Effects of Activin in Embryoid Bodies Expressing Fibroblast Growth Factor 5 [0.03%]
活化素在表达成纤维细胞生长因子5的类胚胎体中的作用
Yasumasa Shirouzu,Goichi Yanai,Kai-Chiang Yang et al.
Yasumasa Shirouzu et al.
Nodal/activin signaling is indispensable for embryonic development. We examined what activin does to the embryoid bodies (EBs) produced from mouse embryonic stem cells (mESCs) expressing an epiblast marker. The EBs were produced by culturin...
Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity [0.03%]
通过阻断芳烃受体内源性活性提高鸡原始生殖细胞的体内维持
Juan M Pérez Sáez,Leonardo E Bussmann,J Lino Barañao et al.
Juan M Pérez Sáez et al.
Primordial germ cells (PGCs) are the undifferentiated progenitors of gametes. Germline competent PGCs can be developed as a cell-based system for genetic modification in chickens, which provides a valuable tool for transgenic technology wit...
Phenotypes of Aging Postovulatory Oocytes After Somatic Cell Nuclear Transfer in Mice [0.03%]
基于小鼠体细胞核移植的过成熟卵母细胞老化表型研究
Ah Reum Lee,Takashi Shimoike,Teruhiko Wakayama et al.
Ah Reum Lee et al.
Oocytes rapidly lose their developmental potential after ovulation, termed postovulatory oocyte aging, and often exhibit characteristic phenotypes, such as cytofragmentation, abnormal spindle shapes, and chromosome misalignments. Here, we r...
Exposure of Somatic Cells to Cytoplasm Extracts of Porcine Oocytes Induces Stem Cell-Like Colony Formation and Alters Expression of Pluripotency and Chromatin-Modifying Genes [0.03%]
体细胞与猪卵母细胞细胞质提取物接触诱导干细胞样集落的形成并改变多能性和染色质修饰基因的表达
Werner Giehl Glanzner,Eliza R Komninou,Ashwini Mahendran et al.
Werner Giehl Glanzner et al.
Cell permeabilization followed by exposure to cytoplasmic extracts of oocytes has been proposed as an alternative to transduction of transcription factors for inducing pluripotency in cultured somatic cells. The main goal in this study was ...
Rex Rabbit Somatic Cell Nuclear Transfer with In Vitro-Matured Oocytes [0.03%]
体外成熟的卵母细胞核移植重构胚的发育能力及食蟹猴桑葚胚抗凝集蛋白在克隆猴中的作用研究
Yong Liu,Huili Wang,Jinhua Lu et al.
Yong Liu et al.
Somatic cell nuclear transfer (SCNT) requires large numbers of matured oocytes. In vitro-matured (IVM) oocytes have been used in SCNT in many animals. We investigated the use of IVM oocytes in Rex rabbit SCNT using Rex rabbit ovaries obtain...
Effects of Insulin-like Growth Factor-1 on Development of Somatic Cell Cloned Bovine Embryos [0.03%]
胰岛素样生长因子1对牛体细胞克隆胚胎发育的影响研究
Pengxiang Qu,Yanyan Li,Tengfei Deng et al.
Pengxiang Qu et al.
The aim of this study was to assess the effect of insulin-like growth factor-1 (IGF-1) on the developmental competence of somatic cell nuclear transfer (SCNT) bovine embryos. First, the expression levels of IGF-1 receptor (IGF-1R) and IGF-1...
Production of Bovine Embryos and Calves Cloned by Nuclear Transfer Using Mesenchymal Stem Cells from Amniotic Fluid and Adipose Tissue [0.03%]
来源于羊水和脂肪组织间充质干细胞的体细胞核移植及牛胚胎和克隆牛的生产
Carolina Gonzales da Silva,Carlos Frederico Martins,Tereza Cristina Cardoso et al.
Carolina Gonzales da Silva et al.
The less differentiated the donor cells are used in nuclear transfer (NT), the more easily are they reprogrammed by the recipient cytoplasm. In this context, mesenchymal stem cells (MSCs) appear as an alternative to donor nuclei for NT. The...
Improving the Efficacy of Cryopreservation of Spermatogonia Stem Cells by Antioxidant Supplements [0.03%]
抗氧化剂补充改善 spermatogonia 干细胞 cryopreservation 的效果
Fereshte Aliakbari,Mohamad Ali Sedighi Gilani,Fardin Amidi et al.
Fereshte Aliakbari et al.
Cryopreservation of spermatogonial stem cells (SSCs) is an applicable method for young males seeking fertility preservation before starting a treatment. It increases reactive oxygen species (ROS) formation and oxidative stress, which damage...
Muse Cells, a New Type of Pluripotent Stem Cell Derived from Human Fibroblasts [0.03%]
由人类纤维母细胞诱导而来的新型多能干细胞——缪斯细胞
Qi Liu,Ru-zhi Zhang,Di Li et al.
Qi Liu et al.
A new type of mesenchymal stem cells (MSCs) that expresses stage-specific embryonic antigen 3 (SSEA-3) and the mesenchymal cell marker CD105 are known as multilineage-differentiating stress-enduring (Muse) cells. Studies have shown that ste...
Rat Blastocysts from Nuclear Injection and Time-Lagged Enucleation and Their Commitment to Embryonic Stem Cells [0.03%]
核移植与胞核抽取延时后的鼠 blastocyst 及其向胚胎干细胞分化的能力
Hiromasa Hara,Teppei Goto,Akiko Takizawa et al.
Hiromasa Hara et al.
Pronucleus-like vesicle formation following premature chromosome condensation (PCC) of the donor cell nucleus is the key event for successful generation of cloned rodents by nuclear transplantation (NT). However in rat cloning, this change ...