Development of a Chicken Immunoglobulin Heavy Chain Variable Region (VH) Single-Domain Antibody (sdAb) Against Calsequestrin (CSQ) and Its Application [0.03%]
一种抗鸡肌浆网钙处理蛋白(CSQ)的单域抗体(VHH)及其应用
Sun Lee,Seoryeong Park,Hyunji Yang et al.
Sun Lee et al.
Background/Objectives: Calsequestrin (CSQ) is a calcium-binding protein that is highly soluble and can serve as a solubility-enhancing fusion tag in recombinant protein expression. Its unique property of calcium-induced precipitation follow...
Monoclonal Antibodies and Small-Molecule Therapies for Lichen Planus: Targeted Immunomodulation and Emerging Evidence [0.03%]
用于扁平苔藓的单克隆抗体和小分子疗法：靶向免疫调节及新证据
Francois Rosset,Nadia Sciamarrelli,Luca Mastorino et al.
Francois Rosset et al.
Background/Objectives: Lichen planus (LP) is a chronic inflammatory disease of autoimmune origin, affecting the skin and mucous membranes. While corticosteroids and immunosuppressants are traditionally used, many cases remain refractory or ...
Masayuki Tasaki,Kazuhide Saito,Kota Takahashi
Masayuki Tasaki
Background: The accurate evaluation of anti-ABO antibodies is essential for risk stratification in ABO-incompatible (ABOi) transplantation. Historically, hemagglutination-based titration has been the cornerstone of such an assessment; howev...
Comparative Evaluation of Three Primary Antibody Clones for p16 Immunohistochemistry in Gynecologic Tumors [0.03%]
用于妇科肿瘤p16免疫组化三种单克隆抗体的比较评估研究
Hiroshi Yoshida,Ayumi Sugitani,Mayumi Kobayashi-Kato et al.
Hiroshi Yoshida et al.
Background: p16 immunohistochemistry (IHC) serves as a surrogate marker for high-risk human papillomavirus (hrHPV) and is widely used in gynecologic pathology. However, few studies have directly compared the staining perf...
Brigita Šemeklienė,Brigita Gradauskienė
Brigita Šemeklienė
Infertility is a multifactorial condition with a wide range of potential causes, including anatomical, hormonal, genetic, and lifestyle-related factors. Among these, immunological mechanisms have increasingly been recognized as important co...
Safety and Effectiveness of Dupilumab in Atopic Dermatitis Patients with Hematologic Comorbidities: A Multicenter, Retrospective Study [0.03%]
Dupilumab治疗血液系统疾病合并特应性皮炎患者的疗效和安全性：多中心回顾性研究
Luca Bettolini,Stefano Bighetti,Silvia Mariel Ferrucci et al.
Luca Bettolini et al.
Background: Dupilumab, a monoclonal antibody targeting the interleukin-4 receptor α, is approved for moderate-to-severe atopic dermatitis (AD). However, its safety profile in patients with concomitant hematologic disorde...
Structure-Guided Stapling of Dimeric Conformations and Linker Engineering Enhance Thermostability and Fine-Tune Activity of Bispecific VHH Cytokine Agonists [0.03%]
结构指导的二聚体构象及连接臂工程化稳定了双特异纳米抗体类细胞因子的热稳定性并调控其活性
Raphael Trenker,Deepti Rokkam,Andrew Morin et al.
Raphael Trenker et al.
Background: Bispecific antibodies have emerged as a promising class of therapeutics, enabling simultaneous targeting of two distinct antigens. Single-domain antibodies (sdAbs) comprising camelid variable heavy chains (VHH...
Computational Prediction of Single-Domain Immunoglobulin Aggregation Propensities Facilitates Discovery and Humanization of Recombinant Nanobodies [0.03%]
计算预测单结构域免疫球蛋白聚集倾向可促进重组纳米抗体的发现和人源化改造
Felix Klaus Geyer,Julian Borbeck,Wiktoria Palka et al.
Felix Klaus Geyer et al.
Background/objectives: Single-domain immunoglobulins are small protein modules with specific affinities. Among them, the variable domains of heavy chains of heavy-chain-only antibodies (VHH) as the antigen-binding fragmen...
Cost-Effective Method for Full-Length Sequencing of Monoclonal Antibodies from Hybridoma Cells [0.03%]
一种来自杂交瘤细胞的单克隆抗体全长测序的经济方法
Sarah Döring,Georg Tscheuschner,Sabine Flemig et al.
Sarah Döring et al.
Background: Monoclonal antibodies play an important role in therapeutic and analytical applications. For recombinant expression, the coding sequences of the variable regions of the heavy and light chains are required. In ...
Guidelines in the Preparation of Fully Synthetic, Human Single-Domain Antibody Phage Display Libraries [0.03%]
关于全人工合成的人源化单域抗体噬菌体展示文库构建的指导原则
Mark A Tornetta,Brian P Whitaker,Olivia M Cantwell et al.
Mark A Tornetta et al.
Background/objectives: The complexity of diseases such as cancer and auto-immune disorders drives the need for unique, target-driven therapeutics. A broader arsenal to generate better biologics-based therapeutics is neede...