Production of Pigs by Hand-Made Cloning Using Mesenchymal Stem Cells and Fibroblasts [0.03%]
利用间充质干细胞和成纤维细胞进行手工克隆生产猪
Zhenzhen Yang,Gábor Vajta,Ying Xu et al.
Zhenzhen Yang et al.
Mesenchymal stem cells (MSCs) exhibited self-renewal and less differentiation, making the MSCs promising candidates for adult somatic cell nuclear transfer (SCNT). In this article, we tried to produce genome identical pigs through hand-made...
MEF2 Transcription Factor Regulates Osteogenic Differentiation of Dental Pulp Stem Cells [0.03%]
转录因子MEF2通过调节牙髓干细胞成骨分化参与内源性牙齿修复
Shuling Shen,Dan Huang,Guijuan Feng et al.
Shuling Shen et al.
The myocyte enhancer factor-2 (MEF2) is a member of the MADS-box family. It controls the expression of genes that are critical for biological processes such as proliferation, cell death, and differentiation. Some studies have shown that MEF...
MicroRNA-145 Inhibitor Significantly Improves the Development of Bovine Somatic Cell Nuclear Transfer Embryos In Vitro [0.03%]
微RNA-145抑制剂可显著改善体细胞核移植胚胎的体外发育
Wenzhe Li,Yongjie Xiong,Fengyu Wang et al.
Wenzhe Li et al.
Directly regulating the translation of POU5F1, SOX2, KLF4, and miRNA-145 plays an important role in maintaining the pluripotency of stem cells and the development of early embryos. In the present study, the expression model of miRNA-145 on ...
PDE4 Inhibition by Rolipram Promotes Neuronal Differentiation in Human Bone Marrow Mesenchymal Stem Cells [0.03%]
罗吡溴铵通过抑制PDE4促进人骨髓间充质干细胞向神经元分化
I-Seul Joe,Goang-Won Cho
I-Seul Joe
Increased intracellular cyclic adenosine monophosphate (cAMP) can promote axonal elongation and facilitate neuronal repair, while decreased cAMP is associated with losses in neuronal regenerative capacity. Rolipram, which upregulates intrac...
Gnotobiotic Miniature Pig Interbreed Somatic Cell Nuclear Transfer for Xenotransplantation [0.03%]
无菌环境下异种（猪）嵌合体培养细胞核移植研究用于器官移植
Jeong Ho Hwang,Sang Eun Kim,Mukesh Kumar Gupta et al.
Jeong Ho Hwang et al.
Transgenic animal producing technology has improved consistently over the last couple of decades. Among the available methods, somatic cell nuclear transfer (SCNT) technology was officially the most popular. However, SCNT has low efficiency...
Developmental Outcome and Related Abnormalities in Goats: Comparison Between Somatic Cell Nuclear Transfer- and In Vivo-Derived Concepti During Pregnancy Through Term [0.03%]
体细胞核移植和体内受精山羊的孕期及产后相关异常及发育结局的比较研究
Leonardo Tondello Martins,Saul Gaudêncio Neto,Kaio César Simiano Tavares et al.
Leonardo Tondello Martins et al.
Cloning by somatic cell nuclear transfer (SCNT) is characterized by low efficiency and the occurrence of developmental abnormalities, which are rather poorly studied phenomena in goats. This study aimed at comparing overall SCNT efficiency ...
Generation of Induced Pluripotent Stem Cells from Diabetic Foot Ulcer Fibroblasts Using a Nonintegrative Sendai Virus [0.03%]
使用非整合性仙台病毒从糖尿病足溃疡纤维母细胞诱导生成多能干细胞
Behzad Gerami-Naini,Avi Smith,Anna G Maione et al.
Behzad Gerami-Naini et al.
Diabetic foot ulcers (DFUs) are nonhealing chronic wounds that are a serious complication of diabetes. Since induced pluripotent stem cells (iPSCs) may offer a potent source of autologous cells to heal these wounds, we studied if repair-def...
In Vitro Ectopic Behavior of Porcine Spermatogonial Germ Cells and Testicular Somatic Cells [0.03%]
猪精原生殖细胞和睾丸体细胞的离体异位行为研究
Kyung Hoon Lee,Won Young Lee,Jung Tae Do et al.
Kyung Hoon Lee et al.
Embryonic body-like colony formation is a unique pattern in male germ cell cultures, including spermatogonial stem cells. However, detailed information of the colony formation has not yet been sufficiently reported in male germ cell culture...
Effect of Cell Cycle Interactions and Inhibition of Histone Deacetylases on Development of Porcine Embryos Produced by Nuclear Transfer [0.03%]
细胞周期相互作用和组蛋白去乙酰化酶抑制对体细胞核移植胚胎发育的影响
Vitor B Rissi,Werner G Glanzner,Lady K S Mujica et al.
Vitor B Rissi et al.
The aim of this study was to evaluate if the positive effects of inhibiting histone deacetylase enzymes on cell reprogramming and development of somatic cell nuclear transfer (SCNT) embryos is affected by the cell cycle stage of nuclear don...
Cell Synchronization by Rapamycin Improves the Developmental Competence of Porcine SCNT Embryos [0.03%]
雷帕霉素同步细胞改善克隆胚胎发育潜能
Hyuk Hyun,Seung-Eun Lee,Yeo-Jin Son et al.
Hyuk Hyun et al.
The cell cycle stage of donor cells influences the success of somatic cell nuclear transfer (SCNT). This study investigated the effects of rapamycin treatment on synchronization of porcine fibroblasts in comparison with control and serum-st...