Dorothee Liebschner,Michael A Hough,Stephen P Muench et al.
Dorothee Liebschner et al.
This editorial introduces Protocols, a new article category in Acta Crystallographica Section F designed to provide detailed, reproducible workflows for structural biology methods. The new format promotes transparency, reproducibility and t...
Structure of human aldehyde oxidase under tris(2-carboxyethyl)phosphine reducing conditions [0.03%]
还原条件下人的醛氧化酶结构
Carolina Videira,Mariam Esmaeeli,Silke Leimkühler et al.
Carolina Videira et al.
The importance of human aldehyde oxidase (hAOX1) has increased in recent decades due to its involvement in drug metabolism. Inhibition studies involving hAOX1 are extensive and a common reducing agent, dithiothreitol (DTT), was recently fou...
WebCalEM: a browser-based tool for routine and accurate pixel-size calibration in cryo-EM [0.03%]
WebCalEM：一个基于浏览器的工具，可实现cryo-EM中常规而精确的像素尺寸校准
Lan Dang,Zhiqing Wang,Sung Hyun Joseph Cho et al.
Lan Dang et al.
Accurate determination of the image pixel size is critical for quantitative cryo-electron microscopy analyses, yet existing calibration methods remain under-utilized because installation barriers and workflow complexity discourage routine a...
Crystal structure of Legionella pneumophila glycosidase effector LegY [0.03%]
Legionella pneumophila糖苷酶效应蛋白LegY的晶体结构
Jing Chen,Fan Li,Shao Yuan Wang et al.
Jing Chen et al.
Legionella pneumophila translocates approximately 330 effectors into host cells via its type IVB secretion system. These effectors mediate a diverse array of post-translational modifications, among which reversible glycosylation is closely ...
Crystal structure of human Dickkopf 4 cysteine-rich domain 1 and evaluation of conformational rigidity [0.03%]
人Dickkopf 4半胱氨酸富集结构域1的晶体结构及其构象rigid性的评估
Naoki Shibata
Naoki Shibata
Dickkopf (DKK) family proteins (DKK1-DKK4), which function as extracellular modulators of Wnt signaling, contain two cysteine-rich domains: CRD1 and CRD2. In DKK1, CRD1 modulates interaction with its receptor low-density lipoprotein recepto...
Crystal structure of the type VI secretion system adaptor protein Tla3 from Pseudomonas aeruginosa [0.03%]
铜绿假单胞菌VI型分泌系统适配蛋白Tla3的晶体结构
Bidisha Chakraborty,Saumen Datta
Bidisha Chakraborty
The type VI secretion system (T6SS) is a dynamic protein nanomachine employed by Gram-negative bacteria to secrete toxic effector proteins directly into microbial competitors, eukaryotic host cells, or the environment. The T6SS is primarily...
Jan Hannes Schaefer,Robert T O&#x;Neill,Joseph P Donnelly et al.
Jan Hannes Schaefer et al.
Amyloid-mediated proteotoxicity underlies over 50 diseases. Cryo-EM establishes direct links between filament morphologies and pathology, although microbial functional amyloids illustrate that this fold can evolve to serve physiological rol...
Crystal structure of the cytosolic Nudix-like domain of CD-NTase-associated protein 16 from Enterococcus faecalis [0.03%]
屎肠球菌CD-NTase相关蛋白16的胞质Nudix样结构域晶体结构
Tinashe Makanyire,Zihan Zhou,Wanqian Wu et al.
Tinashe Makanyire et al.
The cyclic oligonucleotide-based antiphage signaling system (CBASS) employs diverse effector proteins to trigger abortive infection upon phage invasion, yet structural information on membrane-associated CBASS effectors remains limited. CD-N...
Crystal structures of PCNA1 and PCNA2 from Aeropyrum pernix: implications for a distorted heterotrimeric sliding clamp [0.03%]
来自Aeropyrum pernix的PCNA1和PCNA2晶体结构及其异三聚体滑动夹的变形机制意义
Tong Wang,Sonoko Ishino,Yoshizumi Ishino et al.
Tong Wang et al.
Aeropyrum pernix is a hyperthermophilic archaeon that possesses three proliferating cell nuclear antigen (PCNA) isoforms (ApePCNA1, ApePCNA2 and ApePCNA3) that form a heterotrimeric sliding clamp. To gain more detailed structural insights i...
Structural analysis of the flexibility of the Ubl2 domain within the papain-like protease of SARS-CoV-2 [0.03%]
SARS-CoV-2的Papain样蛋白酶中的Ubl2结构域的灵活性的结构分析
Gian Luca Freiherr von Scholley,Martina Schaefer,Mark D Tully et al.
Gian Luca Freiherr von Scholley et al.
The papain-like protease (PLpro) of SARS-CoV-2 is part of the multi-domain nonstructural protein 3 (NSP3) and consists of two domains: a ubiquitin-like domain 2 (Ubl2) and a protease domain. PLpro plays a crucial role in the replication cyc...