Advances in cryo-EM that have shaped mechanistic models of membrane-attack-complex assembly and regulation [0.03%]
冷冻电子显微镜在膜攻击复合物组装和调节机制模型方面的发展进展
Dylan P Noone,Doryen Bubeck
Dylan P Noone
The complement system is a blood-based immune network that plays a crucial role in fighting infection and maintaining immune homeostasis. The membrane attack complex (MAC) is a pore assembled from complement proteins that creates holes in c...
3D atomic structure determination with ultrashort-pulse MeV electron diffraction [0.03%]
利用超短MeV电子衍射进行三维原子结构分析
Vincent Hennicke,Max Hachmann,Paul Benjamin Klar et al.
Vincent Hennicke et al.
Understanding structure at the atomic scale is fundamental for understanding the functioning and the development of materials with improved properties. Compared with other probes providing atomic resolution, electrons offer the strongest in...
High-throughput automated molecular replacement for small-molecule MicroED data [0.03%]
用于小分子MicroED数据的高通量自动化分子置换法
Adam Thibodeaux,Guanhong Bu,Lael C Edwards et al.
Adam Thibodeaux et al.
Interest in electron diffraction (ED) for structural characterization of both proteins and small molecules has grown significantly over the last decade. While ab initio phasing methods remain the gold standard for ED data from small-molecul...
Structural insights into MltB from Acinetobacter baumannii: a conserved catalytic tunnel with unique domain arrangement [0.03%]
鲍曼不动杆菌MltB的结构洞察：具有独特结构域排列的一条保守催化隧道
Hyunseok Jang,Chang Min Kim,Chang Sup Lee et al.
Hyunseok Jang et al.
Lytic transglycosylases (LTs) play a central role in bacterial cell wall remodeling by cleaving the β-1,4-glycosidic bond in peptidoglycan via a non-hydrolytic mechanism, producing 1,6-anhydroMurNAc termini crucial for recycling and signal...
Evaluating the Volta phase plate for improved tomogram alignment in cryo-electron tomography [0.03%]
评估Volta相板在低温电子断层扫描中的对齐效果改善作用
Joshua Hutchings,Daniel Ji,Shawn Zheng et al.
Joshua Hutchings et al.
The Volta phase plate (VPP) can enhance low-frequency phase contrast in cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET), but its impact on tomogram alignment has not been systematically assessed. Using sub-2 Å sin...
Probing laser-driven surface and subsurface dynamics via grazing-incidence XFEL scattering and diffraction [0.03%]
基于斜入射X射线自由电子激光散射和衍射的飞秒强激光驱动表/界面及次表面动力学探测研究
Lisa Randolph,Özgül Öztürk,Dmitriy Ksenzov et al.
Lisa Randolph et al.
We demonstrate a grazing-incidence X-ray platform that simultaneously records time-resolved grazing-incidence small-angle X-ray scattering (GISAXS) and grazing-incidence X-ray diffraction (GID) from a femtosecond-laser-irradiated gold film ...
AXIS: a Lab-in-the-Loop machine learning approach for automated detection of macromolecular crystals [0.03%]
轴线：一种用于自动检测大分子晶体的闭环机器学习方法
Aurelien Personnaz,Sihyun Sung,Raphael Bourgeas et al.
Aurelien Personnaz et al.
Macromolecular crystallography provides mechanistic understanding of biological processes and can be applied in drug design. Nowadays, the use of robotic systems for crystal growth and diffraction analysis is widespread and high-throughput ...
Stéphanie Kodjikian
Stéphanie Kodjikian
Now a common technique in crystallography, three-dimensional electron diffraction (3D ED) is on track to bridge the gap with X-ray diffraction in terms of structural accuracy. ...
Kinetic effects yield different results on the timescales of laboratory and synchrotron high-pressure experiments [0.03%]
力学效应导致实验室和同步辐射高压实验时间尺度上的结果不同
Julien Haines
Julien Haines
Kinetic effects can be a critical factor in the study of high-pressure phase transitions. X-ray diffraction experiments on the timescales of the laboratory and the synchrotron can provide complementary results on such transformations. ...
Jon Cooper
Jon Cooper
The paper by Gilski et al. in this issue [IUCrJ (2026), 13, 132-145] focuses on the stereochemical analysis of the catalytic mechanism of asparaginases with the aim of resolving key ambiguities in the mechanism. These enzymes have found tru...