Visualizing Specific Tubulin Isotypes and Pathogenic Variants in Cellular Microtubule Arrays [0.03%]
可视化细胞微管阵列中的特定角蛋白同种型和致病变异型
Sareen Fiaz,Benjamin Powers,Jayne Aiken
Sareen Fiaz
Eukaryotic cells depend on dynamic microtubule arrays to execute a wide range of functions vital for life. These microtubule filaments are formed through the polymerization of α/β-tubulin proteins, which can be generated from numerous tub...
Double Sided Traction Force Microscopy: A Method to Confine Cells for Physiologically Relevant Force Measurements [0.03%]
双面牵引力显微镜技术：一种用于生理相关力学测量的细胞限域方法
Alexia Caillier,Patrick W Oakes
Alexia Caillier
In this paper we describe a technique to make a confined environment of variable stiffness that is suitable for high-resolution live-cell imaging. This versatile and adaptable technique enables cell confinement between soft elastic surfaces...
Thomas D Pollard
Thomas D Pollard
This perspective summarizes what has been learned about how to do actin filament polymerization experiments that produce data suitable for quantitative analysis. ...
Tracking Mitotic Spindle Dynamics and Protein Localization in Fission Yeast With FYSKA, the Fission Yeast Spindle Kymograph Analyzer [0.03%]
利用分生酵母细丝骨架分析器(FYSKA)追踪分生孢子酵母的分裂丝状体动态及蛋白质定位分布状况
Bojun Zhou,Zachary R Gergely,Michele H Jones et al.
Bojun Zhou et al.
Quantitative analysis of mitotic spindle dynamics requires accurate tracking despite challenges such as cell drift, spindle rotation, and fluctuating fluorescence signals. We developed the Fission Yeast Spindle Kymograph Analyzer (FYSKA), a...
Arp2/3 Inhibition Suppresses Membrane Dynamics but Does Not Block Pigment Granule Migration in Isolated Fish Retinal Pigment Epithelial (RPE) Cells [0.03%]
Arp2/3抑制抑制了膜动力学，但不影响分离的鱼类视网膜色素上皮(RPE)细胞中的色素颗粒迁移
Keelan O&#x;Reilly,Taiki Tashiro,Kristina Kristo et al.
Keelan O&#x;Reilly et al.
The retinal pigment epithelium (RPE) lies at the back of the vertebrate eye and plays important roles in vision. In the eyes of fish, which lack dilatable pupils, RPE pigment granules undergo massive migrations to regulate light intensity. ...
Engineering and Characterization of a Fluorescent Fission Yeast Arp2/3 Complex for Single Molecule Mechanistic Investigations [0.03%]
用于单分子机制研究的荧光裂殖酵母Arp2/3复合物的工程和表征
Caitlin A Anderson,Meghan E O&#x;Connell,Kaitlin E Homa et al.
Caitlin A Anderson et al.
Arp2/3 complex is a seven-component protein complex that facilitates the assembly of branched actin filament networks by binding to pre-existing "mother" actin filaments and initiating the nucleation of new branched "daughter" filaments. Ar...
Jivesh Garg
Jivesh Garg
Tomáš Bárta,Michaela Bosáková,Marcela Buchtová et al.
Tomáš Bárta et al.
Catarina Peneda
Catarina Peneda