Tissue Microarray Technology for Molecular Applications: Investigation of Cross-Contamination between Tissue Samples Obtained from the Same Punching Device [0.03%]
组织微阵列技术在分子生物学中的应用及来自同一取芯装置的样本之间的交叉污染研究
Erik Vassella,José A Galván,Inti Zlobec
Erik Vassella
Background: Tissue microarray (TMA) technology allows rapid visualization of molecular markers by immunohistochemistry and in situ hybridization. In addition, TMA instrumentation has the potential to assist in other appli...
Immune-Signatures for Lung Cancer Diagnostics: Evaluation of Protein Microarray Data Normalization Strategies [0.03%]
用于肺癌诊断的免疫标志物：蛋白质微阵列数据归一化策略的评价
Stefanie Brezina,Regina Soldo,Roman Kreuzhuber et al.
Stefanie Brezina et al.
New minimal invasive diagnostic methods for early detection of lung cancer are urgently needed. It is known that the immune system responds to tumors with production of tumor-autoantibodies. Protein microarrays are a suitable highly multipl...
Therese Andersen,Pia Auk-Emblem,Michael Dornish
Therese Andersen
This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alg...
Martin Witt,Johanna-Gabriela Walter,Frank Stahl
Martin Witt
Microarray technologies are state of the art in biological research, which requires fast genome, proteome and transcriptome analysis technologies. Often antibodies are applied in protein microarrays as proteomic tools. Since the generation ...
Reverse Phase Protein Arrays-Quantitative Assessment of Multiple Biomarkers in Biopsies for Clinical Use [0.03%]
反向蛋白质阵列技术——在临床活检中量化多种生物标志物
Stefanie Boellner,Karl-Friedrich Becker
Stefanie Boellner
Reverse Phase Protein Arrays (RPPA) represent a very promising sensitive and precise high-throughput technology for the quantitative measurement of hundreds of signaling proteins in biological and clinical samples. This array format allows ...
In Silico Genomic Fingerprints of the Bacillus anthracis Group Obtained by Virtual Hybridization [0.03%]
克里米亚刚果出血热病毒糖蛋白单克隆抗体的鉴定与表征
Hueman Jaimes-Díaz,Violeta Larios-Serrato,Teresa Lloret-Sánchez et al.
Hueman Jaimes-Díaz et al.
In this study we evaluate the capacity of Virtual Hybridization to identify between highly related bacterial strains. Eight genomic fingerprints were obtained by virtual hybridization for the Bacillus anthracis genome set, and a set of 15,2...
Anastasia Bachmann,Matthias Moll,Eric Gottwald et al.
Anastasia Bachmann et al.
One of the main challenges in drug development is the prediction of in vivo toxicity based on in vitro data. The standard cultivation system for primary human hepatocytes is based on monolayer cultures, even if it is known that these condit...
Gold Nanoparticles Surface Plasmon Resonance Enhanced Signal for the Detection of Small Molecules on Split-Aptamer Microarrays (Small Molecules Detection from Split-Aptamers) [0.03%]
金纳米粒子表面等离子共振增强分裂型适配体微阵列检测小分子信号
Feriel Melaine,Yoann Roupioz,Arnaud Buhot
Feriel Melaine
The detection of small molecules by biosensors remains a challenge for diagnostics in many areas like pharmacology, environment or homeland security. The main difficulty comes from both the low molecular weight and low concentrations of mos...
Transcriptome Analysis of Porphyrin-Accumulated and X-Ray-Irradiated Cell Cultures under Limited Proliferation and Non-Lethal Conditions [0.03%]
有限增殖和非致死条件下细胞培养物中卟啉累积和X射线辐照的转录组分析
Junko Takahashi,Masaki Misawa,Hitoshi Iwahashi
Junko Takahashi
5-Aminolevulinic acid (ALA) is a precursor of the photosensitizer used in photodynamic therapy. It accumulates in tumor cells and subsequently metabolizes to protoporphyrin IX (PpIX), which generates singlet oxygen after light irradiation. ...
Unraveling the Specific Ischemic Core and Penumbra Transcriptome in the Permanent Middle Cerebral Artery Occlusion Mouse Model Brain Treated with the Neuropeptide PACAP38 [0.03%]
PACAP38治疗永久性大脑中动脉闭塞模型脑缺血半暗带和梗死核心区转录组的差异响应研究
Motohide Hori,Tomoya Nakamachi,Junko Shibato et al.
Motohide Hori et al.
Our group has been systematically investigating the effects of the neuropeptide pituitary adenylate-cyclase activating polypeptide (PACAP) on the ischemic brain. To do so, we have established and utilized the permanent middle cerebral arter...