Room-temperature crystal structure of a metal-dependent W-formate dehydrogenase by serial synchrotron crystallography [0.03%]
基于同步辐射连续晶体学的金属依赖型W甲酸脱氢酶室温下晶体结构解析
Guilherme Vilela-Alves,Guilherme Martins,David von Stetten et al.
Guilherme Vilela-Alves et al.
Metal-dependent formate dehydrogenases (Fdhs) are a promising research target in efforts to mitigate climate change by developing active, efficient, selective and safe industrial catalysts for CO2 reduction. Here, we report the room-tempera...
Influence of heme and pH on the oligomeric states of Brucella melitensis bacterioferritin [0.03%]
血红素和pH值对布鲁氏菌分泌铁蛋白寡聚状态的影响
Tanguy Scaillet,Clément Champagne,Johan Wouters
Tanguy Scaillet
Bacterioferritin from Brucella melitensis (BmBfr) is a protein that plays essential roles in oxidative stress management and metal homeostasis in the bacterium. It presents itself as a 24-mer forming a spherical nanocage, allowing further a...
Replacement soaking for human tankyrase 2 enables studies on substrate analogues and inhibitors [0.03%]
人Tankyrase 2的替代浸泡使底物类似物和抑制剂的研究成为可能
Johan Pääkkönen,Sven T Sowa,Chiara Bosetti et al.
Johan Pääkkönen et al.
Crystallography has facilitated the development of inhibitors in modern drug discovery. Complex structures are traditionally obtained by co-crystallization or soaking methods, but they are not always successful; co-crystallization may lead ...
Advances in the nanostructure characterization of biological hydrogels formed by the prion-like domain of EARLY FLOWERING 3 [0.03%]
EARLY FLOWERING 3 类朊蛋白结构域形成生物水凝胶的纳米结构表征研究取得进展
Stephanie Hutin,Pedro L O Filho,Anton M Popov et al.
Stephanie Hutin et al.
Hydrogels exhibit traits of both liquids and solids, forming a 3D structural network with generally high water content, high porosity and increasing stability over time. However, probing the structure of hydrogels is challenging due to the ...
An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM [0.03%]
一种无人值守的图像处理流程，用于低温电子显微镜(Cryo-EM)中的实时质量评估和三维探索
Daniel Marchán Torres,Pablo Conesa,Alberto Garcia et al.
Daniel Marchán Torres et al.
Single-particle analysis (SPA) by cryogenic electron microscopy (cryo-EM) has become a cornerstone of structural biology; yet, the workflow from data acquisition to a 3D structure remains a time-consuming, manual and significant task. Resea...
Dose-dependent structural and electron-density features in the lytic polysaccharide monooxygenase NcAA9D [0.03%]
剂量依赖的结构和电子密度特征在裂解多糖单加氧酶NcAA9D中
Samuel A Miller,William B O&#x;Dell,Flora Meilleur
Samuel A Miller
Structural studies of copper-containing lytic polysaccharide monooxygenases (LPMOs) by X-ray crystallography are often complicated by radiation damage. In this study, we analyze a series of 36 X-ray crystal structures of NcAA9D, a Neurospor...
LowDoseWizard: rapid and standardized setup of low-dose cryo-TEM imaging in SerialEM [0.03%]
低剂量漂移校正的冷冻电子断层扫描自动化软件LowDoseWizard
Simon A Fromm,Simone Mattei
Simon A Fromm
Structure elucidation of biological macromolecules by single-particle cryogenic electron microscopy (SPA cryo-EM) or cryogenic electron tomography (cryo-ET) relies on low-dose imaging on cryogenic transmission electron microscopes (cryo-TEM...
Crystal structure of pilus-specific sortase from Enterococcus faecalis reveals both open and closed conformations: insights into loop dynamics and substrate recognition [0.03%]
肠球菌属粪肠球菌特异性菌毛连接酶的晶体结构揭示了其既具开放又具封闭构型：环域动态及底物识别的新见解
Vinay Sharma,Sumit Murmu,Rajendra Prasad Roy et al.
Vinay Sharma et al.
Pilus-specific or class C sortases (SrtCs) catalyze pilus polymerization in Gram-positive bacteria and possess a critical lid near the active site that is in a closed or open conformation. The crystal structure of SrtC, which polymerizes en...
Structural basis for the fast maturation of pcStar, a photoconvertible fluorescent protein. Erratum [0.03%]
有关快速成熟的光转化荧光蛋白pcStar的结构基础的勘误页
Shuping Zheng,Xiangrui Shi,Junjin Lin et al.
Shuping Zheng et al.
Several corrections are made to the article by Zheng et al. [(2025), Acta Cryst. D81, 181-195]. Keywords: cr...
Crystal structure of endo-β-1,6-galactanase from Streptomyces avermitilis [0.03%]
链霉菌芽胞菌内-β-1,6-半乳聚糖酶的晶体结构
Zui Fujimoto,Naomi Kishine,Toshihisa Kotake et al.
Zui Fujimoto et al.
Endo-β-1,6-galactanases hydrolyze β-1,6-linked galactosyl linkages in galactans, yielding β-1,6-linked galacto-oligosaccharides, predominantly galactobiose. Here, we report to our knowledge the first crystal structure of an endo-β-1,6-g...