Investigating the Cytoskeleton of DRGs Using Cryo-Electron Microscopy and Deep Learning [0.03%]
利用 cryo-EM 和深度学习研究 DRG 的细胞骨架
M Neal Waxham,Mihir Relan,Matthew T Swulius et al.
M Neal Waxham et al.
We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo-EM). Cryo-EM reveals the dimensions, proximity, and ove...
Shivani Tuli,Preet Patel,Aneri Shethji et al.
Shivani Tuli et al.
Mitochondrial dysfunction and cytoskeletal disorganization are widely recognized hallmarks of neurodegenerative diseases such as Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), and amyotrophic lateral sclerosi...
Jana Campbell,Cyril Barinka
Jana Campbell
Tubulin C-terminal tails undergo diverse post-translational modifications that regulate microtubule interactions with motors and severing enzymes. TTLL11, a member of the tubulin tyrosine ligase-like (TTLL) family, uniquely catalyzes glutam...
Expression, Purification, and Microscopy-Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin [0.03%]
重组工程蛋氨酸化、脱蛋氨酸化和Δ2人β管蛋白的表达、纯化及显微镜检测方法
Jiayi Chen,Agnieska Szyk,Antonina Roll-Mecak
Jiayi Chen
Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational mo...
LamelliQuant: An Automated Analysis Pipeline for Lamellipodia Protrusion Dynamics [0.03%]
LamelliQuant：用于分析细胞伪足动态突起的自动化数据分析流程
Ian Eder,Abrahim Kashkoush,Partha Roy
Ian Eder
We developed LamelliQuant, an automated image analysis pipeline for quantifying lamellipodial protrusion and retraction dynamics from time-lapse microscopy of migrating cells. The workflow begins with the generation of kymographs along user...
Michaela Horger
Michaela Horger