Hematopoietic Developmental Potential of Human Pluripotent Stem Cell Lines Is Accompanied by the Morphology of Embryoid Bodies and the Expression of Endodermal and Hematopoietic Markers [0.03%]
人多能干细胞系的造血发育潜能伴随胚体形态和内、造血标志物表达
Lenka Tesarova,Pavel Simara,Stanislav Stejskal et al.
Lenka Tesarova et al.
The potential clinical applications of hematopoietic stem cells (HSCs) derived from human pluripotent stem cells (hPSCs) are limited by the difficulty of recapitulating embryoid hematopoiesis and by the unknown differentiation potential of ...
Kai Xiong,Yan Zhou,Kristian Aabo Blichfeld et al.
Kai Xiong et al.
Specific activation of endogenous genes can be achieved by programmable artificial transcription factors (ATFs). In this study, we compared two artificial, programmable, clustered regularly interspaced short palindromic repeats (CRISPR)-bas...
Developmental Competence and Epigenetic Profile of Porcine Embryos Produced by Two Different Cloning Methods [0.03%]
两种不同克隆方法产生猪胚胎的发育潜能及表观遗传学特征比较研究
Ying Liu,Andrea Lucas-Hahn,Bjoern Petersen et al.
Ying Liu et al.
The "Dolly" based cloning (classical nuclear transfer, [CNT]) and the handmade cloning (HMC) are methods that are nowadays routinely used for somatic cloning of large domestic species. Both cloning protocols share several similarities, but ...
Mesenchymal Stem/Stromal Cells in Regenerative Medicine: Past, Present, and Future [0.03%]
再生医学中的间充质干细胞/基质细胞：过去、现在与未来
Ourania Trohatou,Maria G Roubelakis
Ourania Trohatou
The concept of Regenerative Medicine combined with Cell based Therapy and Tissue Engineering represents the fourth pillar of healthcare and provides a promising approach for the treatment of serious diseases. Recently, cell based therapies ...
Treatment of Donor Cells and Reconstructed Embryos with a Combination of Trichostatin-A and 5-aza-2'-Deoxycytidine Improves the Developmental Competence and Quality of Buffalo Embryos Produced by Handmade Cloning and Alters Their Epigenetic Status and Gene Expression [0.03%]
曲古茋素A和5-aza-2'-脱氧胞苷联合处理改善水牛体细胞克隆胚胎发育潜能及重塑其表观修饰状态和基因表达谱式
Monika Saini,Naresh L Selokar,Himanshu Agrawal et al.
Monika Saini et al.
The application of cloning technology on a large scale is limited by very low offspring rate primarily due to aberrant or incomplete epigenetic reprogramming. Trichostatin A (TSA), a histone deacetylase inhibitor, and 5-aza-2'-deoxycytidine...
Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro [0.03%]
体外长期培养下牙龈间充质干细胞中癌基因表达的重编程
Agnese Gugliandolo,Thangavelu Soundara Rajan,Domenico Scionti et al.
Agnese Gugliandolo et al.
Mesenchymal stem cells (MSCs) are a promising resource for stem cell therapy for the treatment of different neurodegenerative disorders. In particular, dental MSCs, given their origin from neural crest and their proneness toward neuronal di...
Improved Isolation, Proliferation, and Differentiation Capacity of Mouse Ovarian Putative Stem Cells [0.03%]
小鼠卵巢干细胞的分离、增殖及分化能力的改善
Hossein Yazdekhasti,Marzieh Agha Hosseini,Zahra Rajabi et al.
Hossein Yazdekhasti et al.
The recent discovery of ovarian stem cells in postnatal mammalian ovaries, also referred to as putative stem cells (PSCs), and their roles in mammalian fertility has challenged the long-existing theory that women are endowed with a certain ...
Epigenetic Status of H19-Igf2 Imprinted Genes and Loss of 5-Hydroxymethylcytosine in the Brain of Cloned Goats [0.03%]
克隆山羊脑中H19-Igf2印迹基因的表观遗传状态及5-羟甲基胞嘧啶丢失
Mingtian Deng,Caifang Ren,Zifei Liu et al.
Mingtian Deng et al.
In mammals, the imprinted genes play vital roles in development and are generally controlled by DNA methylation at imprinting control regions (ICRs). Recently, it was discovered that 5-hydroxymethylcytosine (5-hmC) is a stable epigenetic mo...
Genetic Manipulation by Zinc-Finger Nucleases in Rat-Induced Pluripotent Stem Cells [0.03%]
锌指核酸酶基因打靶技术用于诱导多能干细胞研究
Sheng Yang,Shufang Ding,Qianhua Xu et al.
Sheng Yang et al.
Induced pluripotent stem cells (iPSCs) have an extensive application in regenerative medicine, pharmaceutical discovery, and basic research. With the recent derivation of rat iPSCs, it is now feasible to apply genetic manipulation in this s...
Regulation of Embryonic Stem Cell Self-Renewal and Differentiation by MicroRNAs [0.03%]
微小核糖核酸对胚胎干细胞自我更新和分化的调控作用
Xi Ran,Chun-Hong Xiao,Gui-Ming Xiang et al.
Xi Ran et al.
MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression. They play an important role in various cellular processes such as apoptosis, differentiation, secretion, and proliferation. Embryonic stem cells (ESCs) are derived fr...