Robust and heritable knockdown of gene expression using a self-cleaving ribozyme in Drosophila [0.03%]
在果蝇中使用自我切割核酶实现稳健和可遗传的基因表达抑制
Kevin G Nyberg,Fritz Gerald Navales,Eren Keles et al.
Kevin G Nyberg et al.
The current toolkit for genetic manipulation in the model animal Drosophila melanogaster is extensive and versatile but not without its limitations. Here, we report a powerful and heritable method to knockdown gene expression in D. melanoga...
Spontaneous and double-strand break repair-associated quasipalindrome and frameshift mutagenesis in budding yeast: role of mismatch repair [0.03%]
酵母菌中自发的和双链断裂修复相关的拟回文突变与框移突变的作用及错配修复的作用
Neal Sugawara,Mason J Towne,Susan T Lovett et al.
Neal Sugawara et al.
Although gene conversion (GC) in Saccharomyces cerevisiae is the most error-free way to repair double-strand breaks (DSBs), the mutation rate during homologous recombination is 1,000 times greater than during replication. Many mutations inv...
The Dmc1 recombinase physically interacts with and promotes the meiotic crossover functions of the Mlh1-Mlh3 endonuclease [0.03%]
Dmc1重组酶可与Mei-4/Mlh1-Mlh3内切酶相互作用并促进其介导的交配型转换和减数分裂交叉互换功能
Gianno Pannafino,Jun Jie Chen,Viraj Mithani et al.
Gianno Pannafino et al.
The accurate segregation of homologous chromosomes during the Meiosis I reductional division in most sexually reproducing eukaryotes requires crossing over between homologs. In baker's yeast approximately 80% of meiotic crossovers result fr...
MAB-5/Hox regulates the Q neuroblast transcriptome, including cwn-1/Wnt, to mediate posterior migration in Caenorhabditis elegans [0.03%]
C. elegans 中 MAB-5/Hox 通过调节 Q 神经前体细胞转录组（包括 cwn-1/Wnt）介导迁移至后部
Vitoria K Paolillo,Matthew E Ochs,Erik A Lundquist
Vitoria K Paolillo
Neurogenesis involves the precisely coordinated action of genetic programs controlling large-scale neuronal fate specification down to terminal events of neuronal differentiation. The Q neuroblasts in Caenorhabditis elegans, QL on the left ...
Zhengkuan Sun,Sachi Inagaki,Keita Miyoshi et al.
Zhengkuan Sun et al.
Nanostructures of pores and protrusions in the insect cuticle modify molecular permeability and surface wetting and help insects sense various environmental cues. However, the cellular mechanisms that modify cuticle nanostructures are poorl...
Ploidy inference from single-cell data: application to human and mouse cell atlases [0.03%]
从单细胞数据中推断染色体倍性：在人类和小鼠细胞图谱中的应用
Fumihiko Takeuchi,Norihiro Kato
Fumihiko Takeuchi
Ploidy is relevant to numerous biological phenomena, including development, metabolism, and tissue regeneration. Single-cell RNA-seq and other omics studies are revolutionizing our understanding of biology, yet they have largely overlooked ...
Evolutionary graph theory beyond single mutation dynamics: on how network-structured populations cross fitness landscapes [0.03%]
超越单次变异进化图论：网络化种群如何跨越适应度景观
Yang Ping Kuo,Oana Carja
Yang Ping Kuo
Spatially resolved datasets are revolutionizing knowledge in molecular biology, yet are under-utilized for questions in evolutionary biology. To gain insight from these large-scale datasets of spatial organization, we need mathematical repr...
Identification of factors limiting the allotopic production of the Cox2 subunit of yeast cytochrome c oxidase [0.03%]
鉴定影响酵母细胞色素c氧化酶Cox2亚基异位表达的因素
Felipe Nieto-Panqueva,Miriam Vázquez-Acevedo,Patrice P Hamel et al.
Felipe Nieto-Panqueva et al.
Mitochondrial genes can be artificially relocalized in the nuclear genome in a process known as allotopic expression, such is the case of the mitochondrial cox2 gene, encoding subunit II of cytochrome c oxidase (CcO). In yeast, cox2 can be ...
Harnessing the power of new genetic tools to illuminate Giardia biology and pathogenesis [0.03%]
利用新型基因工具揭示隐孢子虫生物学和致病机制
Kari D Hagen,Christopher J S Hart,Shane G McInally et al.
Kari D Hagen et al.
Giardia is a prevalent single-celled microaerophilic intestinal parasite causing diarrheal disease and significantly impacting global health. Double diploid (essentially tetraploid) Giardia trophozoites have presented a formidable challenge...