Multi-crystal native-SAD phasing at 5 keV with a helium environment [0.03%]
使用氦环境的5 keV多晶原生SAD衍射异常分散测相技术
Akira Karasawa,Babak Andi,Martin R Fuchs et al.
Akira Karasawa et al.
De novo structure determination from single-wavelength anomalous diffraction using native sulfur or phospho-rus in biomolecules (native-SAD) is an appealing method to mitigate the labor-intensive production of heavy-atom derivatives and sel...
Slow protein dynamics probed by time-resolved oscillation crystallography at room temperature [0.03%]
室温时间解析振荡晶体学探测缓慢的蛋白质动力学过程
Sylvain Aumonier,Sylvain Engilberge,Nicolas Caramello et al.
Sylvain Aumonier et al.
The development of serial crystallography over the last decade at XFELs and synchrotrons has produced a renaissance in room-temperature macromolecular crystallography (RT-MX), and fostered many technical and methodological breakthroughs des...
Analytic modeling of inhomogeneous-resolution maps in cryo-electron microscopy and crystallography [0.03%]
冷冻电子显微镜和晶体学中不均匀分辨率图的解析建模
Alexandre Urzhumtsev,Vladimir Y Lunin
Alexandre Urzhumtsev
Refinement of macromolecular atomic models versus experimental maps in crystallography and cryo-electron microscopy is a critical step in structure solution. For an appropriate comparison, model maps should mimic the imperfections in the ex...
Sriram Subramaniam,Abhay Kotecha,Joseph H Davis
Sriram Subramaniam
The 2022 meeting of the American Crystallographic Association in Portland was an inspiring event, addressing a range of both conventional and emerging themes in structural biology. The increasing emphasis at the conference on methods outsid...
Comparative structural analyses of the NHL domains from the human E3 ligase TRIM-NHL family [0.03%]
人源E3连接酶TRIM-NHL家族的NHL结构域的比较结构分析
Apirat Chaikuad,Rezart Zhubi,Claudia Tredup et al.
Apirat Chaikuad et al.
Tripartite motif (TRIM) proteins constitute one of the largest subfamilies of the RING-type E3 ubiquitin ligases that play a role in diverse processes from homeostasis and immune response to viral restriction. While TRIM proteins typically ...
Accurate lattice parameters from 3D electron diffraction data. I. Optical distortions [0.03%]
来自三维电子衍射数据的精确晶格参数。一、光学失真
Petr Brázda,Mariana Klementová,Yaşar Krysiak et al.
Petr Brázda et al.
Determination of lattice parameters from 3D electron diffraction (3D ED) data measured in a transmission electron microscope is hampered by a number of effects that seriously limit the achievable accuracy. The distortion of the diffraction ...
Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature [0.03%]
定义室温下金属酶结构的中子、X射线自由电子激光器和同步辐射晶体学的互补性
Tadeo Moreno-Chicano,Leiah M Carey,Danny Axford et al.
Tadeo Moreno-Chicano et al.
Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies. In the case of metalloenzymes ...
The temperature-dependent conformational ensemble of SARS-CoV-2 main protease (Mpro) [0.03%]
SARS-CoV-2主要蛋白酶(Mpro)的温度依赖性构象组合体
Ali Ebrahim,Blake T Riley,Desigan Kumaran et al.
Ali Ebrahim et al.
The COVID-19 pandemic, instigated by the SARS-CoV-2 coronavirus, continues to plague the globe. The SARS-CoV-2 main protease, or Mpro, is a promising target for the development of novel antiviral therapeutics. Previous X-ray crystal structu...
Metal-free enantiomorphic perovskite [dabcoH2]2+[H3O]+Br- 3 and its one-dimensional polar polymorph [0.03%]
无金属手性钙钛矿[dabcoH2]2+[H3O]+Br- 3及其一维极性多晶型体
Armand Budzianowski,Vaclav Petřiček,Andrzej Katrusiak
Armand Budzianowski
The structure and stoichiometry of a new metal-free and ammonium-free compound [dabcoH2]2+H3O+Br- 3 (where [dabcoH2]2+ = 1,4-di-aza-bicyclo-[2.2.2]octane dication) correspond to the general formula ABX 3 characteristic of perovskites. In en...
Organism-specific differences in the binding of ketoprofen to serum albumin [0.03%]
酮洛芬与血清白蛋白结合的物种差异性研究
Mateusz P Czub,Alan J Stewart,Ivan G Shabalin et al.
Mateusz P Czub et al.
Serum albumin is a circulatory transport protein that has a highly conserved sequence and structure across mammalian organisms. Its ligand-binding properties are of importance as albumin regulates the pharmacokinetics of many drugs. Due to ...