Differences in the metabolite composition and mechanical properties of extracellular vesicles secreted by hepatic cellular models [0.03%]
肝细胞模型分泌的细胞外囊泡代谢物组成和机械性能差异分析
Felix Royo,David Gil-Carton,Esperanza Gonzalez et al.
Felix Royo et al.
Liver constitutes the major metabolic factory in the organism and is involved in the synthesis, secretion and clearance of many blood-circulating molecules. Previously, we have characterised the protein and RNA cargo of extracellular vesicl...
Engineering extracellular vesicles as novel treatment options: exploiting herpesviral immunity in CLL [0.03%]
工程化细胞外囊泡作为新型治疗方法：利用疱疹病毒免疫治疗CLL
Kathrin Gärtner,Manja Luckner,Gerhard Wanner et al.
Kathrin Gärtner et al.
Extracellular vesicles (EVs) are important mediators of cell-cell communication. Intriguingly, EVs can be engineered and thus exploited for the targeted transfer of functional proteins of interest. Thus, engineered EVs may constitute attrac...
Raman spectroscopy as a quick tool to assess purity of extracellular vesicle preparations and predict their functionality [0.03%]
拉曼光谱作为评估细胞外囊泡制备纯度和预测其功能的快速工具
Alice Gualerzi,Sander Alexander Antonius Kooijmans,Stefania Niada et al.
Alice Gualerzi et al.
Extracellular vesicles (EVs) from a variety of stem cell sources are believed to harbour regenerative capacity, which may be exploited for therapeutic purposes. Because of EV interaction with other soluble secreted factors, EV activity may ...
An experimental strategy unveiling exosomal microRNAs 486-5p, 181a-5p and 30d-5p from hypoxic tumour cells as circulating indicators of high-risk rectal cancer [0.03%]
一种实验策略，揭示缺氧肿瘤细胞来源的外泌体微RNA（486-5p、181a-5p和30d-5p）作为高危直肠癌循环标志物
Tonje Bjørnetrø,Kathrine Røe Redalen,Sebastian Meltzer et al.
Tonje Bjørnetrø et al.
Tumour hypoxia contributes to poor treatment outcome in locally advanced rectal cancer (LARC) and circulating extracellular vesicles (EVs) as potential biomarkers of tumour hypoxia and adverse prognosis have not been fully explored. We exam...
An improved 96 well plate format lipid quantification assay for standardisation of experiments with extracellular vesicles [0.03%]
一种改进的96孔板脂质定量测定法，用于外泌体实验的标准制定
Tamás Visnovitz,Xabier Osteikoetxea,Barbara W Sódar et al.
Tamás Visnovitz et al.
The field of extracellular vesicles (EVs) is an exponentially growing segment of biomedical sciences. However, the problems of normalisation and quantification of EV samples have not been completely solved. Currently, EV samples are standar...
In vitro decidualisation of human endometrial stromal cells is enhanced by seminal fluid extracellular vesicles [0.03%]
人子宫内膜基质细胞的体外蜕膜化可通过精液细胞外囊泡增强
Helena Rodriguez-Caro,Rebecca Dragovic,Mengni Shen et al.
Helena Rodriguez-Caro et al.
Extracellular vesicles are highly abundant in seminal fluids and have a known role enhancing sperm function. Clinical pregnancy rates after IVF treatment are improved after female exposure to seminal fluid. Seminal fluid extracellular vesic...
Functional recovery in photo-damaged human dermal fibroblasts by human adipose-derived stem cell extracellular vesicles [0.03%]
人脂肪组织衍生干细胞外泌体介导的紫外线损伤皮肤成纤维细胞功能恢复研究
Ji Suk Choi,Woo Lee Cho,Yeo Jin Choi et al.
Ji Suk Choi et al.
Ultraviolet-B (UVB) irradiation causes imbalance between dermal matrix synthesis and degradation through aberrant upregulation of matrix metalloproteinases (MMPs), which leads to overall skin photoaging. We investigated the effects of extra...
Exosomes derived from clinical-grade oral mucosal epithelial cell sheets promote wound healing [0.03%]
临床级口腔黏膜上皮细胞片来源的外泌体促进伤口愈合
Sebastian Sjöqvist,Taichi Ishikawa,Daisuke Shimura et al.
Sebastian Sjöqvist et al.
The oral mucosa exhibits unique regenerative properties, sometimes referred to as foetal-like wound healing. Researchers from our institute have used sheets of oral mucosa epithelial cells (OMECs) for regenerative medicine applications incl...
TEM ExosomeAnalyzer: a computer-assisted software tool for quantitative evaluation of extracellular vesicles in transmission electron microscopy images [0.03%]
TEM Exosome Analyzer：一种辅助软件工具，可对透射电子显微镜图像中的细胞外囊泡进行定量评估
Anna Kotrbová,Karel Štěpka,Martin Maška et al.
Anna Kotrbová et al.
Extracellular vesicles (EVs) function as important conveyers of information between cells and thus can be exploited as drug delivery systems or disease biomarkers. Transmission electron microscopy (TEM) remains the gold standard method for ...
Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential [0.03%]
基于超速离心或尺寸排阻色谱法从血浆中分离的小细胞外囊泡的比较：产量、纯度和功能潜力
Kaloyan Takov,Derek M Yellon,Sean M Davidson
Kaloyan Takov
Interest in small extracellular vesicles (sEVs) as functional carriers of proteins and nucleic acids is growing continuously. There are large numbers of sEVs in the blood, but lack of standardised methods for sEV isolation greatly limits ou...