Valproic Acid Increases Histone Acetylation and Alters Gene Expression in the Donor Cells But Does Not Improve the In Vitro Developmental Competence of Buffalo (Bubalus bubalis) Embryos Produced by Hand-Made Cloning [0.03%]
伏立康唑增加供体细胞组蛋白乙酰化及基因表达但不能改善水牛(Bubalus bubalis)手搓克隆胚胎的发育潜能
Naresh L Selokar,Monika Saini,Himanshu Agrawal et al.
Naresh L Selokar et al.
Use of histone deacetylase inhibitors (HDACis) is believed to improve the developmental competence and quality of cloned embryos produced. We examined the effects of treatment of buffalo fibroblasts with valproic acid (VPA), a HDACi on thes...
Enrichment, Propagation, and Characterization of Mouse Testis-Derived Mesenchymal Stromal Cells [0.03%]
来源于小鼠睾丸的间充质干细胞的富集、扩增及鉴定
Mai Ahmed,Myret Ghabriel,Asma Amleh
Mai Ahmed
The therapeutic potential of multipotent stromal cells (MSCs) largely depends on the isolation and expansion methods used. In this study, we propose a laminin-based technique to select and enrich for MSCs isolated from the mouse testis. Pri...
Scriptaid Upregulates Expression of Development-Related Genes, Inhibits Apoptosis, and Improves the Development of Somatic Cell Nuclear Transfer Mini-Pig Embryos [0.03%]
scriptaid通过上调分化相关基因表达、抑制凋亡改善体细胞克隆小猪早期胚胎发育性能
Li Zhang,Yuemeng Huang,Yanjun Wu et al.
Li Zhang et al.
The present study was undertaken to investigate the mechanisms by which Scriptaid treatment improves the developmental competence of somatic cell nuclear transfer (SCNT) mini-pig embryos in vitro. We found that treatment with 500 nmol/L Scr...
Overexpression of Prox1 Induces the Differentiation of Human Adipose-Derived Stem Cells into Lymphatic Endothelial-Like Cells In Vitro [0.03%]
Prox1过表达诱导人脂肪来源干细胞体外分化为淋巴内皮样细胞
Jingcheng Deng,Tingting Dai,Yiyu Sun et al.
Jingcheng Deng et al.
Constant levels of homeobox transcription factor Prox1 expression are required throughout the life of lymphatic endothelial cells (LECs) to maintain their differentiated identity. Recent studies have demonstrated that using human LECs for c...
Differentiation of Mouse Ovarian Stem Cells Toward Oocyte-Like Structure by Coculture with Granulosa Cells [0.03%]
小鼠卵巢干细胞体外诱导分化为卵母细胞样结构
Soraya Parvari,Hossein Yazdekhasti,Zahra Rajabi et al.
Soraya Parvari et al.
An increasing body of evidence has confirmed existence and function of ovarian stem cells (OSCs). In this study, a novel approach on differentiation of OSCs into oocyte-like cells (OLCs) has been addressed. Recently, different methods have ...
Neural Differentiation of Mesenchymal Stem Cells on Scaffolds for Nerve Tissue Engineering Applications [0.03%]
用于神经组织工程的基底上间充质干细胞向神经细胞分化
Kerlin Quintiliano,Thayane Crestani,Davi Silveira et al.
Kerlin Quintiliano et al.
Scaffolds produced by electrospinning act as supports for cell proliferation and differentiation, improved through the release of neurotrophic factors. The objective of this study was to develop aligned and random nanofiber scaffolds with a...
Birth of Cloned Microminipigs Derived from Somatic Cell Nuclear Transfer Embryos That Have Been Transiently Treated with Valproic Acid [0.03%]
短时丙戊酸处理体细胞核移植胚胎可获得克隆微小型猪
Kazuchika Miyoshi,Hiroaki Kawaguchi,Kosuke Maeda et al.
Kazuchika Miyoshi et al.
In our previous study, we found that treatment of miniature pig somatic cell nuclear transfer (SCNT) embryos with 4 mM valproic acid (VPA), a histone deacetylase inhibitor, for 48 hours after activation enhanced blastocyst formation rate an...
Effects of the Transforming Growth Factor Beta Signaling Pathway on the Differentiation of Chicken Embryonic Stem Cells into Male Germ Cells [0.03%]
转化生长因子β信号通路对鸡胚胎干细胞向雄性生殖细胞分化影响的研究
Yani Zhang,Yingjie Wang,Qisheng Zuo et al.
Yani Zhang et al.
The objectives of the present study were to screen for key gene and signaling pathways involved in the production of male germ cells in poultry and to investigate the effects of the transforming growth factor beta (TGF-β) signaling pathway...
Isolation of Bovine Skin-Derived Precursor Cells and Their Developmental Potential After Nuclear Transfer [0.03%]
牛皮肤来源前体细胞的分离及其核移植发育潜能研究
Jiajia Xiao,Qiaoqiao Li,Pengxiang Qu et al.
Jiajia Xiao et al.
Nuclei from less differentiated stem cells yield high cloning efficiency. However, pluripotent stem cells are rather difficult to obtain from bovines. Skin-derived precursor (SKPs) cells exhibit a certain degree of pluripotency, which has b...
Increased Telomerase Reverse Transcriptase Expression Associates with Spontaneous Exit from M-II Arrest in Rat Eggs [0.03%]
端粒酶逆转录酶表达增强与受精前卵母细胞M二期阻滞退出增多有关
Shilpa Prasad,Shail K Chaube
Shilpa Prasad
In mammals, postovulatory egg aging deteriorates egg quality possibly by mediating spontaneous exit from metaphase-II (M-II) arrest and/or inducing apoptosis. To test this possibility, present study was designed to investigate telomerase re...