CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes [0.03%]
cryo-EM结构揭示了人类MAT酶复合物催化活性的别构调节
Faisal T Khaja,Reedhi Vara,Louie P Aspinall et al.
Faisal T Khaja et al.
S-Adenosyl methionine (SAMe), the biological methyl donor essential for sustaining the life of most complex organisms, is the second most widely used cofactor, after ATP, in biochemical reactions and is synthesized by the enzyme methionine ...
Cryo-EM-guided subtractive optimization of a novel VCP/p97 inhibitor [0.03%]
基于低温电子显微镜结构优化新型VCP/p97抑制剂
Jason Crawford,Ravi Munuganti,Charles Leung et al.
Jason Crawford et al.
We report the cryo-EM structure-guided discovery of GND-135, a novel small-molecule inhibitor of the VCP/p97 AAA ATPase that demonstrates efficient inhibition of VCP/p97 in biochemical, cellular, and pharmacokinetic assays and in a tumor ef...
Edward H Egelman
Edward H Egelman
In this issue of IUCrJ, Subramaniam, Kühlbrandt & Henderson present an overview of the remarkable progress that has been made in electron cryo-microscopy and electron cryo-tomography. ...
Functional role of a tethered domain as a naturally fused cognate partner is demonstrated in a three-domain copper nitrite reductase [0.03%]
三结构城铜硝酸盐还原酶中天然融合的配对伴侣的功能作用被证实是一个拴系区域
Nopphon Petchyam,Allegra Mbouku,Robert R Eady et al.
Nopphon Petchyam et al.
Copper-containing nitrite reductases (CuNiRs) catalyse the reduction of nitrite to nitric oxide and are a key enzyme in the anaerobic ammonium oxidation and denitrification steps of the nitrogen cycle. The recent recognition of the widespre...
A polar Fourier geometric approach to volume-free single-particle 3D reconstruction [0.03%]
基于极坐标傅立叶几何的无体三维重构方法
Cong T S Van,Cyril F Reboul,Joseph J E Caesar et al.
Cong T S Van et al.
We introduce a compact mathematical formulation for the inverse single-particle 3D reconstruction problem, a high-dimensional inverse problem in which millions of parameters are estimated from extremely noisy experimental measurements. Give...
Radostin Danev,Haruaki Yanagisawa,Keitaro Yamashita et al.
Radostin Danev et al.
Atomic resolution in cryo-electron microscopy was first demonstrated six years ago. This was accomplished using 300 kV electron microscopes equipped with new hardware that provided narrower energy spread, aberration correction and energy fi...
Magnus Nørgaard Kløve,Andreas Dueholm Bertelsen,Mads Ry Vogel Jørgensen et al.
Magnus Nørgaard Kløve et al.
High-temperature polymorphs of hafnia (HfO2) are of significant interest in electronics and fuel-cell applications, and stabilization at ambient conditions can be achieved by aliovalent substitution and nanosize effects. Y3+ stabilization o...
Sriram Subramaniam,Werner Kühlbrandt,Richard Henderson
Sriram Subramaniam
The near-universal adoption of electron cryo-microscopy (cryo-EM) by structural and cell biologists has led to exponential growth of the field, especially over the last two decades, with a doubling in the number of deposited electron-micros...
Drop-on-fixed-target reaction initiation approach for serial and time-resolved crystallography [0.03%]
固定靶滴加反应触发的串行时间分辨晶体学研究方法
Jos J A G Kamps,Philip Hinchliffe,Johan Glerup et al.
Jos J A G Kamps et al.
We describe the design and implementation of a drop-on-fixed-target method for time-resolved serial crystallography at both synchrotron and XFEL facilities. A piezoelectric droplet dispensing pipette is employed for addition of picolitre vo...
Continuous invariant-based asymmetries of periodic crystals quantify deviations from higher symmetry [0.03%]
连续不变量对称性量化周期晶体的更高对称性的偏差
Surya Majumder,Daniel Widdowson,Yury Elkin et al.
Surya Majumder et al.
Ideal symmetry is known to break down under almost any noise. One measure of asymmetry in a periodic crystal is the relative multiplicity Z' of geometrically non-equivalent units. However, Z' discontinuously changes under almost any displac...