Evaluating the Effect of Cell Culture on Gene Expression in Primary Tissue Samples Using Microfluidic-Based Single Cell Transcriptional Analysis [0.03%]
基于微流控的单细胞转录组分析评估体外培养对组织样本基因表达的影响
Michael Januszyk,Robert C Rennert,Michael Sorkin et al.
Michael Januszyk et al.
Significant transcriptional heterogeneity is an inherent property of complex tissues such as tumors and healing wounds. Traditional methods of high-throughput analysis rely on pooling gene expression data from hundreds of thousands of cells...
Analysis of Reverse Phase Protein Array Data: From Experimental Design towards Targeted Biomarker Discovery [0.03%]
反向蛋白质印迹阵列数据分析：从实验设计到目标生物标志物的发现
Astrid Wachter,Stephan Bernhardt,Tim Beissbarth et al.
Astrid Wachter et al.
Mastering the systematic analysis of tumor tissues on a large scale has long been a technical challenge for proteomics. In 2001, reverse phase protein arrays (RPPA) were added to the repertoire of existing immunoassays, which, for the first...
Cancer-Osteoblast Interaction Reduces Sost Expression in Osteoblasts and Up-Regulates lncRNA MALAT1 in Prostate Cancer [0.03%]
前列腺癌细胞与成骨细胞相互作用通过降低SOST的表达和上调长链非编码RNA MALAT1影响成骨细胞功能
Aimy Sebastian,Nicholas R Hum,Bryan D Hudson et al.
Aimy Sebastian et al.
Dynamic interaction between prostate cancer and the bone microenvironment is a major contributor to metastasis of prostate cancer to bone. In this study, we utilized an in vitro co-culture model of PC3 prostate cancer cells and osteoblasts ...
SNP Analysis and Whole Exome Sequencing: Their Application in the Analysis of a Consanguineous Pedigree Segregating Ataxia [0.03%]
应用于共济运动障碍谱系的连锁分析及全外显子组测序研究
Sarah L Nickerson,Renate Marquis-Nicholson,Karen Claxton et al.
Sarah L Nickerson et al.
Autosomal recessive cerebellar ataxia encompasses a large and heterogeneous group of neurodegenerative disorders. We employed single nucleotide polymorphism (SNP) analysis and whole exome sequencing to investigate a consanguineous Maori ped...
Integrated Microfluidic Nucleic Acid Isolation, Isothermal Amplification, and Amplicon Quantification [0.03%]
集成为微流体核酸隔离、等温放大和放大基因定量于一体的设备
Michael G Mauk,Changchun Liu,Jinzhao Song et al.
Michael G Mauk et al.
Microfluidic components and systems for rapid (
Kenichi Inaoka,Yoshikuni Inokawa,Shuji Nomoto
Kenichi Inaoka
DNA microarray technologies have advanced rapidly and had a profound impact on examining gene expression on a genomic scale in research. This review discusses the history and development of microarray and DNA chip devices, and specific micr...
Farhad Maleki,Anthony Kusalik
Farhad Maleki
Cellular pathways involve the phosphorylation and dephosphorylation of proteins. Peptide microarrays called kinome arrays facilitate the measurement of the phosphorylation activity of hundreds of proteins in a single experiment. Analyzing t...
Dania Albaba,Sanam Soomro,Chandra Mohan
Dania Albaba
In recent years, aptamers have come to replace antibodies in high throughput multiplexed experiments. The aptamer-based biomarker screening technology, which kicked off in 2010, is capable of interrogating thousands of proteins in a very sm...
Logan C Walker,George A R Wiggins,John F Pearson
Logan C Walker
Constitutional copy number variants (CNVs) include inherited and de novo deviations from a diploid state at a defined genomic region. These variants contribute significantly to genetic variation and disease in humans, including breast cance...
Microarray Meta-Analysis and Cross-Platform Normalization: Integrative Genomics for Robust Biomarker Discovery [0.03%]
基因芯片的元分析和跨平台标准化：用于可靠生物标志物发现的整体基因组学
Christopher J Walsh,Pingzhao Hu,Jane Batt et al.
Christopher J Walsh et al.
The diagnostic and prognostic potential of the vast quantity of publicly-available microarray data has driven the development of methods for integrating the data from different microarray platforms. Cross-platform integration, when appropri...