Cédric Battaglino,Iryna Bodnaruk,Paula Czyszczoń et al.
Cédric Battaglino et al.
Lipopolysaccharide (LPS) is the major component of the outer membrane vesicles (OMVs) of Gram-negative bacteria. We hypothesized that OMVs-induced complement activation contributes to the development of systemic inflammatory response syndro...
Magnetic-Assisted Aptamer Selection and Isolation of Migrasomes for Application in Lung Adenocarcinoma Proteomic Analysis [0.03%]
Yixin Xu,Lin Jiao,Qiangying Yi et al.
Yixin Xu et al.
Migrasomes, newly discovered vesicular organelles, hold promise as diagnostic biomarkers and therapeutic targets in various diseases. However, the exploration of their clinical value remains hindered by the complexity of enriching and analy...
Transcatheter Intra-Arterial Delivery of a Platelet-Derived Extracellular Vesicle-Enriched Preparation for Attenuating Skeletal Muscle Ischaemia-Reperfusion Injury in a Rodent Forelimb Model [0.03%]
Omar A Selim,Aida Sarcon,Atta Behfar et al.
Omar A Selim et al.
Extracellular vesicles (EVs) are non-replicating, lipid membrane-bound nanoparticles released by most eukaryotic cells into the extracellular space. When derived from platelets, a subset of EVs can exhibit remarkable anti-inflammatory and a...
In-Solution Characterization of Extracellular Vesicles: A New Approach to Evaluating Antibody Binding and Surface Interactions [0.03%]
Maria-Anthi Kakavoulia,Peter Spies,Tanja J Kutzner et al.
Maria-Anthi Kakavoulia et al.
Extracellular vesicles (EVs) are emerging as diagnostic and therapeutic agents, yet their nanoscale size limits quantitative single-vesicle analysis of surface epitopes and molecular interactions. Flow-Induced Dispersion Analysis (FIDA) is ...
VAMP4/STX8 Mediate the Autophagic Secretion of Mitochondria and Promote TAMs Polarization in HNSCC [0.03%]
Jingcun Shi,Chun Liu,Xiaoning Wang et al.
Jingcun Shi et al.
Under hypoxic conditions, tumour cells engage in autophagic secretion of mitochondria to sustain energy balance and modulate the tumour microenvironment. However, the exact molecular mechanisms remain unclear. This study used a combination ...
Automated Cryo-EM and Supervised Machine Learning Enable Reproducible Characterization of Extracellular Vesicles and Co-Isolating Particles [0.03%]
Agustin Enciso-Martinez,Frank G A Faas,Anja W M de Jong et al.
Agustin Enciso-Martinez et al.
Cryo-electron microscopy (cryo-EM) is the only technique capable of visualizing the lipid bilayer of extracellular vesicles (EVs), enabling their distinction from non-EV particles. However, the application of cryo-EM for EV sample character...
Leveraging Quantitative Proteomics and Extracellular Vesicle Data to Uncover Druggable Receptor Kinases Across Cancers [0.03%]
Jina Kim,Su Yeon Yeon,Kyerim Choi et al.
Jina Kim et al.
Extracellular vesicles (EVs) play an important role in cancer progression and metastasis. The increasing clinical proteomics data provides an opportunity to uncover new biomarkers and therapeutic targets in cancer specific EVs. Here, we pre...
Proteomic Profiling of Human Extracellular Vesicles Reveals Diagnostic Biomarkers for Colon Adenocarcinoma [0.03%]
Yura Seo,Yoon Dae Han,Linda Bojmar et al.
Yura Seo et al.
Early detection of colon adenocarcinoma (COAD) remains suboptimal. Fecal tests fail to diagnose 30% of stage I cancer, and serum CEA lacks sensitivity (< 40%). Extracellular vesicles (EVs) circulate systemically and package tumor-related ca...
PREDICTOR: A Non-Enzymatic Catalytic Cascade Tool for in Situ Visualization of Small Extracellular Vesicle Surface glycoRNAs [0.03%]
Shuang Xie,Ben Niu,Ruijia Deng et al.
Shuang Xie et al.
Glycosylated RNAs (glycoRNAs) are membrane-displayed RNA-glycan conjugates, but quantitative in situ analysis of low-abundance glycoRNAs on small extracellular vesicles (sEVs) remains challenging. Here, we develop PREDICTOR (proximity-encod...
Nano-Flow Cytometry of Single Extracellular Vesicles Reveals Subpopulation Differences Across Cell Types and Pharmacological Perturbations [0.03%]
Nathalie Nevo,Alix Zhou,Nicolas Ansart et al.
Nathalie Nevo et al.
Extracellular vesicles (EVs) are lipid bilayer-enclosed particles released by most cell types, which can transfer signals and cargoes between cells. EVs released by a single donor cell source are increasingly recognised as extremely heterog...