Coilin and Cajal bodies [0.03%]
科伊利ん小体和卡亚尔小体
David Staněk
David Staněk
The nucleus of higher eukaryotes contains a number of structures that concentrate specific biomolecules and play distinct roles in nuclear metabolism. In recent years, the molecular mechanisms controlling their formation have been intensive...
Interdependent changes of nuclear lamins, nuclear pore complexes, and ploidy regulate cellular regeneration and stress response in the heart [0.03%]
核纤层、核孔复合体和倍性相互依赖的变化调节心脏细胞再生和应激反应
Yao Li,Alberto Bertozzi,Mellissa Rw Mann et al.
Yao Li et al.
In adult mammals, many heart muscle cells (cardiomyocytes) are polyploid, do not proliferate (post-mitotic), and, consequently, cannot contribute to heart regeneration. In contrast, fetal and neonatal heart muscle cells are diploid, prolife...
A survey of the specificity and mechanism of 1,6 hexanediol-induced disruption of nuclear transport [0.03%]
1,6-己二醇诱导核运输干扰的特异性和机制综述研究
Elizabeth C Riquelme Barrientos,Tegan A Otto,Sara N Mouton et al.
Elizabeth C Riquelme Barrientos et al.
Selective transport through the nuclear pore complex (NPC) depends on the dynamic binding of FG-repeat containing nucleoporins, the FG-nups, with each other and with Karyopherins (Kaps). Here, we assessed the specificity and mechanism by wh...
Where and when to start: Regulating DNA replication origin activity in eukaryotic genomes [0.03%]
何处何时开始起始：真核基因组DNA复制起始位点活性的调控
Clare S K Lee,Matthias Weiβ,Stephan Hamperl
Clare S K Lee
In eukaryotic genomes, hundreds to thousands of potential start sites of DNA replication named origins are dispersed across each of the linear chromosomes. During S-phase, only a subset of origins is selected in a stochastic manner to assem...
Transcriptional condensates and phase separation: condensing information across scales and mechanisms [0.03%]
转录凝聚体和相分离：跨尺度和机制地凝缩信息
Justin Demmerle,Siyuan Hao,Danfeng Cai
Justin Demmerle
Transcription is the fundamental process of gene expression, which in eukaryotes occurs within the complex physicochemical environment of the nucleus. Decades of research have provided extreme detail in the molecular and functional mechanis...
The interplay between viral molecular mimicry and host chromatin dynamics [0.03%]
病毒分子模拟与宿主染色质动力学的相互作用
Shumin Xiao,Yajing Wang,Shan Shan et al.
Shumin Xiao et al.
Molecular mimicry is a commonly used mechanism by viruses to manipulate host cellular machinery and coordinate their life cycles. While histone mimicry is well studied, viruses also employ other mimicry strategies to affect chromatin dynami...
Rajat Mann,Dimple Notani
Rajat Mann
Transcription Factor (TF) condensates are a heterogenous mix of RNA, DNA, and multiple co-factor proteins capable of modulating the transcriptional response of the cell. The dynamic nature and the spatial location of TF-condensates in the 3...
Lamin B1 overexpression alters chromatin organization and gene expression [0.03%]
lamin B1过度表达改变染色质结构和基因表达
Jeanae M Kaneshiro,Juliana S Capitanio,Martin W Hetzer
Jeanae M Kaneshiro
Peripheral heterochromatin positioning depends on nuclear envelope associated proteins and repressive histone modifications. Here we show that overexpression (OE) of Lamin B1 (LmnB1) leads to the redistribution of peripheral heterochromatin...
Differential contributions of nuclear lamina association and genome compartmentalization to gene regulation [0.03%]
核小体重塑对基因调控的贡献多于染色质区室化的影响
Priyojit Das,Rebeca San Martin,Rachel Patton McCord
Priyojit Das
Chromatin regions that interact with the nuclear lamina are often heterochromatic, repressed in gene expression, and in the spatial B compartment. However, exceptions to this trend allow us to examine the relative impact of lamin associatio...
Improved protocol for single-nucleus RNA-sequencing of frozen human bladder tumor biopsies [0.03%]
改良的冷冻人膀胱肿瘤活检单核RNA测序技术协议
Sofie S Schmøkel,Iver Nordentoft,Sia V Lindskrog et al.
Sofie S Schmøkel et al.
This paper provides a laboratory workflow for single-nucleus RNA-sequencing (snRNA-seq) including a protocol for gentle nuclei isolation from fresh frozen tumor biopsies, making it possible to analyze biobanked material. To develop this pro...