Gaussian curvature dilutes the nuclear lamina, favoring nuclear rupture, especially at high strain rate [0.03%]
高应变率下高斯曲率通过削弱核纤层从而促进细胞核破裂的作用机制研究
Charlotte R Pfeifer,Michael P Tobin,Sangkyun Cho et al.
Charlotte R Pfeifer et al.
Nuclear rupture has long been associated with deficits or defects in lamins, with recent results also indicating a role for actomyosin stress, but key physical determinants of rupture remain unclear. Here, lamin-B filaments stably interact ...
In situ nuclear matrix preparation in Drosophila melanogaster embryos/tissues and its use in studying the components of nuclear architecture [0.03%]
果蝇胚胎/组织体内核骨架的制备及其在研究核结构组分中的应用
Rashmi U Pathak,Ashish Bihani,Rahul Sureka et al.
Rashmi U Pathak et al.
The study of nuclear matrix (NuMat) over the last 40 years has been limited to either isolated nuclei from tissues or cells grown in culture. Here, we provide a protocol for NuMat preparation in intact Drosophila melanogaster embryos and it...
Mammalian nuclear speckles exhibit stable association with chromatin: a biochemical study [0.03%]
哺乳动物的细胞核斑点与染色质具有稳定关联：生化研究
Komal Raina,Basuthkar J Rao
Komal Raina
Nuclear Speckles (NS) are phase-separated condensates of protein and RNA whose components dynamically coordinate RNA transcription, splicing, transport and DNA repair. NS, probed largely by imaging studies, remained historically well known ...
Rajiv Kumar Jha,David Levens,Fedor Kouzine
Rajiv Kumar Jha
The compaction of linear DNA into micrometer-sized nuclear boundaries involves the establishment of specific three-dimensional (3D) DNA structures complexed with histone proteins that form chromatin. The resulting structures modulate essent...
Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae [0.03%]
Uip4p调节模式生物酿酒酵母核孔复合体的功能
Pallavi Deolal,Imlitoshi Jamir,Krishnaveni Mishra
Pallavi Deolal
A double membrane bilayer perforated by nuclear pore complexes (NPCs) governs the shape of the nucleus, the prominent distinguishing organelle of a eukaryotic cell. Despite the absence of lamins in yeasts, the nuclear morphology is stably m...
Pericentromeric repetitive ncRNA regulates chromatin interaction and inflammatory gene expression [0.03%]
近着丝粒重复性ncRNA调节染色质互作和炎症基因表达
Kenichi Miyata,Akiko Takahashi
Kenichi Miyata
Cellular senescence provokes a dramatic alteration of chromatin organization and gene expression profile of proinflammatory factors, thereby contributing to various age-related pathologies via the senescence-associated secretory phenotype (...
MeCP2-induced heterochromatin organization is driven by oligomerization-based liquid-liquid phase separation and restricted by DNA methylation [0.03%]
DNA甲基化限制下的MeCP2诱导的异染色质组织由寡聚诱导的液-液相分离驱动
Hui Zhang,Hector Romero,Annika Schmidt et al.
Hui Zhang et al.
Heterochromatin is the highly compacted form of chromatin with various condensation levels hallmarked by high DNA methylation. MeCP2 is mostly known as a DNA methylation reader but has also been reported as a heterochromatin organizer. Here...
Architectural control of mesenchymal stem cell phenotype through nuclear actin [0.03%]
通过核内肌动蛋白控制间充质干细胞的表型
Janet Rubin,Andre J van Wijnen,Gunes Uzer
Janet Rubin
There is growing appreciation that architectural components of the nucleus regulate gene accessibility by altering chromatin organization. While nuclear membrane connector proteins link the mechanosensitive actin cytoskeleton to the nucleos...
Filament assembly of the C. elegans lamin in the absence of helix 1A [0.03%]
无第1A螺旋时C.elegans核纤层蛋白的丝状组装
Rebecca de Leeuw,Rafael Kronenberg-Tenga,Matthias Eibauer et al.
Rebecca de Leeuw et al.
Lamins are the major constituent of the nuclear lamina, a protein meshwork underlying the inner nuclear membrane. Nuclear lamins are type V intermediate filaments that assemble into ~3.5 nm thick filaments. To date, only the conditions for ...
Nuclear envelope mechanobiology: linking the nuclear structure and function [0.03%]
核膜力学生物学：连接核结构与功能
Matthew Goelzer,Julianna Goelzer,Matthew L Ferguson et al.
Matthew Goelzer et al.
The nucleus, central to cellular activity, relies on both direct mechanical input as well as its molecular transducers to sense external stimuli and respond by regulating intra-nuclear chromatin organization that determines cell function an...