martha C Gómez,Qian Qin,Monica N Biancardi et al.
Martha C Gómez et al.
Transplantation of mesenchymal stem cells (MSCs) isolated from bone marrow or adipose tissue is emerging as a promising tool for cell replacement therapy and regenerative medicine in domestic and endangered animal species. Defining the diff...
OP9 Stromal Cells Proteins Involved in Hematoendothelial Differentiation from Human Embryonic Stem Cells [0.03%]
人胚胎干细胞向血内皮分化的血管基质细胞相关蛋白研究
Lilian M Figueiredo,Everton B O Costa,Maristela D Orellana et al.
Lilian M Figueiredo et al.
Hematopoietic cells (HCs) and endothelial cells (ECs) can be produced in vitro from human embryonic stem cells (hESCs), but the differentiation systems used are still inefficient. To overcome this obstacle, it is necessary to understand the...
Inducible Lentivirus-Mediated Expression of the Oct4 Gene Affects Multilineage Differentiation of Adult Human Bone Marrow-Derived Mesenchymal Stem Cells [0.03%]
四环素诱导型 lentiviral 构建体表达Oct4基因影响人骨髓间充质干细胞向多系分化
Qiang Hao,Jia-Qiang An,Fei Hao et al.
Qiang Hao et al.
The octamer-binding transcription factor 4 (Oct4) gene plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) and reprogramming adult somatic cells into induced pluripotent stem cells (iPSCs). In th...
Development, Characterization, and Pluripotency Analysis of Buffalo (Bubalus bubalis) Embryonic Stem Cell Lines Derived from In Vitro-Fertilized, Hand-Guided Cloned, and Parthenogenetic Embryos [0.03%]
由体外受精、人工引导克隆和孤雌激活胚胎建立的水 buffalo胚胎干细胞系的建系、鉴定及多能性分析
Syed Mohmad Shah,Neha Saini,Syma Ashraf et al.
Syed Mohmad Shah et al.
We present the derivation, characterization, and pluripotency analysis of three buffalo embryonic stem cell (buESC) lines, from in vitro-fertilized, somatic cell nuclear-transferred, and parthenogenetic blastocysts. These cell lines were de...