Genetic engineering of Staphylococcus haemolyticus: overcoming restriction-modification barriers and targeting virulence genes [0.03%]
金黄色葡萄球菌基因工程：克服限制修饰障碍并靶向毒力基因
Hermoine J Venter,J Pauline Cavanagh,Runa Wolden et al.
Hermoine J Venter et al.
Staphylococcus haemolyticus is an emerging multidrug-resistant nosocomial pathogen noted for robust biofilm formation and complex restriction-modification (RM) systems that hinder genetic manipulation. These barriers have severely limited m...
Novel transposon Tn 8026 contributes to the global spread of transmissible linezolid resistance in Enterococcus via a linear plasmid [0.03%]
新型转座子Tn8026通过线性质粒促进可传播的利奈唑胺耐药肠球菌的全球流行
Michael B Hall,Yiyang Xue,Tricia S E Lee et al.
Michael B Hall et al.
Linezolid is a critical last-resort antimicrobial for multidrug-resistant Enterococcus faecium, particularly against vancomycin-resistant lineages where therapeutic options are severely limited. While resistance has historically arisen thro...
Performance of mechanically sheared DNA in multiplexed Oxford Nanopore sequencing for Salmonella Typhi genomic surveillance [0.03%]
机械剪切DNA在用于伤寒沙门氏菌基因组监测的牛津纳米孔测序平台多重检测中的性能评估
Hsu Thinzar Maung,Masamoto Morita,Su Myat Han et al.
Hsu Thinzar Maung et al.
Pathogen genomic surveillance is essential for monitoring high-risk lineages and antimicrobial resistance in Salmonella enterica serovar Typhi (S. Typhi), yet implementing whole-genome sequencing in sentinel hospital laboratories remains ch...
In silico and experimental assessment of the 16S-ITS-23S operon as a genetic marker for high-resolution bacterial species identification [0.03%]
基于16S-ITS-23S操纵子的高分辨细菌物种鉴定的计算和实验评估研究
Ana C Yamakawa,Matthew Davies,Hyun S Gweon
Ana C Yamakawa
Accurate species-level identification of bacteria is crucial for public health surveillance, but standard methods like 16S rRNA hypervariable region (HVR) sequencing often lack sufficient resolution. This study systematically evaluates the ...
Molecular epidemiology reveals distinct lineages and genomic profiles of avian pathogenic and commensal Escherichia coli in broiler flocks [0.03%]
分子流行病学揭示了肉鸡群中致病性和共生性大肠杆菌的不同谱系和基因组特征
Homayoon Davam,Désirée S Jansson,Emma Nord et al.
Homayoon Davam et al.
Escherichia coli is predominantly an intestinal commensal; however, avian pathogenic E. coli (APEC) causes colibacillosis in poultry. The APEC pathotype lacks a clear genetic definition, further complicated by its opportunistic nature. To c...
Home is where the host is: evolutionary history of geographic spread, host switching and adaptive genomic signatures in two generalist group B Streptococcus clonal group [0.03%]
家园有主：B群链球菌两克隆群体的地理扩散、宿主转换及适应性基因组特征的进化历史
Antonia Hilbig,Chiara Crestani,Timothy Barkham et al.
Antonia Hilbig et al.
Group B Streptococcus (GBS) is a pathogen of global relevance in neonatal and maternal disease as well as bovine mastitis. Two closely related clonal group, denoted 103 and 314 (CG103/314), have been detected in humans and cattle on multipl...
Mobile genetic element diversity across Shiga toxin-producing Escherichia coli lineages in French cattle [0.03%]
法国牛源志贺毒素产生大肠杆菌插入序列和质粒多样性分析
Fabien Vorimore,Mai-Lan Tran,Sandra Jaudou et al.
Fabien Vorimore et al.
Shiga toxin-producing Escherichia coli (STEC) represent a genetically diverse group of pathogens whose virulence is largely driven by mobile genetic elements (MGEs), including plasmids and bacteriophages. While horizontal gene transfer is c...
The pQBR mercury resistance plasmids: a model set of sympatric environmental mobile genetic elements [0.03%]
同域环境中的可移动基因元件模型集：pQBR汞抗性质粒
Victoria T Orr,Ellie Harrison,Damian W Rivett et al.
Victoria T Orr et al.
Plasmids are extrachromosomal mobile genetic elements that can facilitate rapid bacterial adaptation by transferring genes between individuals. Whilst plasmids are known to exist in diverse habitats and encode a range of traits, most of our...
Salmonella bloodstream infection and concurrent intestinal colonization in children with severe acute malnutrition in Niger [0.03%]
尼日尔严重急性营养不良儿童的沙门氏菌血流感染和并发肠道定植
Kirsty Sands,Raúl Campillo,Ian Boostrom et al.
Kirsty Sands et al.
Children with severe acute malnutrition (SAM) are at high risk of invasive infection. In sub-Saharan Africa, invasive non-typhoidal Salmonella enterica (iNTS) serovars are a leading cause of paediatric bloodstream infection. However, low-re...
Genomic surveillance reveals long-term endemicity and outbreak potential of Klebsiella pneumoniae sequence type 48 in a German hospital and its global context [0.03%]
基因组监测揭示肺炎克雷伯菌序列型48在德国医院中的长期地方性和全球背景下的爆发潜能
Ngoc Minh Nguyen,Anna Weber,Basil Britto Xavier et al.
Ngoc Minh Nguyen et al.
Klebsiella pneumoniae sequence type 48 (Kp-ST48) is a globally distributed clone linked to antimicrobial resistance (AMR) yet lacks a comprehensive genomic analysis. Here, we investigated the persistence, transmission dynamics and global co...